Reviewed, UniProtKB/Swiss-Prot Q06815 (MRL1_YEAST)

Names and origin

Protein names

Recommended name:
    Mannose 6-phosphate receptor-like protein 1

Gene names

Name:	MRL1
Ordered Locus Names:	YPR079W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sorting receptor involved in the transport of vacuolar enzymes from the Golgi complex to the vacuole. Involved in the delivery and maturation of PEP4 (vacuolar proteinase A) and PRB1 (vacuolar proteinase B). Ref.2
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the MRL1/IGF2R family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Domain	Signal Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vacuolar transport Ref.2 Inferred from genetic interaction. Source: SGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Ref.2 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct signal sequence binding Ref.2 Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FTH1	P38310	1	EBI-33784,EBI-20959
PEP1	P32319	1	EBI-33784,EBI-13123
PMC1	P38929	1	EBI-33784,EBI-3097
VPS35	P34110	1	EBI-33784,EBI-20415
VPS38	Q05919	1	EBI-33784,EBI-29972

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 381	358	Mannose 6-phosphate receptor-like protein 1		PRO_0000042715
Regions
	Topological domain	24 – 236	213	Lumenal Potential
	Transmembrane	237 – 257	21	Potential
	Topological domain	258 – 381	124	Cytoplasmic Potential
Amino acid modifications
	Modified residue	334	1	Phosphoserine Ref.7
	Modified residue	335	1	Phosphoserine Ref.7 Ref.4 Ref.5 Ref.6
	Modified residue	337	1	Phosphoserine Ref.7 Ref.6
	Modified residue	339	1	Phosphoserine Ref.7 Ref.4 Ref.5
	Modified residue	340	1	Phosphoserine Ref.7
	Modified residue	342	1	Phosphoserine Ref.5
	Modified residue	371	1	Phosphoserine Ref.7
	Modified residue	380	1	Phosphoserine Ref.7
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	136	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06815-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4E89311978E85A4F	FASTA	381	42,706
	10 20 30 40 50 60 MLKRSSLIYL SCVLIITIPI LLHVYNGPGL SHEANEHRAS HKQKRTLANP DKPKSENDED 70 80 90 100 110 120 LFCAVTNPVT GSYIDLSQLS STPNKLREGQ KQISGNNKHE SSKTKWSVRG WGYDTNFTLG 130 140 150 160 170 180 ICSSPVGEAE SQQLSNLTGA FYVDQLNENN LVSIGDFSTR PALVGGSTAK KLTLKYENGS 190 200 210 220 230 240 MCPNGKDKKA TLLNFVCDKE IQSKAQISYI GNLHNCSYFF EVRSIHACPT SNKKNEVNVL 250 260 270 280 290 300 GIFIGIFAIF FLVEFAGRRW IYAKLNRHLK NDDELHDISP SLNEQPHWDL IEDGSRWSKF 310 320 330 340 350 360 FNGIIKTTRR FTKSLMRSLV RGRNSRQGGI RLRSSPSASS SSLANREFFR DMEAQNEIID 370 380 SLDINSHTTE SDHPTLADNS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A yeast homolog of the mammalian mannose 6-phosphate receptors contributes to the sorting of vacuolar hydrolases." Whyte J.R., Munro S. Curr. Biol. 11:1074-1078(2001) [PubMed: 11470415] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-339, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335; SER-339 AND SER-342, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-337, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-334; SER-335; SER-337; SER-339; SER-340; SER-371 AND SER-380, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51033 Genomic DNA. Translation: AAB68128.1.
PIR	S69065.
RefSeq	NP_015404.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2873N.
IntAct	Q06815. 7 interactions.
STRING	Q06815.
Proteomic databases
PeptideAtlas	Q06815.
Genome annotation databases
Ensembl	YPR079W; YPR079W; YPR079W; Saccharomyces cerevisiae. [Genome view]
GeneID	856194.
KEGG	sce:YPR079W.
NMPDR	fig|4932.3.peg.6541.
Organism-specific databases
CYGD	YPR079w.
SGD	S000006283. MRL1.
Phylogenomic databases
HOGENOM	Q06815.
OMA	KYENGSM
OrthoDB	EOG9B5QP5
Gene expression databases
ArrayExpress	Q06815.
Genevestigator	Q06815.
GermOnline	YPR079W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009011. Man6P_isomerase_rcpt_bd. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981381.

Entry information

Entry name

MRL1_YEAST

Accession

Primary (citable) accession number: Q06815

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)