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Protein

Calcium-dependent protein kinase 1

Gene

CPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Phosphorylates the Ca2+-ATPase ACA2 resulting in the inhibition of its calcium activation.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei179 – 1791ATPPROSITE-ProRule annotation
Active sitei274 – 2741Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi156 – 1649ATPPROSITE-ProRule annotation
Calcium bindingi464 – 475121Add
BLAST
Calcium bindingi500 – 511122Add
BLAST
Calcium bindingi536 – 547123Add
BLAST
Calcium bindingi570 – 581124Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G04870-MONOMER.
BRENDAi2.7.11.1. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 1 (EC:2.7.11.1)
Short name:
AtCDPK 1
Short name:
CDPK 1
Alternative name(s):
Calcium-dependent protein kinase isoform AK1
Gene namesi
Name:CPK1
Synonyms:AK1
Ordered Locus Names:At5g04870
ORF Names:MUK11.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G04870.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • peroxisomal membrane Source: UniProtKB-SubCell
  • peroxisome Source: TAIR
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi436 – 4361F → A: Abolishes activation by calcium. 1 Publication
Mutagenesisi443 – 4442VI → AA: Abolishes activation by calcium. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 610609Calcium-dependent protein kinase 1PRO_0000085827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Lipidationi5 – 51S-palmitoyl cysteineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ06850.
PRIDEiQ06850.

PTM databases

iPTMnetiQ06850.

Expressioni

Gene expression databases

GenevisibleiQ06850. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi15645. 64 interactions.
IntActiQ06850. 1 interaction.
STRINGi3702.AT5G04870.1.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi434 – 44714Combined sources
Helixi450 – 46314Combined sources
Beta strandi469 – 4713Combined sources
Helixi473 – 4797Combined sources
Helixi480 – 4834Combined sources
Helixi489 – 49911Combined sources
Beta strandi505 – 5073Combined sources
Helixi509 – 5168Combined sources
Helixi520 – 5234Combined sources
Helixi526 – 53510Combined sources
Beta strandi540 – 5434Combined sources
Helixi545 – 5517Combined sources
Helixi565 – 5695Combined sources
Beta strandi574 – 5774Combined sources
Helixi579 – 5868Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AAOX-ray2.00A/B428-593[»]
ProteinModelPortaliQ06850.
SMRiQ06850. Positions 97-587.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06850.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 408259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini451 – 48636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini487 – 52236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini523 – 55836EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini559 – 59234EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni414 – 44431Autoinhibitory domainAdd
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (414-444) inactivates kinase activity under calcium-free conditions.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ06850.
KOiK13412.
OMAiSHESACK.
OrthoDBiEOG093605DK.
PhylomeDBiQ06850.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNTCVGPSR NGFLQSVSAA MWRPRDGDDS ASMSNGDIAS EAVSGELRSR
60 70 80 90 100
LSDEVQNKPP EQVTMPKPGT DVETKDREIR TESKPETLEE ISLESKPETK
110 120 130 140 150
QETKSETKPE SKPDPPAKPK KPKHMKRVSS AGLRTESVLQ RKTENFKEFY
160 170 180 190 200
SLGRKLGQGQ FGTTFLCVEK TTGKEFACKS IAKRKLLTDE DVEDVRREIQ
210 220 230 240 250
IMHHLAGHPN VISIKGAYED VVAVHLVMEC CAGGELFDRI IQRGHYTERK
260 270 280 290 300
AAELTRTIVG VVEACHSLGV MHRDLKPENF LFVSKHEDSL LKTIDFGLSM
310 320 330 340 350
FFKPDDVFTD VVGSPYYVAP EVLRKRYGPE ADVWSAGVIV YILLSGVPPF
360 370 380 390 400
WAETEQGIFE QVLHGDLDFS SDPWPSISES AKDLVRKMLV RDPKKRLTAH
410 420 430 440 450
QVLCHPWVQV DGVAPDKPLD SAVLSRMKQF SAMNKFKKMA LRVIAESLSE
460 470 480 490 500
EEIAGLKEMF NMIDADKSGQ ITFEELKAGL KRVGANLKES EILDLMQAAD
510 520 530 540 550
VDNSGTIDYK EFIAATLHLN KIEREDHLFA AFTYFDKDGS GYITPDELQQ
560 570 580 590 600
ACEEFGVEDV RIEELMRDVD QDNDGRIDYN EFVAMMQKGS ITGGPVKMGL
610
EKSFSIALKL
Length:610
Mass (Da):68,254
Last modified:February 1, 1995 - v1
Checksum:i41868DF12B0DF9FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14771 mRNA. Translation: AAA32761.1.
AB008271 Genomic DNA. Translation: BAB08991.1.
CP002688 Genomic DNA. Translation: AED90798.1.
AK118706 mRNA. Translation: BAC43300.1.
BT005932 mRNA. Translation: AAO64867.1.
PIRiA49082.
RefSeqiNP_196107.1. NM_120569.2.
UniGeneiAt.293.

Genome annotation databases

EnsemblPlantsiAT5G04870.1; AT5G04870.1; AT5G04870.
GeneIDi830366.
GrameneiAT5G04870.1; AT5G04870.1; AT5G04870.
KEGGiath:AT5G04870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14771 mRNA. Translation: AAA32761.1.
AB008271 Genomic DNA. Translation: BAB08991.1.
CP002688 Genomic DNA. Translation: AED90798.1.
AK118706 mRNA. Translation: BAC43300.1.
BT005932 mRNA. Translation: AAO64867.1.
PIRiA49082.
RefSeqiNP_196107.1. NM_120569.2.
UniGeneiAt.293.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AAOX-ray2.00A/B428-593[»]
ProteinModelPortaliQ06850.
SMRiQ06850. Positions 97-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15645. 64 interactions.
IntActiQ06850. 1 interaction.
STRINGi3702.AT5G04870.1.

PTM databases

iPTMnetiQ06850.

Proteomic databases

PaxDbiQ06850.
PRIDEiQ06850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04870.1; AT5G04870.1; AT5G04870.
GeneIDi830366.
GrameneiAT5G04870.1; AT5G04870.1; AT5G04870.
KEGGiath:AT5G04870.

Organism-specific databases

TAIRiAT5G04870.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ06850.
KOiK13412.
OMAiSHESACK.
OrthoDBiEOG093605DK.
PhylomeDBiQ06850.

Enzyme and pathway databases

BioCyciARA:AT5G04870-MONOMER.
BRENDAi2.7.11.1. 399.

Miscellaneous databases

EvolutionaryTraceiQ06850.
PROiQ06850.

Gene expression databases

GenevisibleiQ06850. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK1_ARATH
AccessioniPrimary (citable) accession number: Q06850
Secondary accession number(s): Q541W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.