ID   EGR3_HUMAN              Reviewed;         387 AA.
AC   Q06889; A8K8U9; B4DHJ5; E7EW38; Q2M3W2;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   24-JAN-2024, entry version 185.
DE   RecName: Full=Early growth response protein 3;
DE            Short=EGR-3;
DE   AltName: Full=Zinc finger protein pilot;
GN   Name=EGR3; Synonyms=PILOT;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Peripheral blood T-cell;
RX   PubMed=8443122; DOI=10.1093/intimm/5.1.63;
RA   Mages H.W., Stamminger T., Rilke O., Bravo R., Kroczek R.A.;
RT   "Expression of PILOT, a putative transcription factor, requires two signals
RT   and is cyclosporin A sensitive in T cells.";
RL   Int. Immunol. 5:63-70(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=1906159;
RA   Patwardhan S., Gashler A., Siegel M.G., Chang L.C., Joseph L.J.,
RA   Shows T.B., le Beau M.M., Sukhatme V.P.;
RT   "EGR3, a novel member of the Egr family of genes encoding immediate-early
RT   transcription factors.";
RL   Oncogene 6:917-928(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16421571; DOI=10.1038/nature04406;
RA   Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA   Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA   Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA   Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA   Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA   Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA   Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA   Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA   Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA   O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA   Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA   Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA   Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA   Platzer M., Shimizu N., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 8.";
RL   Nature 439:331-335(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Probable transcription factor involved in muscle spindle
CC       development.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q06889-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q06889-2; Sequence=VSP_045954;
CC   -!- DEVELOPMENTAL STAGE: In T-cells, expressed 20 minutes following
CC       activation.
CC   -!- SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; X63741; CAA45275.1; -; mRNA.
DR   EMBL; S40832; AAB19317.1; -; mRNA.
DR   EMBL; AK292464; BAF85153.1; -; mRNA.
DR   EMBL; AK295134; BAG58157.1; -; mRNA.
DR   EMBL; AK313604; BAG36369.1; -; mRNA.
DR   EMBL; AC105046; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC104765; AAI04766.1; -; mRNA.
DR   EMBL; BC112279; AAI12280.1; -; mRNA.
DR   CCDS; CCDS56528.1; -. [Q06889-2]
DR   CCDS; CCDS6033.1; -. [Q06889-1]
DR   PIR; S60519; S19885.
DR   RefSeq; NP_001186809.1; NM_001199880.1. [Q06889-2]
DR   RefSeq; NP_001186810.1; NM_001199881.1.
DR   RefSeq; NP_004421.2; NM_004430.2. [Q06889-1]
DR   RefSeq; XP_005273483.1; XM_005273426.3.
DR   AlphaFoldDB; Q06889; -.
DR   SMR; Q06889; -.
DR   IntAct; Q06889; 2.
DR   STRING; 9606.ENSP00000318057; -.
DR   GlyConnect; 2036; 1 N-Linked glycan (1 site).
DR   GlyCosmos; Q06889; 1 site, 1 glycan.
DR   GlyGen; Q06889; 1 site, 1 N-linked glycan (1 site).
DR   iPTMnet; Q06889; -.
DR   PhosphoSitePlus; Q06889; -.
DR   BioMuta; EGR3; -.
DR   DMDM; 730328; -.
DR   MassIVE; Q06889; -.
DR   PaxDb; 9606-ENSP00000318057; -.
DR   PeptideAtlas; Q06889; -.
DR   ProteomicsDB; 18765; -.
DR   ProteomicsDB; 58487; -. [Q06889-1]
DR   TopDownProteomics; Q06889-1; -. [Q06889-1]
DR   Antibodypedia; 22692; 265 antibodies from 28 providers.
DR   DNASU; 1960; -.
DR   Ensembl; ENST00000317216.3; ENSP00000318057.2; ENSG00000179388.9. [Q06889-1]
DR   Ensembl; ENST00000522910.1; ENSP00000430310.1; ENSG00000179388.9. [Q06889-2]
DR   GeneID; 1960; -.
DR   KEGG; hsa:1960; -.
DR   MANE-Select; ENST00000317216.3; ENSP00000318057.2; NM_004430.3; NP_004421.2.
DR   UCSC; uc003xcm.2; human. [Q06889-1]
DR   AGR; HGNC:3240; -.
DR   CTD; 1960; -.
DR   DisGeNET; 1960; -.
DR   GeneCards; EGR3; -.
DR   HGNC; HGNC:3240; EGR3.
DR   HPA; ENSG00000179388; Tissue enhanced (skin).
DR   MIM; 602419; gene.
DR   neXtProt; NX_Q06889; -.
DR   OpenTargets; ENSG00000179388; -.
DR   PharmGKB; PA27675; -.
DR   VEuPathDB; HostDB:ENSG00000179388; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000160355; -.
DR   HOGENOM; CLU_043235_0_0_1; -.
DR   InParanoid; Q06889; -.
DR   OMA; GEVEPMY; -.
DR   OrthoDB; 2912670at2759; -.
DR   PhylomeDB; Q06889; -.
DR   TreeFam; TF318980; -.
DR   PathwayCommons; Q06889; -.
DR   Reactome; R-HSA-9031628; NGF-stimulated transcription.
DR   SignaLink; Q06889; -.
DR   SIGNOR; Q06889; -.
DR   BioGRID-ORCS; 1960; 14 hits in 1173 CRISPR screens.
DR   ChiTaRS; EGR3; human.
DR   GeneWiki; EGR3; -.
DR   GenomeRNAi; 1960; -.
DR   Pharos; Q06889; Tbio.
DR   PRO; PR:Q06889; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; Q06889; Protein.
DR   Bgee; ENSG00000179388; Expressed in upper leg skin and 172 other cell types or tissues.
DR   ExpressionAtlas; Q06889; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0045202; C:synapse; IEA:GOC.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0002042; P:cell migration involved in sprouting angiogenesis; IDA:BHF-UCL.
DR   GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IMP:BHF-UCL.
DR   GO; GO:0035924; P:cellular response to vascular endothelial growth factor stimulus; IMP:BHF-UCL.
DR   GO; GO:0007623; P:circadian rhythm; TAS:ProtInc.
DR   GO; GO:0035767; P:endothelial cell chemotaxis; IMP:BHF-UCL.
DR   GO; GO:0007517; P:muscle organ development; TAS:ProtInc.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IMP:BHF-UCL.
DR   GO; GO:0007274; P:neuromuscular synaptic transmission; IEA:Ensembl.
DR   GO; GO:0007422; P:peripheral nervous system development; IEA:Ensembl.
DR   GO; GO:0001938; P:positive regulation of endothelial cell proliferation; IMP:BHF-UCL.
DR   GO; GO:0033089; P:positive regulation of T cell differentiation in thymus; IEA:Ensembl.
DR   GO; GO:0045586; P:regulation of gamma-delta T cell differentiation; IEA:Ensembl.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 3.
DR   InterPro; IPR021849; EGR_N.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR23235:SF78; EARLY GROWTH RESPONSE PROTEIN 3; 1.
DR   PANTHER; PTHR23235; KRUEPPEL-LIKE TRANSCRIPTION FACTOR; 1.
DR   Pfam; PF11928; DUF3446; 1.
DR   Pfam; PF00096; zf-C2H2; 3.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
DR   Genevisible; Q06889; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..387
FT                   /note="Early growth response protein 3"
FT                   /id="PRO_0000047125"
FT   ZN_FING         275..299
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         305..327
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         333..355
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          241..283
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          348..387
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        265..283
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..51
FT                   /note="MTGKLAEKLPVTMSSLLNQLPDNLYPEEIPSALNLFSGSSDSVVHYNQMAT
FT                   -> MEPCAAWSPRGGR (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_045954"
FT   CONFLICT        102
FT                   /note="I -> T (in Ref. 3; BAG58157)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        225
FT                   /note="I -> T (in Ref. 2; AAB19317)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   387 AA;  42613 MW;  77FAF7D5A278C68E CRC64;
     MTGKLAEKLP VTMSSLLNQL PDNLYPEEIP SALNLFSGSS DSVVHYNQMA TENVMDIGLT
     NEKPNPELSY SGSFQPAPGN KTVTYLGKFA FDSPSNWCQD NIISLMSAGI LGVPPASGAL
     STQTSTASMV QPPQGDVEAM YPALPPYSNC GDLYSEPVSF HDPQGNPGLA YSPQDYQSAK
     PALDSNLFPM IPDYNLYHHP NDMGSIPEHK PFQGMDPIRV NPPPITPLET IKAFKDKQIH
     PGFGSLPQPP LTLKPIRPRK YPNRPSKTPL HERPHACPAE GCDRRFSRSD ELTRHLRIHT
     GHKPFQCRIC MRSFSRSDHL TTHIRTHTGE KPFACEFCGR KFARSDERKR HAKIHLKQKE
     KKAEKGGAPS ASSAPPVSLA PVVTTCA
//