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Q06AT3 (UCHL5_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5

Short name=UCH-L5
EC=3.4.19.12
Alternative name(s):
Ubiquitin thioesterase L5
Gene names
Name:UCHL5
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.

Subunit structure

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Ubiquitin carboxyl-terminal hydrolase isozyme L5
PRO_0000288777

Regions

Region313 – 32917Interaction with ADRM1 By similarity

Sites

Active site881Nucleophile By similarity
Active site1641Proton donor By similarity
Site1791Important for enzyme activity By similarity

Amino acid modifications

Modified residue471N6-succinyllysine By similarity
Modified residue1581N6-acetyllysine By similarity
Modified residue2891N6-succinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06AT3 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: EAB066D16BA51EA5

FASTA32937,577
        10         20         30         40         50         60 
MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 

        70         80         90        100        110        120 
GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 

       130        140        150        160        170        180 
FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTSAKEE DAFHFVSYVP VNGRLYELDG 

       190        200        210        220        230        240 
LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 

       250        260        270        280        290        300 
QLAEEEPMDT DQGSNMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 

       310        320 
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK 

« Hide

References

[1]Liu G.Y.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ917642 mRNA. Translation: ABI97187.1.
RefSeqNP_001116682.1. NM_001123210.1.
UniGeneSsc.8201.

3D structure databases

ProteinModelPortalQ06AT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000011515.

Protein family/group databases

MEROPSC12.005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100144472.
KEGGssc:100144472.

Organism-specific databases

CTD51377.

Phylogenomic databases

eggNOGNOG321645.
HOGENOMHOG000203918.
HOVERGENHBG056021.
KOK05610.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSPR00707. UBCTHYDRLASE.
ProtoNetSearch...

Entry information

Entry nameUCHL5_PIG
AccessionPrimary (citable) accession number: Q06AT3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 31, 2006
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries