Reviewed, UniProtKB/Swiss-Prot Q06892 (POS5_YEAST)

Names and origin

Protein names

Recommended name:
    NADH kinase POS5, mitochondrial
    EC=2.7.1.86

Gene names

Name:	POS5
Ordered Locus Names:	YPL188W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates both NADH and NAD+, with a twofold preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH. Ref.4 Ref.5
Catalytic activity	ATP + NADH = ADP + NADPH.
Subcellular location	Mitochondrion matrix. Ref.4 Ref.5 Ref.6
Miscellaneous	Present with 4650 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the NAD kinase family.
Biophysicochemical properties	Kinetic parameters: KM=105 µM for NADH KM=2.1 mM for ATP pH dependence: Optimum pH is 8.5.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP biosynthetic process Ref.4 Inferred from direct assay. Source: SGD response to oxidative stress Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Ref.4 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: InterPro NADH kinase activity Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 414		NADH kinase POS5, mitochondrial		PRO_0000120715
Experimental info
	Sequence conflict	37 – 38	2	KP → EA in CAA59017. Ref.1
	Sequence conflict	180	1	S → L in CAA59017. Ref.1
	Sequence conflict	329	1	V → D in CAA59017. Ref.1
	Sequence conflict	343	1	I → S in CAA59017. Ref.1
	Sequence conflict	398 – 401	4	LGFN → CRIH in CAA59017. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q06892-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 002CFC271A67B557	FASTA	414	46,247
	10 20 30 40 50 60 MFVRVKLNKP VKWYRFYSTL DSHSLKLQSG SKFVKIKPVN NLRSSSSADF VSPPNSKLQS 70 80 90 100 110 120 LIWQNPLQNV YITKKPWTPS TREAMVEFIT HLHESYPEVN VIVQPDVAEE ISQDFKSPLE 130 140 150 160 170 180 NDPNRPHILY TGPEQDIVNR TDLLVTLGGD GTILHGVSMF GNTQVPPVLA FALGTLGFLS 190 200 210 220 230 240 PFDFKEHKKV FQEVISSRAK CLHRTRLECH LKKKDSNSSI VTHAMNDIFL HRGNSPHLTN 250 260 270 280 290 300 LDIFIDGEFL TRTTADGVAL ATPTGSTAYS LSAGGSIVSP LVPAILMTPI CPRSLSFRPL 310 320 330 340 350 360 ILPHSSHIRI KIGSKLNQKP VNSVVKLSVD GIPQQDLDVG DEIYVINEVG TIYIDGTQLP 370 380 390 400 410 TTRKTENDFN NSKKPKRSGI YCVAKTENDW IRGINELLGF NSSFRLTKRQ TDND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Gruenbein R., Krems B., Entian K.-D. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Characterization of NADH kinase from Saccharomyces cerevisiae." Iwahashi Y., Hitoshio A., Tajima N., Nakamura T. J. Biochem. 105:588-593(1989) [PubMed: 2547755] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"A novel NADH kinase is the mitochondrial source of NADPH in Saccharomyces cerevisiae." Outten C.E., Culotta V.C. EMBO J. 22:2015-2024(2003) [PubMed: 12727869] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"POS5 gene of Saccharomyces cerevisiae encodes a mitochondrial NADH kinase required for stability of mitochondrial DNA." Strand M.K., Stuart G.R., Longley M.J., Graziewicz M.A., Dominick O.C., Copeland W.C. Eukaryot. Cell 2:809-820(2003) [PubMed: 12912900] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84260 Genomic DNA. Translation: CAA59017.1. Z73544 Genomic DNA. Translation: CAA97900.1.
PIR	S65200.
RefSeq	NP_015136.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5503N.
IntAct	Q06892. 6 interactions.
Genome annotation databases
Ensembl	YPL188W. Saccharomyces cerevisiae. [Contig view]
GeneID	855913.
GenomeReviews	Gene locus YPL188W in contig U00094_GR.
KEGG	sce:YPL188W.
NMPDR	fig|4932.3.peg.6265.
Organism-specific databases
CYGD	YPL188w.
SGD	S000006109. POS5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06892.
OMA	Q06892. GFNSSFR.
Enzyme and pathway databases
BRENDA	2.7.1.86. 250.
Gene expression databases
GermOnline	YPL188W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. ATP_NADK. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980625.

Entry information

Entry name

POS5_YEAST

Accession

Primary (citable) accession number: Q06892
Secondary accession number(s): Q08928

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)