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Q06891 (RAF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trans-acting factor D
Alternative name(s):
REP-antagonizing factor
Recombinase-activating factor
Short name=RAF
Gene names
Name:RAF1
Ordered Locus Names:R0030W
Encoded onPlasmid 2-micron
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning. Ref.3

Induction

Repressed by the negative regulatory complex REP1-REP2. Ref.3

Ontologies

Keywords
   Biological processPlasmid partition
   Technical termComplete proteome
Plasmid
Reference proteome
Gene Ontology (GO)
   Biological_processplasmid maintenance

Inferred from mutant phenotype Ref.3. Source: SGD

plasmid partitioning

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred by curator Ref.3. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Trans-acting factor D
PRO_0000283651

Natural variations

Natural variant331V → A in strain: ATCC 7754.
Natural variant361K → R in strain: ATCC 7754.
Natural variant46 – 516RIPRTI → KVPQTV in strain: ATCC 7754.
Natural variant67 – 682RS → KF in strain: ATCC 7754.
Natural variant1041R → K in strain: ATCC 7754.
Natural variant1161L → S in strain: ATCC 7754.
Natural variant1251D → N in strain: ATCC 7754.
Natural variant1361I → T in strain: ATCC 7754.
Natural variant1371R → H in strain: ATCC 4108.
Natural variant1441N → D in strain: ATCC 7754.
Natural variant152 – 1576IYDRTC → NYGRTG in strain: ATCC 7754.
Natural variant1621N → D in strain: ATCC 7754.
Natural variant1711F → L in strain: ATCC 7754.
Natural variant1801P → S in strain: ATCC 7754.

Sequences

Sequence LengthMass (Da)Tools
Q06891 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 260CF848B0A51544

FASTA18121,290
        10         20         30         40         50         60 
MPYKTAIDCI EELATQCFLS KLTDDDVSTF RRVCSKENDI IKLALRIPRT IDYTSILRLL 

        70         80         90        100        110        120 
YDTLPLRSLS FNEALPLFCY SIDPAQQRQC DLRFYLRDVV KLARPRKRLE MQKALLQWLP 

       130        140        150        160        170        180 
SLLSDVTLQL LNDIRIRFEE IQPNIRQTVL QIYDRTCYPS LNFEHPNLGV FPETDSIFEP 


V 

« Hide

References

[1]"Nucleotide sequence of the yeast plasmid."
Hartley J.L., Donelson J.E.
Nature 286:860-864(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A364A D5.
[2]"Sequence diversity of yeast 2 microns RAF gene and its co-evolution with STB and REP1."
Xiao W., Pelcher L.E., Rank G.H.
Gene 101:75-80(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4108 and ATCC 7754.
[3]"Antagonistic controls regulate copy number of the yeast 2 micron plasmid."
Murray J.A.H., Scarpa M., Rossi N., Cesareni G.
EMBO J. 6:4205-4212(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01347 Genomic DNA. Translation: AAB59342.1.
M57897 Genomic DNA. Translation: AAA34955.1.
M57898 Genomic DNA. Translation: AAA34956.1.
PIRJQ1027.
S22793.
S22872.
RefSeqNP_040490.1. NC_001398.1.

3D structure databases

ProteinModelPortalQ06891.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-518N.
IntActQ06891. 4 interactions.
STRING4932.R0030W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

CYGDR0030w.
SGDS000029674. RAF1.

Phylogenomic databases

OrthoDBEOG7Q8D0F.

Gene expression databases

GenevestigatorQ06891.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameRAF_YEAST
AccessionPrimary (citable) accession number: Q06891
Secondary accession number(s): Q06966, Q06977
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 1, 1996
Last modified: November 13, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD