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Protein

NADH kinase POS5, mitochondrial

Gene

POS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates both NADH and NAD+, with a twofold preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH.2 Publications

Catalytic activityi

ATP + NADH = ADP + NADPH.

Kineticsi

  1. KM=105 µM for NADH1 Publication
  2. KM=2.1 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • NAD+ kinase activity Source: InterPro
    • NADH kinase activity Source: SGD

    GO - Biological processi

    • cellular response to oxidative stress Source: SGD
    • NAD metabolic process Source: InterPro
    • NADP biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34081-MONOMER.
    YEAST:G3O-34081-MONOMER.
    BRENDAi2.7.1.23. 984.
    2.7.1.86. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH kinase POS5, mitochondrial (EC:2.7.1.86)
    Gene namesi
    Name:POS5
    Ordered Locus Names:YPL188W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPL188W.
    SGDiS000006109. POS5.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrial matrix Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 414NADH kinase POS5, mitochondrialPRO_0000120715
    Transit peptidei1 – ?MitochondrionSequence analysis

    Proteomic databases

    MaxQBiQ06892.

    Interactioni

    Protein-protein interaction databases

    BioGridi35995. 48 interactions.
    DIPiDIP-5503N.
    IntActiQ06892. 3 interactions.
    MINTiMINT-569331.

    Structurei

    Secondary structure

    1
    414
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 385Combined sources
    Helixi39 – 413Combined sources
    Beta strandi49 – 513Combined sources
    Beta strandi59 – 624Combined sources
    Beta strandi69 – 746Combined sources
    Helixi79 – 9517Combined sources
    Beta strandi100 – 1023Combined sources
    Helixi105 – 1128Combined sources
    Helixi119 – 1213Combined sources
    Beta strandi127 – 1315Combined sources
    Helixi134 – 1407Combined sources
    Beta strandi142 – 1498Combined sources
    Helixi150 – 1589Combined sources
    Turni159 – 1624Combined sources
    Beta strandi168 – 1725Combined sources
    Helixi184 – 1863Combined sources
    Helixi187 – 1959Combined sources
    Beta strandi200 – 2045Combined sources
    Beta strandi207 – 2126Combined sources
    Beta strandi220 – 23112Combined sources
    Beta strandi239 – 2457Combined sources
    Beta strandi248 – 26114Combined sources
    Helixi264 – 2674Combined sources
    Helixi269 – 2724Combined sources
    Beta strandi284 – 29310Combined sources
    Beta strandi300 – 3034Combined sources
    Beta strandi308 – 3125Combined sources
    Beta strandi321 – 3233Combined sources
    Beta strandi325 – 3295Combined sources
    Beta strandi332 – 3376Combined sources
    Beta strandi342 – 3498Combined sources
    Beta strandi379 – 3835Combined sources
    Helixi389 – 3968Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AFOX-ray2.00A/B27-414[»]
    ProteinModelPortaliQ06892.
    SMRiQ06892. Positions 27-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000172029.
    InParanoidiQ06892.
    KOiK19386.
    OMAiGVPCVIR.
    OrthoDBiEOG092C2DOX.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 2 hits.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06892-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFVRVKLNKP VKWYRFYSTL DSHSLKLQSG SKFVKIKPVN NLRSSSSADF
    60 70 80 90 100
    VSPPNSKLQS LIWQNPLQNV YITKKPWTPS TREAMVEFIT HLHESYPEVN
    110 120 130 140 150
    VIVQPDVAEE ISQDFKSPLE NDPNRPHILY TGPEQDIVNR TDLLVTLGGD
    160 170 180 190 200
    GTILHGVSMF GNTQVPPVLA FALGTLGFLS PFDFKEHKKV FQEVISSRAK
    210 220 230 240 250
    CLHRTRLECH LKKKDSNSSI VTHAMNDIFL HRGNSPHLTN LDIFIDGEFL
    260 270 280 290 300
    TRTTADGVAL ATPTGSTAYS LSAGGSIVSP LVPAILMTPI CPRSLSFRPL
    310 320 330 340 350
    ILPHSSHIRI KIGSKLNQKP VNSVVKLSVD GIPQQDLDVG DEIYVINEVG
    360 370 380 390 400
    TIYIDGTQLP TTRKTENDFN NSKKPKRSGI YCVAKTENDW IRGINELLGF
    410
    NSSFRLTKRQ TDND
    Length:414
    Mass (Da):46,247
    Last modified:December 1, 2000 - v2
    Checksum:i002CFC271A67B557
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 382KP → EA in CAA59017 (Ref. 1) Curated
    Sequence conflicti180 – 1801S → L in CAA59017 (Ref. 1) Curated
    Sequence conflicti329 – 3291V → D in CAA59017 (Ref. 1) Curated
    Sequence conflicti343 – 3431I → S in CAA59017 (Ref. 1) Curated
    Sequence conflicti398 – 4014LGFN → CRIH in CAA59017 (Ref. 1) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X84260 Genomic DNA. Translation: CAA59017.1.
    Z73544 Genomic DNA. Translation: CAA97900.1.
    BK006949 Genomic DNA. Translation: DAA11247.1.
    PIRiS65200.
    RefSeqiNP_015136.1. NM_001184002.1.

    Genome annotation databases

    EnsemblFungiiYPL188W; YPL188W; YPL188W.
    GeneIDi855913.
    KEGGisce:YPL188W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X84260 Genomic DNA. Translation: CAA59017.1.
    Z73544 Genomic DNA. Translation: CAA97900.1.
    BK006949 Genomic DNA. Translation: DAA11247.1.
    PIRiS65200.
    RefSeqiNP_015136.1. NM_001184002.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AFOX-ray2.00A/B27-414[»]
    ProteinModelPortaliQ06892.
    SMRiQ06892. Positions 27-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35995. 48 interactions.
    DIPiDIP-5503N.
    IntActiQ06892. 3 interactions.
    MINTiMINT-569331.

    Proteomic databases

    MaxQBiQ06892.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYPL188W; YPL188W; YPL188W.
    GeneIDi855913.
    KEGGisce:YPL188W.

    Organism-specific databases

    EuPathDBiFungiDB:YPL188W.
    SGDiS000006109. POS5.

    Phylogenomic databases

    HOGENOMiHOG000172029.
    InParanoidiQ06892.
    KOiK19386.
    OMAiGVPCVIR.
    OrthoDBiEOG092C2DOX.

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-34081-MONOMER.
    YEAST:G3O-34081-MONOMER.
    BRENDAi2.7.1.23. 984.
    2.7.1.86. 984.

    Miscellaneous databases

    PROiQ06892.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 2 hits.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPOS5_YEAST
    AccessioniPrimary (citable) accession number: Q06892
    Secondary accession number(s): D6W3I1, Q08928
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: September 7, 2016
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.