Q06889 (EGR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Early growth response protein 3
Short name=EGR-3

Alternative name(s):
Zinc finger protein pilot

Gene names

Name:	EGR3
Synonyms:	PILOT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor involved in muscle spindle development.
Subcellular location	Nucleus Probable.
Developmental stage	In T-cells, expressed 20 minutes following activation.
Sequence similarities	Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell migration involved in sprouting angiogenesis Inferred from direct assay PubMed 18059339. Source: BHF-UCL cellular response to fibroblast growth factor stimulus Inferred from mutant phenotype PubMed 18059339. Source: BHF-UCL cellular response to vascular endothelial growth factor stimulus Inferred from mutant phenotype PubMed 18059339. Source: BHF-UCL circadian rhythm Traceable author statement PubMed 9488654. Source: ProtInc endothelial cell chemotaxis Inferred from mutant phenotype PubMed 18059339. Source: BHF-UCL muscle organ development Traceable author statement PubMed 9731539. Source: ProtInc negative regulation of apoptotic process Inferred from mutant phenotype PubMed 18059339. Source: BHF-UCL neuromuscular synaptic transmission Inferred from electronic annotation. Source: Compara peripheral nervous system development Inferred from electronic annotation. Source: Compara positive regulation of endothelial cell proliferation Inferred from mutant phenotype PubMed 18059339. Source: BHF-UCL transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement Ref.2PubMed 9731539. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q06889-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q06889-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: MTGKLAEKLPVTMSSLLNQLPDNLYPEEIPSALNLFSGSSDSVVHYNQMAT → MEPCAAWSPRGGR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Early growth response protein 3		PRO_0000047125
Regions
	Zinc finger	275 – 299	25	C2H2-type 1
	Zinc finger	305 – 327	23	C2H2-type 2
	Zinc finger	333 – 355	23	C2H2-type 3
Natural variations
	Alternative sequence	1 – 51	51	MTGKL…NQMAT → MEPCAAWSPRGGR in isoform 2.		VSP_045954
Experimental info
	Sequence conflict	102	1	I → T in BAG58157. Ref.3
	Sequence conflict	225	1	I → T in AAB19317. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 77FAF7D5A278C68E	FASTA	387	42,613
	10 20 30 40 50 60 MTGKLAEKLP VTMSSLLNQL PDNLYPEEIP SALNLFSGSS DSVVHYNQMA TENVMDIGLT 70 80 90 100 110 120 NEKPNPELSY SGSFQPAPGN KTVTYLGKFA FDSPSNWCQD NIISLMSAGI LGVPPASGAL 130 140 150 160 170 180 STQTSTASMV QPPQGDVEAM YPALPPYSNC GDLYSEPVSF HDPQGNPGLA YSPQDYQSAK 190 200 210 220 230 240 PALDSNLFPM IPDYNLYHHP NDMGSIPEHK PFQGMDPIRV NPPPITPLET IKAFKDKQIH 250 260 270 280 290 300 PGFGSLPQPP LTLKPIRPRK YPNRPSKTPL HERPHACPAE GCDRRFSRSD ELTRHLRIHT 310 320 330 340 350 360 GHKPFQCRIC MRSFSRSDHL TTHIRTHTGE KPFACEFCGR KFARSDERKR HAKIHLKQKE 370 380 KKAEKGGAPS ASSAPPVSLA PVVTTCA
	Isoform 2 [UniParc]. Checksum: 32ED293BBFB500B7	FASTA	349	38,478
	10 20 30 40 50 60 MEPCAAWSPR GGRENVMDIG LTNEKPNPEL SYSGSFQPAP GNKTVTYLGK FAFDSPSNWC 70 80 90 100 110 120 QDNIISLMSA GILGVPPASG ALSTQTSTAS MVQPPQGDVE AMYPALPPYS NCGDLYSEPV 130 140 150 160 170 180 SFHDPQGNPG LAYSPQDYQS AKPALDSNLF PMIPDYNLYH HPNDMGSIPE HKPFQGMDPI 190 200 210 220 230 240 RVNPPPITPL ETIKAFKDKQ IHPGFGSLPQ PPLTLKPIRP RKYPNRPSKT PLHERPHACP 250 260 270 280 290 300 AEGCDRRFSR SDELTRHLRI HTGHKPFQCR ICMRSFSRSD HLTTHIRTHT GEKPFACEFC 310 320 330 340 GRKFARSDER KRHAKIHLKQ KEKKAEKGGA PSASSAPPVS LAPVVTTCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of PILOT, a putative transcription factor, requires two signals and is cyclosporin A sensitive in T cells." Mages H.W., Stamminger T., Rilke O., Bravo R., Kroczek R.A. Int. Immunol. 5:63-70(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Peripheral blood T-cell.
[2]	"EGR3, a novel member of the Egr family of genes encoding immediate-early transcription factors." Patwardhan S., Gashler A., Siegel M.G., Chang L.C., Joseph L.J., Shows T.B., le Beau M.M., Sukhatme V.P. Oncogene 6:917-928(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Testis.
[4]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63741 mRNA. Translation: CAA45275.1. S40832 mRNA. Translation: AAB19317.1. AK292464 mRNA. Translation: BAF85153.1. AK295134 mRNA. Translation: BAG58157.1. AK313604 mRNA. Translation: BAG36369.1. AC105046 Genomic DNA. No translation available. BC104765 mRNA. Translation: AAI04766.1. BC112279 mRNA. Translation: AAI12280.1.
IPI	IPI00300178. IPI00984563.
PIR	S19885. S60519.
RefSeq	NP_001186809.1. NM_001199880.1. NP_001186810.1. NM_001199881.1. NP_004421.2. NM_004430.2.
UniGene	Hs.534313.
3D structure databases
ProteinModelPortal	Q06889.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-234110.
STRING	9606.ENSP00000318057.
PTM databases
PhosphoSite	Q06889.
Polymorphism databases
DMDM	730328.
Proteomic databases
PaxDb	Q06889.
PRIDE	Q06889.
Protocols and materials databases
DNASU	1960.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317216; ENSP00000318057; ENSG00000179388. ENST00000522910; ENSP00000430310; ENSG00000179388.
GeneID	1960.
KEGG	hsa:1960.
UCSC	uc003xcm.1. human. uc011kzn.1. human.
Organism-specific databases
CTD	1960.
GeneCards	GC08M022545.
HGNC	HGNC:3240. EGR3.
HPA	CAB009373. HPA006206.
MIM	602419. gene.
neXtProt	NX_Q06889.
PharmGKB	PA27675.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000036856.
HOVERGEN	HBG003909.
InParanoid	Q06889.
KO	K12497.
OMA	QTMDPIR.
OrthoDB	EOG4TTGJ3.
PhylomeDB	Q06889.
Enzyme and pathway databases
Pathway_Interaction_DB	nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes.
SignaLink	Q06889.
Gene expression databases
ArrayExpress	Q06889.
Bgee	Q06889.
CleanEx	HS_EGR3.
Genevestigator	Q06889.
GermOnline	ENSG00000179388. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR021849. DUF3446. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF11928. DUF3446. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EGR3. human.
GenomeRNAi	1960.
NextBio	7951.
SOURCE	Search...

Entry information

Entry name

EGR3_HUMAN

Accession

Primary (citable) accession number: Q06889
Secondary accession number(s): A8K8U9 , B4DHJ5, E7EW38, Q2M3W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.