ID   GM4D1_VIBCH             Reviewed;         373 AA.
AC   Q06952;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Probable GDP-mannose 4,6-dehydratase;
DE            EC=4.2.1.47;
DE   AltName: Full=GDP-D-mannose dehydratase;
GN   Name=rfbD; Synonyms=gmd; OrderedLocusNames=VC_0243;
OS   Vibrio cholerae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor O17 / Serotype O1;
RX   MEDLINE=92212870; PubMed=1372980; DOI=10.1073/pnas.89.7.2566;
RA   Stroeher U.H., Karageorgos L.E., Morona R., Manning P.A.;
RT   "Serotype conversion in Vibrio cholerae O1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:2566-2570(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor O17 / Serotype O1;
RX   MEDLINE=95309704; PubMed=7540582; DOI=10.1016/0378-1119(95)00124-O;
RA   Manning P.A., Stroeher U.H., Karageorgos L.E., Morona R.;
RT   "Putative O-antigen transport genes within the rfb region of Vibrio
RT   cholerae O1 are homologous to those for capsule transport.";
RL   Gene 158:1-7(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961 / Serotype O1;
RX   MEDLINE=20406833; PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A.,
RA   Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L.,
RA   Ermolaeva M.D., Vamathevan J.J., Bass S., Qin H., Dragoi I.,
RA   Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D.,
RA   Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R.,
RA   Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- CATALYTIC ACTIVITY: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose
CC       + H(2)O.
CC   -!- COFACTOR: NAD (By similarity).
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis
CC       via de novo pathway; GDP-L-fucose from GDP-D-mannose: step 1/2.
CC   -!- SIMILARITY: Belongs to the GDP-mannose 4,6-dehydratase family.
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DR   EMBL; X59554; CAA42136.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF93419.1; -; Genomic_DNA.
DR   PIR; S28470; S28470.
DR   RefSeq; NP_229900.1; -.
DR   HSSP; P32054; 1DB3.
DR   GeneID; 2614706; -.
DR   GenomeReviews; AE003852_GR; VC_0243.
DR   KEGG; vch:VC0243; -.
DR   TIGR; VC_0243; -.
DR   HOGENOM; Q06952; -.
DR   OMA; Q06952; LANISQG.
DR   BioCyc; MetaCyc:MON-13574; -.
DR   BRENDA; 4.2.1.47; 19019.
DR   GO; GO:0005622; C:intracellular; IEA:InterPro.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0008446; F:GDP-mannose 4,6-dehydratase activity; IEA:EC.
DR   GO; GO:0019673; P:GDP-mannose metabolic process; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR006368; GDP_Man_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR10366:SF32; GDP_mann_dehyd; 1.
DR   Pfam; PF01370; Epimerase; 1.
DR   TIGRFAMs; TIGR01472; gmd; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lipopolysaccharide biosynthesis; Lyase; NAD.
FT   CHAIN         1    373       Probable GDP-mannose 4,6-dehydratase.
FT                                /FTId=PRO_0000201717.
FT   ACT_SITE    134    134       By similarity.
FT   ACT_SITE    136    136       Nucleophile (By similarity).
FT   ACT_SITE    158    158       Nucleophile (By similarity).
FT   BINDING     162    162       NADP (By similarity).
SQ   SEQUENCE   373 AA;  42053 MW;  4EB10DCAB6A8C1D2 CRC64;
     MNKKVALITG ITGQDGSYLA EFLLEKGYEV HGIKRRSSLF NTQRVDHLYK DPHEEDVNFK
     LHYGDLTDSS NLTRILAEVQ PDEVYNLGAQ SHVAVSFQSP EYTADVDAIG TLRLLEAIRF
     LGLTKKTKFY QASTSELYGL VQEIPQKETT PFYPRSPYAV AKMYAYWITI NYRESYGIYA
     CNGILFNHES PRRGETFVTR KITRGMANIA QGLEKCLFMG NLDALRDWGH AKDYVKMQWM
     MLQQDEPRDF VIATGVQYSV REFIDMSARE LGIELEFVGK GVDEKAVVKS VIGTKAPAIK
     VGDIIVAVDP AYFRPAEVET LLGDPSLAKK ELGWVPEITL QQMVSEMVAS DLEQAQSHAL
     LKKHGYNVNV SVE
//