Reviewed, UniProtKB/Swiss-Prot Q06942 (FL3H_MALDO)

Names and origin

Protein names	Recommended name:     Naringenin,2-oxoglutarate 3-dioxygenase     EC=1.14.11.9 Alternative name(s):     Flavonone-3-hydroxylase       Short name=F3H     FHT
Organism	Malus domestica (Apple) (Malus sylvestris)
Taxonomic identifier	3750 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O(2) = a dihydroflavonol + succinate + CO(2).
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067287
Sites
	Metal binding	76	1	Iron Potential
	Metal binding	218	1	Iron Potential
	Metal binding	220	1	Iron Potential
	Metal binding	276	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06942-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B84793FCF831CE44	FASTA	364	40,771
	10 20 30 40 50 60 MAPATTLTSI AHEKTLQQKF VRDEDERPKV AYNDFSNEIP IISLAGIDEV EGRRGEICKK 70 80 90 100 110 120 IVAACEDWGI FQIVDHGVDA ELISEMTGLA REFFALPSEE KLRFDMSGGK KGGFIVSSHL 130 140 150 160 170 180 QGEAVQDWRE IVTYFSYPIR HRDYSRWPDK PEAWREVTKK YSDELMGLAC KLLGVLSEAM 190 200 210 220 230 240 GLDTEALTKA CVDMDQKVVV NFYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD 250 260 270 280 290 300 DGKTWITVQP VEGAFVVNLG DHGHLLSNGR FKNADHQAVV NSNSSRLSIA TFQNPAQEAI 310 320 330 340 350 360 VYPLSVREGE KPILEAPITY TEMYKKKMSK DLELARLKKL AKEQQSQDLE KAKVDTKPVD DIFA

References

[1]	"A cDNA clone for flavanone 3-hydroxylase from Malus." Davies K.M. Plant Physiol. 103:291-291(1993) [PubMed: 8208851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Molecular cloning and expression of anthocyanin biosynthesis genes from 'Fuji apple'." Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fuji. Tissue: Peelings.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69664 mRNA. Translation: CAA49353.1. AF117270 mRNA. Translation: AAD26206.1.
PIR	S31458.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_MALDO

Accession

Primary (citable) accession number: Q06942

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 4, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)