Q06AT3 (UCHL5_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Short name=UCH-L5
EC=3.4.19.12

Alternative name(s):
Ubiquitin thioesterase L5

Gene names

Name:

UCHL5

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Enzyme regulation	Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.
Subunit structure	Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	omega peptidase activity Inferred from electronic annotation. Source: InterPro ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Ubiquitin carboxyl-terminal hydrolase isozyme L5		PRO_0000288777
Regions
	Region	313 – 329	17	Interaction with ADRM1 By similarity
Sites
	Active site	88	1	Nucleophile By similarity
	Active site	164	1	Proton donor By similarity
	Site	179	1	Important for enzyme activity By similarity
Amino acid modifications
	Modified residue	158	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06AT3 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: EAB066D16BA51EA5	FASTA	329	37,577
	10 20 30 40 50 60 MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 70 80 90 100 110 120 GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 130 140 150 160 170 180 FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTSAKEE DAFHFVSYVP VNGRLYELDG 190 200 210 220 230 240 LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 250 260 270 280 290 300 QLAEEEPMDT DQGSNMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 310 320 KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

References

[1]	Liu G.Y. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ917642 mRNA. Translation: ABI97187.1.
RefSeq	NP_001116682.1. NM_001123210.1.
UniGene	Ssc.8201.
3D structure databases
ProteinModelPortal	Q06AT3.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000011515.
Protein family/group databases
MEROPS	C12.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100144472.
KEGG	ssc:100144472.
Organism-specific databases
CTD	51377.
Phylogenomic databases
eggNOG	NOG321645.
HOGENOM	HOG000203918.
HOVERGEN	HBG056021.
KO	K05610.
OrthoDB	EOG4RBQK0.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. IPR017390. Ubiquitinyl_hydrolase_UCH37. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PIRSF	PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTS	PR00707. UBCTHYDRLASE.
ProtoNet	Search...

Entry information

Entry name

UCHL5_PIG

Accession

Primary (citable) accession number: Q06AT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 31, 2006
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program