Q06891 (RAF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-acting factor D

Alternative name(s):
REP-antagonizing factor
Recombinase-activating factor
Short name=RAF

Gene names

Name:	RAF1
Ordered Locus Names:	R0030W

Encoded on

Plasmid 2-micron

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning. Ref.3
Induction	Repressed by the negative regulatory complex REP1-REP2. Ref.3

Ontologies

Keywords
Biological process	Plasmid partition
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological_process	plasmid maintenance Inferred from mutant phenotype Ref.3. Source: SGD plasmid partitioning Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred by curator Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Trans-acting factor D		PRO_0000283651
Natural variations
	Natural variant	33	1	V → A in strain: ATCC 7754.
	Natural variant	36	1	K → R in strain: ATCC 7754.
	Natural variant	46 – 51	6	RIPRTI → KVPQTV in strain: ATCC 7754.
	Natural variant	67 – 68	2	RS → KF in strain: ATCC 7754.
	Natural variant	104	1	R → K in strain: ATCC 7754.
	Natural variant	116	1	L → S in strain: ATCC 7754.
	Natural variant	125	1	D → N in strain: ATCC 7754.
	Natural variant	136	1	I → T in strain: ATCC 7754.
	Natural variant	137	1	R → H in strain: ATCC 4108.
	Natural variant	144	1	N → D in strain: ATCC 7754.
	Natural variant	152 – 157	6	IYDRTC → NYGRTG in strain: ATCC 7754.
	Natural variant	162	1	N → D in strain: ATCC 7754.
	Natural variant	171	1	F → L in strain: ATCC 7754.
	Natural variant	180	1	P → S in strain: ATCC 7754.

Sequences

	Sequence		Length	Mass (Da)
	Q06891 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 260CF848B0A51544	FASTA	181	21,290
	10 20 30 40 50 60 MPYKTAIDCI EELATQCFLS KLTDDDVSTF RRVCSKENDI IKLALRIPRT IDYTSILRLL 70 80 90 100 110 120 YDTLPLRSLS FNEALPLFCY SIDPAQQRQC DLRFYLRDVV KLARPRKRLE MQKALLQWLP 130 140 150 160 170 180 SLLSDVTLQL LNDIRIRFEE IQPNIRQTVL QIYDRTCYPS LNFEHPNLGV FPETDSIFEP V

References

[1]	"Nucleotide sequence of the yeast plasmid." Hartley J.L., Donelson J.E. Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A364A D5.
[2]	"Sequence diversity of yeast 2 microns RAF gene and its co-evolution with STB and REP1." Xiao W., Pelcher L.E., Rank G.H. Gene 101:75-80(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 4108 and ATCC 7754.
[3]	"Antagonistic controls regulate copy number of the yeast 2 micron plasmid." Murray J.A.H., Scarpa M., Rossi N., Cesareni G. EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01347 Genomic DNA. Translation: AAB59342.1. M57897 Genomic DNA. Translation: AAA34955.1. M57898 Genomic DNA. Translation: AAA34956.1.
PIR	JQ1027. S22793. S22872.
RefSeq	NP_040490.1. NC_001398.1.
3D structure databases
ProteinModelPortal	Q06891.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-518N.
IntAct	Q06891. 4 interactions.
STRING	4932.R0030W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
CYGD	R0030w.
SGD	S000029674. RAF1.
Gene expression databases
Genevestigator	Q06891.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RAF_YEAST

Accession

Primary (citable) accession number: Q06891
Secondary accession number(s): Q06966, Q06977

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program