Q06826 (BMP4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bone morphogenetic protein 4
Short name=BMP-4

Alternative name(s):
Bone morphogenetic protein 2B
Short name=BMP-2B

Gene names

Name:	Bmp4
Synonyms:	Bmp-4, Dvr-4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction By similarity.
Subunit structure	Homodimer; disulfide-linked. Interacts with SOSTDC1, GREM2, RGMA, RGMB and RGMC. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Sequence similarities	Belongs to the TGF-beta family.

Ontologies

Keywords
Biological process	Chondrogenesis Differentiation Osteogenesis
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Cytokine Developmental protein Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway involved in heart induction Inferred from sequence or structural similarity. Source: UniProtKB BMP signaling pathway involved in nephric duct formation Inferred from sequence or structural similarity. Source: UniProtKB BMP signaling pathway involved in renal system segmentation Inferred from sequence or structural similarity. Source: UniProtKB BMP signaling pathway involved in ureter morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB SMAD protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB activation of MAPKK activity Inferred from sequence or structural similarity. Source: UniProtKB blood vessel endothelial cell proliferation involved in sprouting angiogenesis Inferred from sequence or structural similarity. Source: UniProtKB brain development Inferred from expression pattern PubMed 14643011. Source: RGD branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB cellular response to dexamethasone stimulus Inferred from direct assay PubMed 17537984. Source: UniProtKB chondrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB common-partner SMAD protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB deltoid tuberosity development Inferred from sequence or structural similarity. Source: UniProtKB endochondral ossification Inferred from sequence or structural similarity. Source: UniProtKB epithelial tube branching involved in lung morphogenesis Inferred from expression pattern PubMed 8769111. Source: RGD glomerular capillary formation Inferred from sequence or structural similarity. Source: UniProtKB growth Inferred from electronic annotation. Source: InterPro intermediate mesodermal cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB lymphoid progenitor cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB mesonephros development Inferred from sequence or structural similarity. Source: UniProtKB metanephric collecting duct development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of branch elongation involved in ureteric bud branching by BMP signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation involved in heart morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of glomerular mesangial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of glomerulus development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of immature T cell proliferation in thymus Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of mesenchymal cell proliferation involved in ureter development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of metanephric S-shaped body morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of metanephric comma-shaped body morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of mitosis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of oligodendrocyte differentiation Inferred from mutant phenotype PubMed 16388796. Source: RGD negative regulation of thymocyte apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB odontogenesis Inferred from sequence or structural similarity. Source: UniProtKB osteoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB ovarian follicle development Inferred from mutant phenotype PubMed 12801979. Source: RGD positive regulation of BMP signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA-dependent DNA replication Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of SMAD protein import into nucleus Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of bone mineralization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of branching involved in lung morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 17537984. Source: UniProtKB positive regulation of cardiac muscle fiber development Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of collagen biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of endothelial cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of endothelial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of kidney development Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of osteoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of pathway-restricted SMAD protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 7911662. Source: MGI protein localization to nucleus Inferred from sequence or structural similarity. Source: UniProtKB pulmonary artery endothelial tube morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB response to estradiol stimulus Inferred from expression pattern PubMed 16551644. Source: RGD response to mechanical stimulus Inferred from expression pattern PubMed 10404008PubMed 17272757. Source: RGD response to retinoic acid Inferred from expression pattern PubMed 8385738. Source: RGD retina development in camera-type eye Inferred from expression pattern PubMed 17069773. Source: RGD secondary heart field specification Inferred from sequence or structural similarity. Source: UniProtKB specification of ureteric bud anterior/posterior symmetry by BMP signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB steroid hormone mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB tendon cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB tongue morphogenesis Inferred from expression pattern PubMed 16828469. Source: RGD type B pancreatic cell development Inferred from sequence or structural similarity. Source: UniProtKB ureter epithelial cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB ureter smooth muscle cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay PubMed 15589212. Source: RGD extracellular space Inferred from direct assay PubMed 18758911. Source: RGD membrane-bounded vesicle Inferred from direct assay PubMed 18758911. Source: RGD proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	BMP receptor binding Inferred from sequence or structural similarity. Source: UniProtKB chemoattractant activity Inferred from sequence or structural similarity. Source: UniProtKB growth factor activity Inferred from direct assay PubMed 12475223. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 16245174. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 292	273			PRO_0000033862
	Chain	293 – 408	116	Bone morphogenetic protein 4		PRO_0000033863
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Disulfide bond	308 ↔ 373		By similarity
	Disulfide bond	337 ↔ 405		By similarity
	Disulfide bond	341 ↔ 407		By similarity
	Disulfide bond	372		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06826 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 61E92B4B8D5624F3	FASTA	408	46,541
	10 20 30 40 50 60 MIPGNRMLMV VLLCQVLLGG ATDASLIPET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT 70 80 90 100 110 120 LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEEQS QGTGLEYPER PASRANTVRS 130 140 150 160 170 180 FHHEEHLENI PGTSESSAFR FFFNLSSIPE NEVISSAELR LFREQVDQGP DWEQGFHRIN 190 200 210 220 230 240 IYEVMKPPAE MVPGHLITRL LDTRLVRHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT 250 260 270 280 290 300 HLHQTRTHQG QHVRISRSLP QGSGNWAQLR PLLVTFGHDG RGHTLTRRRA KRSPKHHPQR 310 320 330 340 350 360 SRKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL 370 380 390 400 VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM VVEGCGCR

References

[1]	"Cloning and sequence of bone morphogenetic protein 4 cDNA from fetal rat calvarial cell." Chen D., Feng J.Q., Feng M., Harris M.A., Mundy G.R., Harris S.E. Biochim. Biophys. Acta 1174:289-292(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22607 mRNA. Translation: CAA80329.1.
IPI	IPI00196867.
PIR	S38343.
UniGene	Rn.10318.
3D structure databases
ProteinModelPortal	Q06826.
SMR	Q06826. Positions 307-408.
ModBase	Search...
PTM databases
PhosphoSite	Q06826.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:2213. rat.
Organism-specific databases
RGD	2213. Bmp4.
Phylogenomic databases
eggNOG	NOG243555.
HOGENOM	HOG000249478.
HOVERGEN	HBG004860.
InParanoid	Q06826.
OrthoDB	EOG4JT05K.
Gene expression databases
ArrayExpress	Q06826.
Genevestigator	Q06826.
GermOnline	ENSRNOG00000009694. Rattus norvegicus.
Family and domain databases
InterPro	IPR001839. TGF-b_C. IPR001111. TGF-b_N. IPR015615. TGF-beta-rel. IPR017948. TGFb_CS. [Graphical view]
PANTHER	PTHR11848. PTHR11848. 1 hit.
Pfam	PF00019. TGF_beta. 1 hit. PF00688. TGFb_propeptide. 1 hit. [Graphical view]
SMART	SM00204. TGFB. 1 hit. [Graphical view]
PROSITE	PS00250. TGF_BETA_1. 1 hit. PS51362. TGF_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BMP4_RAT

Accession

Primary (citable) accession number: Q06826

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program