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Protein

Glycine--tRNA ligase 2

Gene

GRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity).By similarity1 Publication

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011SubstrateBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Binding sitei325 – 3251SubstrateBy similarity
Binding sitei325 – 3251Substrate; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi219 – 2213ATPBy similarity
Nucleotide bindingi229 – 2346ATPBy similarity
Nucleotide bindingi347 – 3482ATPBy similarity
Nucleotide bindingi470 – 4734ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • diadenosine tetraphosphate biosynthetic process Source: UniProtKB
  • glycyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34225-MONOMER.
BRENDAi6.1.1.14. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase 2 (EC:6.1.1.14)
Alternative name(s):
Diadenosine tetraphosphate synthetase
Short name:
AP-4-A synthetase
Glycyl-tRNA synthetase 2
Short name:
GlyRS 2
Short name:
GlyRS2
Gene namesi
Name:GRS2
Ordered Locus Names:YPR081C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR081C.
SGDiS000006285. GRS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Glycine--tRNA ligase 2PRO_0000073006Add
BLAST

Proteomic databases

MaxQBiQ06817.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36252. 39 interactions.
IntActiQ06817. 6 interactions.
MINTiMINT-2779696.

Structurei

3D structure databases

ProteinModelPortaliQ06817.
SMRiQ06817. Positions 8-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 2385Substrate bindingBy similarity
Regioni466 – 4705Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
InParanoidiQ06817.
KOiK01880.
OMAiMGWSELE.
OrthoDBiEOG092C1IOM.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR033731. GlyRS-like_core.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLMSNSERD KLESTLRRRF FYTPSFEIYG GVSGLFDLGP PGCQLQNNLI
60 70 80 90 100
RLWREHFIME ENMLQVDGPM LTPYDVLKTS GHVDKFTDWM CRNPKTGEYY
110 120 130 140 150
RADHLIEQTL KKRLLDKDVN PQDMKNMEKI LTTIDGFSGP ELNLVMQEYN
160 170 180 190 200
INDPVTNDVL DALTSFNLMF ETKIGASGQL KAFLRPETAQ GQFLNFNKLL
210 220 230 240 250
EINQGKIPFA SASIGKSFRN EISPRSGLLR VREFLMAEIE HFVDPLNKSH
260 270 280 290 300
AKFNEVLNEE IPLLSRRLQE SGEVQLPVKM TIGEAVNSGM VENETLGYFM
310 320 330 340 350
ARVHQFLLNI GINKDKFRFR QHLKNEMAHY ATDCWDGEIL TSYGWIECVG
360 370 380 390 400
CADRAAFDLT VHSKKTGRSL TVKQKLDTPK ERTEWVVEVN KKFFGSKFKQ
410 420 430 440 450
KAKLIESVLS KFSQDELIRR HEELEKNGEF TCQVNGQIVK LDSSLVTIKM
460 470 480 490 500
KTTLQHIREY IPNVIEPSFG LGRIIYCIFD HCFQVRVDSE SRGFFSFPLQ
510 520 530 540 550
IAPIKVFVTT ISNNDGFPAI LKRISQALRK REIYFKIDDS NTSIGKKYAR
560 570 580 590 600
NDELGTPFGI TIDFETIKDQ TVTLRERNSM RQVRGTITDV ISTIDKMLHN
610
PDESDWDKST FGLSPVKI
Length:618
Mass (Da):71,018
Last modified:November 1, 1996 - v1
Checksum:i91D36F04D1AF7F38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA. Translation: AAB68130.1.
BK006949 Genomic DNA. Translation: DAA11499.1.
PIRiS69067.
RefSeqiNP_015406.1. NM_001184178.1.

Genome annotation databases

EnsemblFungiiYPR081C; YPR081C; YPR081C.
GeneIDi856196.
KEGGisce:YPR081C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA. Translation: AAB68130.1.
BK006949 Genomic DNA. Translation: DAA11499.1.
PIRiS69067.
RefSeqiNP_015406.1. NM_001184178.1.

3D structure databases

ProteinModelPortaliQ06817.
SMRiQ06817. Positions 8-597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36252. 39 interactions.
IntActiQ06817. 6 interactions.
MINTiMINT-2779696.

Proteomic databases

MaxQBiQ06817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR081C; YPR081C; YPR081C.
GeneIDi856196.
KEGGisce:YPR081C.

Organism-specific databases

EuPathDBiFungiDB:YPR081C.
SGDiS000006285. GRS2.

Phylogenomic databases

GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
InParanoidiQ06817.
KOiK01880.
OMAiMGWSELE.
OrthoDBiEOG092C1IOM.

Enzyme and pathway databases

BioCyciYEAST:G3O-34225-MONOMER.
BRENDAi6.1.1.14. 984.

Miscellaneous databases

PROiQ06817.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR033731. GlyRS-like_core.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYG2_YEAST
AccessioniPrimary (citable) accession number: Q06817
Secondary accession number(s): D6W483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2290 molecules/cell in log phase SD medium.1 Publication

Caution

GRS1, which appears to be a duplication of GRS2, is necessary and sufficient for both mitochondrial and cytoplasmic glycyl-tRNA synthetase activities, suggesting that GRS2 may not be essential.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.