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Q06AB3

- UCHL3_PIG

UniProt

Q06AB3 - UCHL3_PIG

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L3

Gene

UCHL3

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3, and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Inhibited by monoubiquitin and diubiquitin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951NucleophilePROSITE-ProRule annotation
    Active sitei169 – 1691Proton donorPROSITE-ProRule annotation
    Sitei184 – 1841Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BRENDAi3.4.19.12. 6170.

    Protein family/group databases

    MEROPSiC12.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L3 (EC:3.4.19.12)
    Short name:
    UCH-L3
    Alternative name(s):
    Ubiquitin thioesterase L3
    Gene namesi
    Name:UCHL3
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230Ubiquitin carboxyl-terminal hydrolase isozyme L3PRO_0000289800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei130 – 1301PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ06AB3.
    SMRiQ06AB3. Positions 2-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 136Interaction with ubiquitinBy similarity
    Regioni152 – 1598Interaction with ubiquitinBy similarity
    Regioni219 – 2246Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    HOVERGENiHBG075483.
    KOiK05609.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06AB3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC    50
    AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TPSVYFMKQT ISNACGTIGL 100
    IHAIANNKNK MHFESGSTLK KFLEESASMS PDERARYLES YDAIPVTHET 150
    SAHEGQTEAP NIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET 200
    LLEDAIEVCK KFMERDPDEL RFNAIALSAA 230
    Length:230
    Mass (Da):26,090
    Last modified:October 31, 2006 - v1
    Checksum:i1C12B99BAA02E427
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ972951 mRNA. Translation: ABI96187.1.
    RefSeqiNP_001070695.1. NM_001077227.1.
    UniGeneiSsc.13668.

    Genome annotation databases

    GeneIDi768115.
    KEGGissc:768115.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ972951 mRNA. Translation: ABI96187.1 .
    RefSeqi NP_001070695.1. NM_001077227.1.
    UniGenei Ssc.13668.

    3D structure databases

    ProteinModelPortali Q06AB3.
    SMRi Q06AB3. Positions 2-230.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 768115.
    KEGGi ssc:768115.

    Organism-specific databases

    CTDi 7347.

    Phylogenomic databases

    HOVERGENi HBG075483.
    KOi K05609.

    Enzyme and pathway databases

    BRENDAi 3.4.19.12. 6170.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Liu G.Y.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiUCHL3_PIG
    AccessioniPrimary (citable) accession number: Q06AB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3