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Q06891

- RAF_YEAST

UniProt

Q06891 - RAF_YEAST

Protein

Trans-acting factor D

Gene

RAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.1 Publication

    GO - Biological processi

    1. plasmid maintenance Source: SGD
    2. plasmid partitioning Source: UniProtKB-KW

    Keywords - Biological processi

    Plasmid partition

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trans-acting factor D
    Alternative name(s):
    REP-antagonizing factor
    Recombinase-activating factor
    Short name:
    RAF
    Gene namesi
    Name:RAF1
    Ordered Locus Names:R0030W
    Encoded oniPlasmid 2-micron0 Publication
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Plasmid 2-micron

    Organism-specific databases

    CYGDiR0030w.
    SGDiS000029674. RAF1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181Trans-acting factor DPRO_0000283651Add
    BLAST

    Proteomic databases

    MaxQBiQ06891.

    Expressioni

    Inductioni

    Repressed by the negative regulatory complex REP1-REP2.1 Publication

    Gene expression databases

    GenevestigatoriQ06891.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-518N.
    IntActiQ06891. 4 interactions.
    STRINGi4932.R0030W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06891.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    OrthoDBiEOG7Q8D0F.

    Sequencei

    Sequence statusi: Complete.

    Q06891-1 [UniParc]FASTAAdd to Basket

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    MPYKTAIDCI EELATQCFLS KLTDDDVSTF RRVCSKENDI IKLALRIPRT    50
    IDYTSILRLL YDTLPLRSLS FNEALPLFCY SIDPAQQRQC DLRFYLRDVV 100
    KLARPRKRLE MQKALLQWLP SLLSDVTLQL LNDIRIRFEE IQPNIRQTVL 150
    QIYDRTCYPS LNFEHPNLGV FPETDSIFEP V 181
    Length:181
    Mass (Da):21,290
    Last modified:November 1, 1996 - v1
    Checksum:i260CF848B0A51544
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti33 – 331V → A in strain: ATCC 7754.
    Natural varianti36 – 361K → R in strain: ATCC 7754.
    Natural varianti46 – 516RIPRTI → KVPQTV in strain: ATCC 7754.
    Natural varianti67 – 682RS → KF in strain: ATCC 7754.
    Natural varianti104 – 1041R → K in strain: ATCC 7754.
    Natural varianti116 – 1161L → S in strain: ATCC 7754.
    Natural varianti125 – 1251D → N in strain: ATCC 7754.
    Natural varianti136 – 1361I → T in strain: ATCC 7754.
    Natural varianti137 – 1371R → H in strain: ATCC 4108.
    Natural varianti144 – 1441N → D in strain: ATCC 7754.
    Natural varianti152 – 1576IYDRTC → NYGRTG in strain: ATCC 7754.
    Natural varianti162 – 1621N → D in strain: ATCC 7754.
    Natural varianti171 – 1711F → L in strain: ATCC 7754.
    Natural varianti180 – 1801P → S in strain: ATCC 7754.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59342.1.
    M57897 Genomic DNA. Translation: AAA34955.1.
    M57898 Genomic DNA. Translation: AAA34956.1.
    PIRiJQ1027.
    S22793.
    S22872.
    RefSeqiNP_040490.1. NC_001398.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01347 Genomic DNA. Translation: AAB59342.1 .
    M57897 Genomic DNA. Translation: AAA34955.1 .
    M57898 Genomic DNA. Translation: AAA34956.1 .
    PIRi JQ1027.
    S22793.
    S22872.
    RefSeqi NP_040490.1. NC_001398.1.

    3D structure databases

    ProteinModelPortali Q06891.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-518N.
    IntActi Q06891. 4 interactions.
    STRINGi 4932.R0030W.

    Proteomic databases

    MaxQBi Q06891.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    CYGDi R0030w.
    SGDi S000029674. RAF1.

    Phylogenomic databases

    OrthoDBi EOG7Q8D0F.

    Gene expression databases

    Genevestigatori Q06891.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast plasmid."
      Hartley J.L., Donelson J.E.
      Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A364A D5.
    2. "Sequence diversity of yeast 2 microns RAF gene and its co-evolution with STB and REP1."
      Xiao W., Pelcher L.E., Rank G.H.
      Gene 101:75-80(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 4108 and ATCC 7754.
    3. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
      Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
      EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiRAF_YEAST
    AccessioniPrimary (citable) accession number: Q06891
    Secondary accession number(s): Q06966, Q06977
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3