Q06886 (GUX1_PENJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exoglucanase 1
EC=3.2.1.91

Alternative name(s):
1,4-beta-cellobiohydrolase
Exocellobiohydrolase I
Exoglucanase I

Gene names

Name:

cbh1

Organism

Penicillium janthinellum (Penicillium vitale)

Taxonomic identifier

5079 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 7 (cellulase C) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 537	519	Exoglucanase 1		PRO_0000007923
Regions
	Domain	501 – 537	37	CBM1
	Region	19 – 453	435	Catalytic
	Region	454 – 477	24	Linker
Sites
	Active site	235	1	Nucleophile By similarity
	Active site	240	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	136	1	N-linked (GlcNAc...) Potential
	Glycosylation	414	1	N-linked (GlcNAc...) Potential
	Glycosylation	456	1	N-linked (GlcNAc...) Potential
	Disulfide bond	509 ↔ 526		By similarity
	Disulfide bond	520 ↔ 536		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06886 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A6B9C6EB73F17FE4	FASTA	537	56,845
	10 20 30 40 50 60 MKGSISYQIY KGALLLSALL NSVSAQQVGT LTAETHPALT WSKCTAGXCS QVSGSVVIDA 70 80 90 100 110 120 NWPXVHSTSG STNCYTGNTW DATLCPDDVT CAANCAVDGA RRQHLRVTTS GNSLRINFVT 130 140 150 160 170 180 TASQKNIGSR LYLLENDTTY QKFNLLNQEF TFDVDVSNLP CGLNGALYFV DMDADGGMAK 190 200 210 220 230 240 YPTNKAGAKY GTGYCDSQCP RDLKFINGQA NVDGWTPSKN DVNSGIGNHG SCCAEMDIWE 250 260 270 280 290 300 ANSISNAVTP HPCDTPSQTM CTGQRCGGTY STDRYGGTCD PDGCDFNPYR MGVTNFYGPG 310 320 330 340 350 360 ETIDTKSPFT VVTQFLTNDG TSTGTLSEIK RFYVQGGKVI GNPQSTIVGV SGNSITDSWC 370 380 390 400 410 420 NAQKSAFGDT NEFSKHGGMA GMGAGLADGM VLVMSLWDDH ASDMLWLDST YPTNATSTTP 430 440 450 460 470 480 GAKRGTCDIS RRPNTVESTY PNAYVIYSNI KTGPLNSTFT GGTTSSSSTT TTTSKSTSTS 490 500 510 520 530 SSSKTTTTVT TTTTSSGSSG TGARDWAQCG GNGWTGPTTC VSPYTCTKQN DWYSQCL

References

[1]	"Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum." Koch A., Weigel C.T.O., Schulz G. Gene 124:57-65(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C41.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59054 mRNA. Translation: CAA41780.1.
PIR	JU0150.
3D structure databases
ProteinModelPortal	Q06886.
SMR	Q06886. Positions 503-537.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH7. Glycoside Hydrolase Family 7.
mycoCLAP	CBH7A_PENJA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.70.100.10. 1 hit.
InterPro	IPR000254. Cellulose-bd_dom_fun. IPR008985. ConA-like_lec_gl_sf. IPR001722. Glyco_hydro_7. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF00840. Glyco_hydro_7. 1 hit. [Graphical view]
PRINTS	PR00734. GLHYDRLASE7.
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUX1_PENJA

Accession

Primary (citable) accession number: Q06886

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program