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Q06965 (HEM0_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-aminolevulinate synthase 2

EC=2.3.1.37
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene names
Name:hemT
Ordered Locus Names:RHOS4_30750
ORF Names:RSP_3028
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Caution

Expressed in the mutant strain HemA1 but expression in the wild-type strain has not been proven.

Ontologies

Keywords
   Biological processHeme biosynthesis
   LigandPyridoxal phosphate
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function5-aminolevulinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4074075-aminolevulinate synthase 2
PRO_0000163830

Sites

Active site2481 By similarity
Binding site211Substrate By similarity
Binding site1371Substrate By similarity
Binding site1891Pyridoxal phosphate By similarity
Binding site2171Pyridoxal phosphate By similarity
Binding site2451Pyridoxal phosphate By similarity
Binding site2771Pyridoxal phosphate By similarity
Binding site2781Pyridoxal phosphate By similarity
Binding site3631Substrate By similarity

Amino acid modifications

Modified residue2481N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06965 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 4772E3EC1DA55D82

FASTA40744,333
        10         20         30         40         50         60 
MEFSQHFQKL IDDMRLDGRY RTFAELERIA GEFPTALWHG PDGQARRVTV WCSNDYLGMG 

        70         80         90        100        110        120 
QNAEVLAAMH RSIDLSGAGT GGTRNISGTN RQHVALEAEL ADLHGKESAL IFTSGWISNL 

       130        140        150        160        170        180 
AALGTLGKIL PECAIFSDAL NHNSMIEGIR RSGAERFIFH HNDPVHLDRL LSSVDPARPK 

       190        200        210        220        230        240 
IVAFESVYSM DGDIAPIAEI CDVAERHGAL TYLDEVHAVG LYGPRGGGIS DRDGLADRVT 

       250        260        270        280        290        300 
IIEGTLAKAF GVMGGYVSGP SLLMDVIRSM SDSFIFTTSI CPHLAAGALA AVRHVKAHPD 

       310        320        330        340        350        360 
ERRRQAENAV RLKVLLQKAG LPVLDTPSHI LPVMVGEAHL CRSISEALLA RHAIYVQPIN 

       370        380        390        400 
YPTVARGQER FRLTPTPFHT TSHMEALVEA LLAVGRDLGW AMSRRAA 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes."
Neidle E.L., Kaplan S.
J. Bacteriol. 175:2292-2303(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 2 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07489 Genomic DNA. Translation: AAA26124.1.
CP000144 Genomic DNA. Translation: ABA80643.1.
PIRA49845.
RefSeqYP_354544.1. NC_007494.2.

3D structure databases

ProteinModelPortalQ06965.
SMRQ06965. Positions 2-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272943.RSP_3028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA80643; ABA80643; RSP_3028.
GeneID3721613.
KEGGrsp:RSP_3028.
PATRIC23156193. VBIRhoSph57909_3445.

Phylogenomic databases

eggNOGCOG0156.
HOGENOMHOG000221020.
KOK00643.
OMASHMEALV.
OrthoDBEOG6Q8HZD.
ProtClustDBPRK13393.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13234.
RSPH272943:GJAS-3162-MONOMER.
UniPathwayUPA00251; UER00375.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01821. 5aminolev_synth. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM0_RHOS4
AccessionPrimary (citable) accession number: Q06965
Secondary accession number(s): Q3IXU1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 22, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways