ID   FL3H_MALDO              Reviewed;         364 AA.
AC   Q06942;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   05-MAY-2009, entry version 47.
DE   RecName: Full=Naringenin,2-oxoglutarate 3-dioxygenase;
DE            EC=1.14.11.9;
DE   AltName: Full=Flavonone-3-hydroxylase;
DE            Short=F3H;
DE   AltName: Full=FHT;
OS   Malus domestica (Apple) (Malus sylvestris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Rosales; Rosaceae; Maloideae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   MEDLINE=94269194; PubMed=8208851; DOI=10.1104/pp.103.1.291;
RA   Davies K.M.;
RT   "A cDNA clone for flavanone 3-hydroxylase from Malus.";
RL   Plant Physiol. 103:291-291(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Fuji; TISSUE=Peelings;
RA   Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R.;
RT   "Molecular cloning and expression of anthocyanin biosynthesis genes
RT   from 'Fuji apple'.";
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to
CC       2R,3R-dihydroflavonols which are intermediates in the biosynthesis
CC       of flavonols, anthocyanidins, catechins and proanthocyanidins in
CC       plants.
CC   -!- CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O(2) = a
CC       dihydroflavonol + succinate + CO(2).
CC   -!- COFACTOR: Iron.
CC   -!- COFACTOR: Ascorbate.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X69664; CAA49353.1; -; mRNA.
DR   EMBL; AF117270; AAD26206.1; -; mRNA.
DR   PIR; S31458; S31458.
DR   BRENDA; 1.14.11.9; 66979.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0045486; F:naringenin 3-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    364       Naringenin,2-oxoglutarate 3-dioxygenase.
FT                                /FTId=PRO_0000067287.
FT   METAL        76     76       Iron (Potential).
FT   METAL       218    218       Iron (Potential).
FT   METAL       220    220       Iron (Potential).
FT   METAL       276    276       Iron (Potential).
SQ   SEQUENCE   364 AA;  40771 MW;  B84793FCF831CE44 CRC64;
     MAPATTLTSI AHEKTLQQKF VRDEDERPKV AYNDFSNEIP IISLAGIDEV EGRRGEICKK
     IVAACEDWGI FQIVDHGVDA ELISEMTGLA REFFALPSEE KLRFDMSGGK KGGFIVSSHL
     QGEAVQDWRE IVTYFSYPIR HRDYSRWPDK PEAWREVTKK YSDELMGLAC KLLGVLSEAM
     GLDTEALTKA CVDMDQKVVV NFYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD
     DGKTWITVQP VEGAFVVNLG DHGHLLSNGR FKNADHQAVV NSNSSRLSIA TFQNPAQEAI
     VYPLSVREGE KPILEAPITY TEMYKKKMSK DLELARLKKL AKEQQSQDLE KAKVDTKPVD
     DIFA
//