ID   EA6_ARATH               Reviewed;         478 AA.
AC   Q06915;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   24-JAN-2024, entry version 149.
DE   RecName: Full=Probable glucan endo-1,3-beta-glucosidase A6;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Anther-specific protein A6;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   AltName: Full=Protein MATERNAL EFFECT EMBRYO ARREST 48 {ECO:0000303|PubMed:15634699};
DE   Flags: Precursor;
GN   Name=A6 {ECO:0000303|PubMed:8281185};
GN   Synonyms=MEE48 {ECO:0000303|PubMed:15634699};
GN   OrderedLocusNames=At4g14080; ORFNames=dl3080c;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=8281185; DOI=10.1046/j.1365-313x.1993.04061023.x;
RA   Hird D.L., Worrall D., Hodge R., Smartt S., Paul W., Scott R.;
RT   "The anther-specific protein encoded by the Brassica napus and Arabidopsis
RT   thaliana A6 gene displays similarity to beta-1,3-glucanases.";
RL   Plant J. 4:1023-1033(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9461215; DOI=10.1038/35140;
RA   Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA   Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA   Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA   Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA   De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA   Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA   Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA   Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA   Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA   Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA   Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA   Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT   "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT   thaliana.";
RL   Nature 391:485-488(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=15634699; DOI=10.1242/dev.01595;
RA   Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA   Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT   "Genetic and molecular identification of genes required for female
RT   gametophyte development and function in Arabidopsis.";
RL   Development 132:603-614(2005).
RN   [7]
RP   FUNCTION.
RX   PubMed=21849515; DOI=10.1104/pp.111.179523;
RA   Dobritsa A.A., Geanconteri A., Shrestha J., Carlson A., Kooyers N.,
RA   Coerper D., Urbanczyk-Wochniak E., Bench B.J., Sumner L.W., Swanson R.,
RA   Preuss D.;
RT   "A large-scale genetic screen in Arabidopsis to identify genes involved in
RT   pollen exine production.";
RL   Plant Physiol. 157:947-970(2011).
CC   -!- FUNCTION: Probable beta-1,3-glucanase that may be involved in the
CC       degradation of callose walls around the microspore tetrad during pollen
CC       development (Probable). May be required for pollen exine formation
CC       (PubMed:21849515). {ECO:0000269|PubMed:21849515,
CC       ECO:0000305|PubMed:8281185}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- TISSUE SPECIFICITY: Anthers. {ECO:0000269|PubMed:8281185}.
CC   -!- PTM: Contains two additional disulfide bonds, but it is unclear if they
CC       are between the pairs Cys-409-Cys-416 and Cys-425-Cys-471 or between
CC       the pairs Cys-409-Cys-471 and Cys-416-Cys-425.
CC       {ECO:0000250|UniProtKB:Q94G86}.
CC   -!- DISRUPTION PHENOTYPE: Embryonic lethality due to division arrested at
CC       one-cell zygotic stage. {ECO:0000269|PubMed:15634699}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; X70409; CAA49853.1; -; Genomic_DNA.
DR   EMBL; Z97335; CAB10187.1; -; Genomic_DNA.
DR   EMBL; AL161538; CAB78450.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE83368.1; -; Genomic_DNA.
DR   EMBL; AY099580; AAM20432.1; -; mRNA.
DR   EMBL; BT002150; AAN72161.1; -; mRNA.
DR   PIR; S31906; S31906.
DR   RefSeq; NP_193144.1; NM_117483.4.
DR   AlphaFoldDB; Q06915; -.
DR   SMR; Q06915; -.
DR   STRING; 3702.Q06915; -.
DR   CAZy; CBM43; Carbohydrate-Binding Module Family 43.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   PaxDb; 3702-AT4G14080-1; -.
DR   ProteomicsDB; 224708; -.
DR   EnsemblPlants; AT4G14080.1; AT4G14080.1; AT4G14080.
DR   GeneID; 827044; -.
DR   Gramene; AT4G14080.1; AT4G14080.1; AT4G14080.
DR   KEGG; ath:AT4G14080; -.
DR   Araport; AT4G14080; -.
DR   TAIR; AT4G14080; MEE48.
DR   eggNOG; ENOG502QRGZ; Eukaryota.
DR   HOGENOM; CLU_024953_3_4_1; -.
DR   InParanoid; Q06915; -.
DR   OMA; PQTMIRF; -.
DR   OrthoDB; 46602at2759; -.
DR   PhylomeDB; Q06915; -.
DR   BioCyc; ARA:AT4G14080-MONOMER; -.
DR   PRO; PR:Q06915; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q06915; baseline and differential.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR   GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR012946; X8.
DR   PANTHER; PTHR32227:SF406; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE A6-RELATED; 1.
DR   PANTHER; PTHR32227; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   Pfam; PF07983; X8; 1.
DR   SMART; SM00768; X8; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
DR   Genevisible; Q06915; AT.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycosidase; Hydrolase; Plant defense; Reference proteome;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..478
FT                   /note="Probable glucan endo-1,3-beta-glucosidase A6"
FT                   /id="PRO_0000011883"
FT   ACT_SITE        135
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        280
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   DISULFID        390..453
FT                   /evidence="ECO:0000250|UniProtKB:Q94G86"
SQ   SEQUENCE   478 AA;  53472 MW;  68EAE92974E8FA05 CRC64;
     MSLLAFFLFT ILVFSSSCCS ATRFQGHRYM QRKTMLDLAS KIGINYGRRG NNLPSPYQSI
     NFIKSIKAGH VKLYDADPES LTLLSQTNLY VTITVPNHQI TALSSNQTIA DEWVRTNILP
     YYPQTQIRFV LVGNEILSYN SGNVSVNLVP AMRKIVNSLR LHGIHNIKVG TPLAMDSLRS
     SFPPSNGTFR EEITGPVMLP LLKFLNGTNS YFFLNVHPYF RWSRNPMNTS LDFALFQGHS
     TYTDPQTGLV YRNLLDQMLD SVLFAMTKLG YPHMRLAISE TGWPNFGDID ETGANILNAA
     TYNRNLIKKM SASPPIGTPS RPGLPIPTFV FSLFNENQKS GSGTQRHWGI LHPDGSPIYD
     VDFTGQTPLT GFNPLPKPTN NVPYKGQVWC VPVEGANETE LEETLRMACA QSNTTCAALA
     PGRECYEPVS IYWHASYALN SYWAQFRNQS IQCFFNGLAH ETTTNPGNDR CKFPSVTL
//