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Protein

Adaptive-response sensory-kinase SasA

Gene

sasA

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in signal transduction. Participates in the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria, via its interaction with KaiC. Required for robustness of the circadian rhythm of gene expression and is involved in clock outputs.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Biological rhythms, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_2114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptive-response sensory-kinase SasA (EC:2.7.13.3)
Alternative name(s):
Synechococcus adaptive sensor protein A
Gene namesi
Name:sasA
Synonyms:sarS
Ordered Locus Names:Synpcc7942_2114
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1611H → Q: Lowers the amplitude of circadian rhythm. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Adaptive-response sensory-kinase SasAPRO_0000074872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei161 – 1611Phosphohistidine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with KaiC. Participates in the KaiABC complex, whose core is composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
kaiCQ79PF46EBI-626872,EBI-592287

Protein-protein interaction databases

IntActiQ06904. 3 interactions.
STRINGi1140.Synpcc7942_2114.

Structurei

Secondary structure

1
387
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 207Combined sources
Helixi24 – 4017Combined sources
Beta strandi46 – 527Combined sources
Turni53 – 553Combined sources
Helixi57 – 626Combined sources
Beta strandi67 – 7812Combined sources
Beta strandi80 – 856Combined sources
Helixi87 – 9913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T4YNMR-A4-103[»]
1T4ZNMR-A4-103[»]
ProteinModelPortaliQ06904.
SMRiQ06904. Positions 2-106.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06904.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 382225Histidine kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9797Interaction with KaiCAdd
BLAST

Domaini

Although the N-terminal domain, which mediates the interaction with KaiC, shares some sequence similarity with KaiB, it is not structurally related to KaiB.

Sequence similaritiesi

Contains 1 histidine kinase domain.Curated

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000233315.
KOiK08479.
OrthoDBiPOG091H0M9D.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.30.10. 1 hit.
HAMAPiMF_01837. Kinase_SasA. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011649. KaiB_domain.
IPR023527. Kinase_SasA.
IPR004358. Sig_transdc_His_kin-like_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF07689. KaiB. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM01248. KaiB. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGESLSPQAL AQPLLLQLFV DTRPLSQHIV QRVKNILAAV EATVPISLQV
60 70 80 90 100
INVADQPQLV EYYRLVVTPA LVKIGPGSRQ VLSGIDLTDQ LANQLPQWLV
110 120 130 140 150
QQEAFFADRE PPEVNIPFTE LGQPETPALQ QADAFFQLQQ QYADLSERTK
160 170 180 190 200
FLEQVIALVA HDLRNPLTAA LLAVDTIQIR SQSFSVATAK EMQGLCSLFD
210 220 230 240 250
QARSQLREIE RMIAEILEAT RHSGESLRIN PREVVFEPLL QQVLEQLHER
260 270 280 290 300
WRSKQQQLIT DVPGDLPTLY ADPDRLRQVL VNLLDNAIKY TPPGGTITIA
310 320 330 340 350
ALHRTSQKVQ ISISDTGSGI PRDQLSVIFK NLVRLSRDSS QEGYGIGLSV
360 370 380
CQRIVQAHFG RIWVASELGQ GSTFHFTMPV YRYTMPC
Length:387
Mass (Da):43,315
Last modified:April 18, 2006 - v2
Checksum:i04A0A097C3EE0438
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1351F → L in BAA03145 (PubMed:8370532).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14056 Genomic DNA. Translation: BAA03145.1.
CP000100 Genomic DNA. Translation: ABB58144.1.
PIRiJN0793.
RefSeqiWP_011378322.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB58144; ABB58144; Synpcc7942_2114.
KEGGisyf:Synpcc7942_2114.
PATRICi23789717. VBISynElo51371_2380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14056 Genomic DNA. Translation: BAA03145.1.
CP000100 Genomic DNA. Translation: ABB58144.1.
PIRiJN0793.
RefSeqiWP_011378322.1. NC_007604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T4YNMR-A4-103[»]
1T4ZNMR-A4-103[»]
ProteinModelPortaliQ06904.
SMRiQ06904. Positions 2-106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ06904. 3 interactions.
STRINGi1140.Synpcc7942_2114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB58144; ABB58144; Synpcc7942_2114.
KEGGisyf:Synpcc7942_2114.
PATRICi23789717. VBISynElo51371_2380.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000233315.
KOiK08479.
OrthoDBiPOG091H0M9D.

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_2114-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06904.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.30.10. 1 hit.
HAMAPiMF_01837. Kinase_SasA. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011649. KaiB_domain.
IPR023527. Kinase_SasA.
IPR004358. Sig_transdc_His_kin-like_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF07689. KaiB. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM01248. KaiB. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSASA_SYNE7
AccessioniPrimary (citable) accession number: Q06904
Secondary accession number(s): Q31LC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 18, 2006
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.