Reviewed, UniProtKB/Swiss-Prot Q06817 (SYG2_YEAST)

Names and origin

Protein names

Recommended name:
    Glycyl-tRNA synthetase 2
    EC=6.1.1.14
Alternative name(s):
    Glycine--tRNA ligase 2
      Short name=GlyRS 2
      Short name=GlyRS2

Gene names

Name:	GRS2
Ordered Locus Names:	YPR081C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Ref.3
Miscellaneous	Present with 2290 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Caution	GRS1, which appears to be a duplication of GRS2, is necessary and sufficient for both mitochondrial and cytoplasmic glycyl-tRNA synthetase activities, suggesting that GRS2 may not be essential.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Glycyl-tRNA synthetase 2		PRO_0000073006

Sequences

	Sequence		Length	Mass (Da)
	Q06817-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 91D36F04D1AF7F38	FASTA	618	71,018
	10 20 30 40 50 60 MPLMSNSERD KLESTLRRRF FYTPSFEIYG GVSGLFDLGP PGCQLQNNLI RLWREHFIME 70 80 90 100 110 120 ENMLQVDGPM LTPYDVLKTS GHVDKFTDWM CRNPKTGEYY RADHLIEQTL KKRLLDKDVN 130 140 150 160 170 180 PQDMKNMEKI LTTIDGFSGP ELNLVMQEYN INDPVTNDVL DALTSFNLMF ETKIGASGQL 190 200 210 220 230 240 KAFLRPETAQ GQFLNFNKLL EINQGKIPFA SASIGKSFRN EISPRSGLLR VREFLMAEIE 250 260 270 280 290 300 HFVDPLNKSH AKFNEVLNEE IPLLSRRLQE SGEVQLPVKM TIGEAVNSGM VENETLGYFM 310 320 330 340 350 360 ARVHQFLLNI GINKDKFRFR QHLKNEMAHY ATDCWDGEIL TSYGWIECVG CADRAAFDLT 370 380 390 400 410 420 VHSKKTGRSL TVKQKLDTPK ERTEWVVEVN KKFFGSKFKQ KAKLIESVLS KFSQDELIRR 430 440 450 460 470 480 HEELEKNGEF TCQVNGQIVK LDSSLVTIKM KTTLQHIREY IPNVIEPSFG LGRIIYCIFD 490 500 510 520 530 540 HCFQVRVDSE SRGFFSFPLQ IAPIKVFVTT ISNNDGFPAI LKRISQALRK REIYFKIDDS 550 560 570 580 590 600 NTSIGKKYAR NDELGTPFGI TIDFETIKDQ TVTLRERNSM RQVRGTITDV ISTIDKMLHN 610 PDESDWDKST FGLSPVKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"One of two genes encoding glycyl-tRNA synthetase in Saccharomyces cerevisiae provides mitochondrial and cytoplasmic functions." Turner R.J., Lovato M., Schimmel P. J. Biol. Chem. 275:27681-27688(2000) [PubMed: 10874035] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51033 Genomic DNA. Translation: AAB68130.1.
PIR	S69067.
RefSeq	NP_015406.1.
3D structure databases
HSSP	HSSP built from PDB template 1B76 based on UniProtKB P56206.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06817. 1 interaction.
Proteomic databases
PeptideAtlas	Q06817.
Genome annotation databases
Ensembl	YPR081C. Saccharomyces cerevisiae. [Contig view]
GeneID	856196.
GenomeReviews	Gene locus YPR081C in contig U00094_GR.
KEGG	sce:YPR081C.
NMPDR	fig|4932.3.peg.6543.
Organism-specific databases
CYGD	YPR081c.
SGD	S000006285. GRS2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06817.
OMA	Q06817. INENDSR.
Enzyme and pathway databases
BRENDA	6.1.1.14. 250.
Gene expression databases
GermOnline	YPR081C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002315. tRNA-synt_gly. IPR018160. tRNA-synth_gly_a2dimer_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR10745. tRNA-synt_gly. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01043. TRNASYNTHGLY.
TIGRFAMs	TIGR00389. glyS_dimeric. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981387.

Entry information

Entry name

SYG2_YEAST

Accession

Primary (citable) accession number: Q06817

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)