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Q06AK7

- TOP1_STAAU

UniProt

Q06AK7 - TOP1_STAAU

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg(2+) per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi82 – 821Magnesium 1; catalyticUniRule annotation
Metal bindingi82 – 821Magnesium 2UniRule annotation
Metal bindingi84 – 841Magnesium 2UniRule annotation
Sitei139 – 1391Interaction with DNAUniRule annotation
Sitei140 – 1401Interaction with DNAUniRule annotation
Sitei143 – 1431Interaction with DNAUniRule annotation
Sitei148 – 1481Interaction with DNAUniRule annotation
Sitei155 – 1551Interaction with DNAUniRule annotation
Active sitei298 – 2981O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei300 – 3001Interaction with DNAUniRule annotation
Sitei488 – 4881Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri577 – 60327C4-type 1Add
BLAST
Zinc fingeri617 – 64529C4-type 2Add
BLAST
Zinc fingeri658 – 68124C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689DNA topoisomerase 1PRO_0000285934Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ06AK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 113111ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1686Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri577 – 60327C4-type 1Add
BLAST
Zinc fingeri617 – 64529C4-type 2Add
BLAST
Zinc fingeri658 – 68124C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06AK7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDTEDNY
60 70 80 90 100
EPKYITIRGK GPVVKELKKH AKKAKNVFLA SDPDREGEAI AWHLSKILEL
110 120 130 140 150
EDSKENRVVF NEITKDAVKE SFKNPREIEM NLVDAQQARR ILDRLVGYNI
160 170 180 190 200
SPVLWKKVKK GLSAGRVQSV ALRLVIDREN EIRNFKPEEY WTIEGEFRYK
210 220 230 240 250
KSKFNAKFLH YKNKPFKLKT KKDVEKITTA LDGDQFEITN VTKKEKTRNP
260 270 280 290 300
ANPFTTSTLQ QEAARKLNFK ARKTMMVAQQ LYEGIDLKKQ GTIGLITYMR
310 320 330 340 350
TDSTRISDTA KAEAKQYITN KYGESYTSKR KASGKQGDQD AHEAIRPSST
360 370 380 390 400
MRTPDDMKSF LTKDQYRLYK LIWERFVASQ MAPAILDTVS LDITQGDIKF
410 420 430 440 450
RANGQTIKFK GFMTLYVETK DDSDSEKENK LPKLEQGDKV TATQIEPAQH
460 470 480 490 500
YTQPPPRYTE ARLVKTLEEL KIGRPSTYAP TIDTIQKRNY VKLESKRFVP
510 520 530 540 550
TELGEIVHEQ VKEYFPEIID VEFTVNMETL LDKIAEGDIT WRKVIDGFFS
560 570 580 590 600
SFKQDVERAE EEMEKIEIKD EPAGEDCEVC GSPMVIKMGR YGKFMACSNF
610 620 630 640 650
PDCRNTKAIV KSIGVKCPKC NDGDVVERKS KKNRVFYGCS KYPECDFISW
660 670 680
DKPIGRDCPK CNQYLVENKK GKTTQVICSN CDYKEAAQK
Length:689
Mass (Da):79,100
Last modified:October 31, 2006 - v1
Checksum:i3A42A3B958794887
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ923160 Genomic DNA. Translation: ABI81408.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ923160 Genomic DNA. Translation: ABI81408.1 .

3D structure databases

ProteinModelPortali Q06AK7.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB01051. Novobiocin.
DB00487. Pefloxacin.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Staphylococcus aureus topoisomerase I."
    Park J.E., Kim H.I., Lee J.S.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C66.

Entry informationi

Entry nameiTOP1_STAAU
AccessioniPrimary (citable) accession number: Q06AK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 31, 2006
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3