Reviewed, UniProtKB/Swiss-Prot Q06965 (HEM0_RHOS4)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase 2
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:	hemT
Ordered Locus Names:	RHOS4_30750
ORF Names:	RSP_3028

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Complete proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Caution	Expressed in the mutant strain HemA1 but expression in the wild-type strain has not been proven.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	5-aminolevulinate synthase 2		PRO_0000163830
Amino acid modifications
	Modified residue	248	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06965-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4772E3EC1DA55D82	FASTA	407	44,333
	10 20 30 40 50 60 MEFSQHFQKL IDDMRLDGRY RTFAELERIA GEFPTALWHG PDGQARRVTV WCSNDYLGMG 70 80 90 100 110 120 QNAEVLAAMH RSIDLSGAGT GGTRNISGTN RQHVALEAEL ADLHGKESAL IFTSGWISNL 130 140 150 160 170 180 AALGTLGKIL PECAIFSDAL NHNSMIEGIR RSGAERFIFH HNDPVHLDRL LSSVDPARPK 190 200 210 220 230 240 IVAFESVYSM DGDIAPIAEI CDVAERHGAL TYLDEVHAVG LYGPRGGGIS DRDGLADRVT 250 260 270 280 290 300 IIEGTLAKAF GVMGGYVSGP SLLMDVIRSM SDSFIFTTSI CPHLAAGALA AVRHVKAHPD 310 320 330 340 350 360 ERRRQAENAV RLKVLLQKAG LPVLDTPSHI LPVMVGEAHL CRSISEALLA RHAIYVQPIN 370 380 390 400 YPTVARGQER FRLTPTPFHT TSHMEALVEA LLAVGRDLGW AMSRRAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes." Neidle E.L., Kaplan S. J. Bacteriol. 175:2292-2303(1993) [PubMed: 8468290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 2 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07489 Genomic DNA. Translation: AAA26124.1. CP000144 Genomic DNA. Translation: ABA80643.1.
PIR	A49845.
RefSeq	YP_354544.1.
3D structure databases
HSSP	HSSP built from PDB template 1FC4 based on UniProtKB P07912.
ModBase	Search...
Genome annotation databases
GeneID	3721613.
GenomeReviews	Gene locus RHOS4_30750 in contig CP000144_GR.
KEGG	rsp:RSP_3028.
NMPDR	fig|272943.3.peg.3643.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q06965.
OMA	Q06965. GSRNIGG.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13234. RSPH272943:RSP_3028-MON.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM0_RHOS4

Accession

Primary (citable) accession number: Q06965
Secondary accession number(s): Q3IXU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)