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Protein

Prolyl endopeptidase

Gene
N/A
Organism
Aeromonas hydrophila
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei538 – 5381Charge relay systemPROSITE-ProRule annotation
Active sitei657 – 6571Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type exopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidase (EC:3.4.21.26)
Short name:
PE
Alternative name(s):
Post-proline cleaving enzyme
OrganismiAeromonas hydrophila
Taxonomic identifieri644 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 690689Prolyl endopeptidasePRO_0000122399Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ06903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Curated

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKARLHYP VTRQSEQLDH YFGQAVADPY RWLEDDRSPE TEAWVKAQNR
60 70 80 90 100
VTQDYLAQIP FRDAIKGKLA TSWNYAKEGA PFREGRYHYF FKNDGLQNQN
110 120 130 140 150
VLCGQLAGKP AEVFLDPNLL SPDGTTALDQ LSFSRDGKTL AYSLSLAGSD
160 170 180 190 200
WREIHLMDVE SKQPLETPLR DVKFSGISWL GNEGFFYSSY DKPDGSELSA
210 220 230 240 250
RTDQHKLYFH RLGTAQEEDR LVFGAIPAQR HRYVGATVTE DDRYLLISAA
260 270 280 290 300
DSTSGNRLYV KDLTREGAPL LTVQGDLAAD VSLVDNKGSR LYLLTNRDAP
310 320 330 340 350
NRRLVTVEAD NPGPEQWRDL IPERQQVLTV HSGGGYLFAE YMVDATARVE
360 370 380 390 400
QFDHDGKRVR EVGLPGLGSV SGFNGKQDDP ALYFGFENYA QPPTLYKFEP
410 420 430 440 450
NSGAISLYRA SAAPFKPEDY VSEQRFYRSK DGTRVPLIIS YRKGLKLDGS
460 470 480 490 500
NPTILYGYGG FDVSLTPSFS VSVANWLDLG GVYAVANLRG GGEYGQAWHL
510 520 530 540 550
AGTRMNKQNV FDDFIAAAEY LKAEGYTRTD RLAIRGGSNG GLLVGAVMTQ
560 570 580 590 600
RPDLMRVACQ AVGVLDMLRY HTFTAGAGWA YDYGTSADSE AMFDYLKGYS
610 620 630 640 650
PLHSVRAGVS YPSTLVTTAD HDDRVVPAHS FKFAATLQAD DAGPHPQLIR
660 670 680 690
IETNAGHGAG TPVAKLIEQS ADIYAFTLFE MGYRQLPRQP
Length:690
Mass (Da):76,515
Last modified:January 23, 2007 - v2
Checksum:i854677C4AE753EB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14005 Genomic DNA. Translation: BAA03105.1.
PIRiJN0585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14005 Genomic DNA. Translation: BAA03105.1.
PIRiJN0585.

3D structure databases

ProteinModelPortaliQ06903.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prolyl endopeptidase from Aeromonas hydrophila: cloning, sequencing, and expression of the enzyme gene, and characterization of the expressed enzyme."
    Kanatani A., Yoshimoto T., Kitazono A., Kokubo T., Tsuru D.
    J. Biochem. 113:790-796(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: AK-9.

Entry informationi

Entry nameiPPCE_AERHY
AccessioniPrimary (citable) accession number: Q06903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.