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Protein

Calcium-dependent protein kinase 1

Gene

CPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Phosphorylates the Ca2+-ATPase ACA2 resulting in the inhibition of its calcium activation.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei179ATPPROSITE-ProRule annotation1
Active sitei274Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi156 – 164ATPPROSITE-ProRule annotation9
Calcium bindingi464 – 4751Combined sources2 PublicationsAdd BLAST12
Calcium bindingi500 – 5112Combined sources2 PublicationsAdd BLAST12
Calcium bindingi536 – 5473Combined sources2 PublicationsAdd BLAST12
Calcium bindingi570 – 5814Combined sources2 PublicationsAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 1 (EC:2.7.11.1)
Short name:
AtCDPK 1
Short name:
CDPK 1
Alternative name(s):
Calcium-dependent protein kinase isoform AK1
Gene namesi
Name:CPK1
Synonyms:AK1
Ordered Locus Names:At5g04870
ORF Names:MUK11.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G04870.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • peroxisomal membrane Source: UniProtKB-SubCell
  • peroxisome Source: TAIR
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi436F → A: Abolishes activation by calcium. 1 Publication1
Mutagenesisi443 – 444VI → AA: Abolishes activation by calcium. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000858272 – 610Calcium-dependent protein kinase 1Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Lipidationi5S-palmitoyl cysteineCurated1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ06850.
PRIDEiQ06850.

PTM databases

iPTMnetiQ06850.

Expressioni

Gene expression databases

GenevisibleiQ06850. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi15645. 64 interactors.
IntActiQ06850. 1 interactor.
STRINGi3702.AT5G04870.1.

Structurei

Secondary structure

1610
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi434 – 447Combined sources14
Helixi450 – 463Combined sources14
Beta strandi469 – 471Combined sources3
Helixi473 – 479Combined sources7
Helixi480 – 483Combined sources4
Helixi489 – 499Combined sources11
Beta strandi505 – 507Combined sources3
Helixi509 – 516Combined sources8
Helixi520 – 523Combined sources4
Helixi526 – 535Combined sources10
Beta strandi540 – 543Combined sources4
Helixi545 – 551Combined sources7
Helixi565 – 569Combined sources5
Beta strandi574 – 577Combined sources4
Helixi579 – 586Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AAOX-ray2.00A/B428-593[»]
ProteinModelPortaliQ06850.
SMRiQ06850.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06850.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 408Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini451 – 486EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini487 – 522EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini523 – 558EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini559 – 592EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni414 – 444Autoinhibitory domain1 PublicationAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (414-444) inactivates kinase activity under calcium-free conditions.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ06850.
KOiK13412.
OMAiSHESACK.
OrthoDBiEOG093605DK.
PhylomeDBiQ06850.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNTCVGPSR NGFLQSVSAA MWRPRDGDDS ASMSNGDIAS EAVSGELRSR
60 70 80 90 100
LSDEVQNKPP EQVTMPKPGT DVETKDREIR TESKPETLEE ISLESKPETK
110 120 130 140 150
QETKSETKPE SKPDPPAKPK KPKHMKRVSS AGLRTESVLQ RKTENFKEFY
160 170 180 190 200
SLGRKLGQGQ FGTTFLCVEK TTGKEFACKS IAKRKLLTDE DVEDVRREIQ
210 220 230 240 250
IMHHLAGHPN VISIKGAYED VVAVHLVMEC CAGGELFDRI IQRGHYTERK
260 270 280 290 300
AAELTRTIVG VVEACHSLGV MHRDLKPENF LFVSKHEDSL LKTIDFGLSM
310 320 330 340 350
FFKPDDVFTD VVGSPYYVAP EVLRKRYGPE ADVWSAGVIV YILLSGVPPF
360 370 380 390 400
WAETEQGIFE QVLHGDLDFS SDPWPSISES AKDLVRKMLV RDPKKRLTAH
410 420 430 440 450
QVLCHPWVQV DGVAPDKPLD SAVLSRMKQF SAMNKFKKMA LRVIAESLSE
460 470 480 490 500
EEIAGLKEMF NMIDADKSGQ ITFEELKAGL KRVGANLKES EILDLMQAAD
510 520 530 540 550
VDNSGTIDYK EFIAATLHLN KIEREDHLFA AFTYFDKDGS GYITPDELQQ
560 570 580 590 600
ACEEFGVEDV RIEELMRDVD QDNDGRIDYN EFVAMMQKGS ITGGPVKMGL
610
EKSFSIALKL
Length:610
Mass (Da):68,254
Last modified:February 1, 1995 - v1
Checksum:i41868DF12B0DF9FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14771 mRNA. Translation: AAA32761.1.
AB008271 Genomic DNA. Translation: BAB08991.1.
CP002688 Genomic DNA. Translation: AED90798.1.
AK118706 mRNA. Translation: BAC43300.1.
BT005932 mRNA. Translation: AAO64867.1.
PIRiA49082.
RefSeqiNP_196107.1. NM_120569.3.
UniGeneiAt.293.

Genome annotation databases

EnsemblPlantsiAT5G04870.1; AT5G04870.1; AT5G04870.
GeneIDi830366.
GrameneiAT5G04870.1; AT5G04870.1; AT5G04870.
KEGGiath:AT5G04870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14771 mRNA. Translation: AAA32761.1.
AB008271 Genomic DNA. Translation: BAB08991.1.
CP002688 Genomic DNA. Translation: AED90798.1.
AK118706 mRNA. Translation: BAC43300.1.
BT005932 mRNA. Translation: AAO64867.1.
PIRiA49082.
RefSeqiNP_196107.1. NM_120569.3.
UniGeneiAt.293.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AAOX-ray2.00A/B428-593[»]
ProteinModelPortaliQ06850.
SMRiQ06850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15645. 64 interactors.
IntActiQ06850. 1 interactor.
STRINGi3702.AT5G04870.1.

PTM databases

iPTMnetiQ06850.

Proteomic databases

PaxDbiQ06850.
PRIDEiQ06850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04870.1; AT5G04870.1; AT5G04870.
GeneIDi830366.
GrameneiAT5G04870.1; AT5G04870.1; AT5G04870.
KEGGiath:AT5G04870.

Organism-specific databases

TAIRiAT5G04870.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ06850.
KOiK13412.
OMAiSHESACK.
OrthoDBiEOG093605DK.
PhylomeDBiQ06850.

Enzyme and pathway databases

BRENDAi2.7.11.1. 399.

Miscellaneous databases

EvolutionaryTraceiQ06850.
PROiQ06850.

Gene expression databases

GenevisibleiQ06850. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK1_ARATH
AccessioniPrimary (citable) accession number: Q06850
Secondary accession number(s): Q541W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.