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Protein

NADH kinase POS5, mitochondrial

Gene

POS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates both NADH and NAD+, with a twofold preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH.2 Publications

Catalytic activityi

ATP + NADH = ADP + NADPH.

Kineticsi

  1. KM=105 µM for NADH1 Publication
  2. KM=2.1 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • NAD+ kinase activity Source: InterPro
    • NADH kinase activity Source: SGD

    GO - Biological processi

    • cellular response to oxidative stress Source: SGD
    • NAD metabolic process Source: InterPro
    • NADP biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34081-MONOMER.
    YEAST:G3O-34081-MONOMER.
    BRENDAi2.7.1.23. 984.
    2.7.1.86. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH kinase POS5, mitochondrial (EC:2.7.1.86)
    Gene namesi
    Name:POS5
    Ordered Locus Names:YPL188W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPL188W.
    SGDiS000006109. POS5.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrial matrix Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000120715? – 414NADH kinase POS5, mitochondrial
    Transit peptidei1 – ?MitochondrionSequence analysis

    Proteomic databases

    MaxQBiQ06892.
    PRIDEiQ06892.

    Interactioni

    Protein-protein interaction databases

    BioGridi35995. 48 interactors.
    DIPiDIP-5503N.
    IntActiQ06892. 3 interactors.
    MINTiMINT-569331.

    Structurei

    Secondary structure

    1414
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi34 – 38Combined sources5
    Helixi39 – 41Combined sources3
    Beta strandi49 – 51Combined sources3
    Beta strandi59 – 62Combined sources4
    Beta strandi69 – 74Combined sources6
    Helixi79 – 95Combined sources17
    Beta strandi100 – 102Combined sources3
    Helixi105 – 112Combined sources8
    Helixi119 – 121Combined sources3
    Beta strandi127 – 131Combined sources5
    Helixi134 – 140Combined sources7
    Beta strandi142 – 149Combined sources8
    Helixi150 – 158Combined sources9
    Turni159 – 162Combined sources4
    Beta strandi168 – 172Combined sources5
    Helixi184 – 186Combined sources3
    Helixi187 – 195Combined sources9
    Beta strandi200 – 204Combined sources5
    Beta strandi207 – 212Combined sources6
    Beta strandi220 – 231Combined sources12
    Beta strandi239 – 245Combined sources7
    Beta strandi248 – 261Combined sources14
    Helixi264 – 267Combined sources4
    Helixi269 – 272Combined sources4
    Beta strandi284 – 293Combined sources10
    Beta strandi300 – 303Combined sources4
    Beta strandi308 – 312Combined sources5
    Beta strandi321 – 323Combined sources3
    Beta strandi325 – 329Combined sources5
    Beta strandi332 – 337Combined sources6
    Beta strandi342 – 349Combined sources8
    Beta strandi379 – 383Combined sources5
    Helixi389 – 396Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3AFOX-ray2.00A/B27-414[»]
    ProteinModelPortaliQ06892.
    SMRiQ06892.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000172029.
    InParanoidiQ06892.
    KOiK19386.
    OMAiGVPCVIR.
    OrthoDBiEOG092C2DOX.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 2 hits.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06892-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFVRVKLNKP VKWYRFYSTL DSHSLKLQSG SKFVKIKPVN NLRSSSSADF
    60 70 80 90 100
    VSPPNSKLQS LIWQNPLQNV YITKKPWTPS TREAMVEFIT HLHESYPEVN
    110 120 130 140 150
    VIVQPDVAEE ISQDFKSPLE NDPNRPHILY TGPEQDIVNR TDLLVTLGGD
    160 170 180 190 200
    GTILHGVSMF GNTQVPPVLA FALGTLGFLS PFDFKEHKKV FQEVISSRAK
    210 220 230 240 250
    CLHRTRLECH LKKKDSNSSI VTHAMNDIFL HRGNSPHLTN LDIFIDGEFL
    260 270 280 290 300
    TRTTADGVAL ATPTGSTAYS LSAGGSIVSP LVPAILMTPI CPRSLSFRPL
    310 320 330 340 350
    ILPHSSHIRI KIGSKLNQKP VNSVVKLSVD GIPQQDLDVG DEIYVINEVG
    360 370 380 390 400
    TIYIDGTQLP TTRKTENDFN NSKKPKRSGI YCVAKTENDW IRGINELLGF
    410
    NSSFRLTKRQ TDND
    Length:414
    Mass (Da):46,247
    Last modified:December 1, 2000 - v2
    Checksum:i002CFC271A67B557
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti37 – 38KP → EA in CAA59017 (Ref. 1) Curated2
    Sequence conflicti180S → L in CAA59017 (Ref. 1) Curated1
    Sequence conflicti329V → D in CAA59017 (Ref. 1) Curated1
    Sequence conflicti343I → S in CAA59017 (Ref. 1) Curated1
    Sequence conflicti398 – 401LGFN → CRIH in CAA59017 (Ref. 1) Curated4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X84260 Genomic DNA. Translation: CAA59017.1.
    Z73544 Genomic DNA. Translation: CAA97900.1.
    BK006949 Genomic DNA. Translation: DAA11247.1.
    PIRiS65200.
    RefSeqiNP_015136.1. NM_001184002.1.

    Genome annotation databases

    EnsemblFungiiYPL188W; YPL188W; YPL188W.
    GeneIDi855913.
    KEGGisce:YPL188W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X84260 Genomic DNA. Translation: CAA59017.1.
    Z73544 Genomic DNA. Translation: CAA97900.1.
    BK006949 Genomic DNA. Translation: DAA11247.1.
    PIRiS65200.
    RefSeqiNP_015136.1. NM_001184002.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3AFOX-ray2.00A/B27-414[»]
    ProteinModelPortaliQ06892.
    SMRiQ06892.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35995. 48 interactors.
    DIPiDIP-5503N.
    IntActiQ06892. 3 interactors.
    MINTiMINT-569331.

    Proteomic databases

    MaxQBiQ06892.
    PRIDEiQ06892.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYPL188W; YPL188W; YPL188W.
    GeneIDi855913.
    KEGGisce:YPL188W.

    Organism-specific databases

    EuPathDBiFungiDB:YPL188W.
    SGDiS000006109. POS5.

    Phylogenomic databases

    HOGENOMiHOG000172029.
    InParanoidiQ06892.
    KOiK19386.
    OMAiGVPCVIR.
    OrthoDBiEOG092C2DOX.

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34081-MONOMER.
    YEAST:G3O-34081-MONOMER.
    BRENDAi2.7.1.23. 984.
    2.7.1.86. 984.

    Miscellaneous databases

    PROiQ06892.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 2 hits.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPOS5_YEAST
    AccessioniPrimary (citable) accession number: Q06892
    Secondary accession number(s): D6W3I1, Q08928
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: November 2, 2016
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.