Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspergillopepsin-1

Gene

pepA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A. Hydrolyzes bovine chymotrysinogen A between positions 'Arg-15' and 'Ile-16'.1 Publication

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.1 Publication

Enzyme regulationi

Inhibited by sodium lauryl sulfonate.1 Publication

Kineticsi

kcat is 1.65 sec(-1) with Z-His-Phe-Phe-OEt as substrate, 0.35 sec(-1) with Z-Ala-Ala-Phe-Phe-OPy4Pr as substrate, 13 sec(-1) with bovine trypsinogen as substrate and 1.14 sec(-1) with bovine chymotrysinogen A as substrate.1 Publication
  1. KM=0.064 mM for Z-His-Phe-Phe-OEt1 Publication
  2. KM=0.037 mM for Z-Ala-Ala-Phe-Phe-OPy4Pr1 Publication
  3. KM=0.1 mM for bovine trypsinogen1 Publication
  4. KM=0.18 mM for bovine chymotrysinogen A1 Publication

    pH dependencei

    Optimum pH is 3.0-4.2.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei111PROSITE-ProRule annotation1
    Active sitei293PROSITE-ProRule annotation1

    GO - Molecular functioni

    Keywordsi

    Molecular functionAspartyl protease, Hydrolase, Protease

    Enzyme and pathway databases

    BRENDAi3.4.23.18. 522.

    Protein family/group databases

    MEROPSiA01.026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspergillopepsin-1Curated (EC:3.4.23.181 Publication)
    Alternative name(s):
    Acid protease A21 Publication
    Aspartic protease pepA
    Aspergillopepsin I
    Aspergillopepsin O1 Publication
    Short name:
    PEPO
    Aspergillopeptidase A
    Short name:
    PEPA
    Gene namesi
    Name:pepA1 Publication
    Synonyms:pepO1 Publication
    ORF Names:AO090120000474
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    Proteomesi
    • UP000006564 Componenti: Chromosome 5

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 20Sequence analysisAdd BLAST20
    PropeptideiPRO_000002592221 – 77Activation peptideBy similarityAdd BLAST57
    ChainiPRO_000002592378 – 404Aspergillopepsin-1Add BLAST327

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi140N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Disulfide bondi329 ↔ 364PROSITE-ProRule annotation

    Post-translational modificationi

    Two isozymes of this enzyme exist which differ only by their non-covalent association with carbohydrate.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ06902.

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00011357.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06902.
    SMRiQ06902.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini95 – 401Peptidase A1PROSITE-ProRule annotationAdd BLAST307

    Sequence similaritiesi

    Belongs to the peptidase A1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000167484.
    KOiK06004.
    OMAiFGYQPVD.
    OrthoDBiEOG092C49YE.

    Family and domain databases

    CDDicd06097. Aspergillopepsin_like. 1 hit.
    Gene3Di2.40.70.10. 2 hits.
    InterProiView protein in InterPro
    IPR001461. Aspartic_peptidase_A1.
    IPR001969. Aspartic_peptidase_AS.
    IPR034163. Aspergillopepsin-like_cat_dom.
    IPR033121. PEPTIDASE_A1.
    IPR021109. Peptidase_aspartic_dom.
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiView protein in Pfam
    PF00026. Asp. 1 hit.
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiView protein in PROSITE
    PS00141. ASP_PROTEASE. 2 hits.
    PS51767. PEPTIDASE_A1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06902-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVILSKVAAV AVGLSTVASA LPTGPSHSPH ARRGFTINQI TRQTARVGPK
    60 70 80 90 100
    TASFPAIYSR ALAKYGGTVP AHLKSAVASG HGTVVTSPEP NDIEYLTPVN
    110 120 130 140 150
    IGGTTLNLDF DTGSADLWVF SEELPKSEQT GHDVYKPSGN ASKIAGASWD
    160 170 180 190 200
    ISYGDGSSAS GDVYQDTVTV GGVTAQGQAV EAASKISDQF VQDKNNDGLL
    210 220 230 240 250
    GLAFSSINTV KPKPQTTFFD TVKDQLDAPL FAVTLKYHAP GSYDFGFIDK
    260 270 280 290 300
    SKFTGELAYA DVDDSQGFWQ FTADGYSVGK GDAQKAPITG IADTGTTLVM
    310 320 330 340 350
    LDDEIVDAYY KQVQGAKNDA SAGGYVFPCE TELPEFTVVI GSYNAVIPGK
    360 370 380 390 400
    HINYAPLQEG SSTCVGGIQS NSGLGLSILG DVFLKSQYVV FDSQGPRLGF

    AAQA
    Length:404
    Mass (Da):42,313
    Last modified:May 2, 2006 - v2
    Checksum:iBFC21C29EC0A24DF
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti289T → S in BAA02994 (PubMed:7763981).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D13894 Genomic DNA. Translation: BAA02994.1.
    M92927 Genomic DNA. Translation: AAA32704.1.
    AP007166 Genomic DNA. Translation: BAE63042.1.
    PIRiJN0630.
    RefSeqiXP_001824175.1. XM_001824123.2.

    Genome annotation databases

    EnsemblFungiiBAE63042; BAE63042; AO090120000474.
    GeneIDi5996434.
    KEGGiaor:AOR_1_830094.

    Similar proteinsi

    Entry informationi

    Entry nameiPEPA_ASPOR
    AccessioniPrimary (citable) accession number: Q06902
    Secondary accession number(s): Q00249, Q2U5X3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 2, 2006
    Last modified: July 5, 2017
    This is version 119 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families