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Q06891

- RAF_YEAST

UniProt

Q06891 - RAF_YEAST

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Protein

Trans-acting factor D

Gene

RAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.1 Publication

GO - Biological processi

  1. plasmid maintenance Source: SGD
  2. plasmid partitioning Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Plasmid partition

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting factor D
Alternative name(s):
REP-antagonizing factor
Recombinase-activating factor
Short name:
RAF
Gene namesi
Name:RAF1
Ordered Locus Names:R0030W
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Plasmid 2-micron

Organism-specific databases

CYGDiR0030w.
SGDiS000029674. RAF1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Trans-acting factor DPRO_0000283651Add
BLAST

Proteomic databases

MaxQBiQ06891.

Expressioni

Inductioni

Repressed by the negative regulatory complex REP1-REP2.1 Publication

Gene expression databases

GenevestigatoriQ06891.

Interactioni

Protein-protein interaction databases

DIPiDIP-518N.
IntActiQ06891. 4 interactions.
STRINGi4932.R0030W.

Structurei

3D structure databases

ProteinModelPortaliQ06891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

OrthoDBiEOG7Q8D0F.

Sequencei

Sequence statusi: Complete.

Q06891 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYKTAIDCI EELATQCFLS KLTDDDVSTF RRVCSKENDI IKLALRIPRT
60 70 80 90 100
IDYTSILRLL YDTLPLRSLS FNEALPLFCY SIDPAQQRQC DLRFYLRDVV
110 120 130 140 150
KLARPRKRLE MQKALLQWLP SLLSDVTLQL LNDIRIRFEE IQPNIRQTVL
160 170 180
QIYDRTCYPS LNFEHPNLGV FPETDSIFEP V
Length:181
Mass (Da):21,290
Last modified:November 1, 1996 - v1
Checksum:i260CF848B0A51544
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331V → A in strain: ATCC 7754.
Natural varianti36 – 361K → R in strain: ATCC 7754.
Natural varianti46 – 516RIPRTI → KVPQTV in strain: ATCC 7754.
Natural varianti67 – 682RS → KF in strain: ATCC 7754.
Natural varianti104 – 1041R → K in strain: ATCC 7754.
Natural varianti116 – 1161L → S in strain: ATCC 7754.
Natural varianti125 – 1251D → N in strain: ATCC 7754.
Natural varianti136 – 1361I → T in strain: ATCC 7754.
Natural varianti137 – 1371R → H in strain: ATCC 4108.
Natural varianti144 – 1441N → D in strain: ATCC 7754.
Natural varianti152 – 1576IYDRTC → NYGRTG in strain: ATCC 7754.
Natural varianti162 – 1621N → D in strain: ATCC 7754.
Natural varianti171 – 1711F → L in strain: ATCC 7754.
Natural varianti180 – 1801P → S in strain: ATCC 7754.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59342.1.
M57897 Genomic DNA. Translation: AAA34955.1.
M57898 Genomic DNA. Translation: AAA34956.1.
PIRiJQ1027.
S22793.
S22872.
RefSeqiNP_040490.1. NC_001398.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01347 Genomic DNA. Translation: AAB59342.1 .
M57897 Genomic DNA. Translation: AAA34955.1 .
M57898 Genomic DNA. Translation: AAA34956.1 .
PIRi JQ1027.
S22793.
S22872.
RefSeqi NP_040490.1. NC_001398.1.

3D structure databases

ProteinModelPortali Q06891.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-518N.
IntActi Q06891. 4 interactions.
STRINGi 4932.R0030W.

Proteomic databases

MaxQBi Q06891.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

CYGDi R0030w.
SGDi S000029674. RAF1.

Phylogenomic databases

OrthoDBi EOG7Q8D0F.

Gene expression databases

Genevestigatori Q06891.

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the yeast plasmid."
    Hartley J.L., Donelson J.E.
    Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A364A D5.
  2. "Sequence diversity of yeast 2 microns RAF gene and its co-evolution with STB and REP1."
    Xiao W., Pelcher L.E., Rank G.H.
    Gene 101:75-80(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 4108 and ATCC 7754.
  3. "Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
    Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
    EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiRAF_YEAST
AccessioniPrimary (citable) accession number: Q06891
Secondary accession number(s): Q06966, Q06977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3