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Protein

Calreticulin

Gene
N/A
Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071CarbohydrateBy similarity
Binding sitei109 – 1091CarbohydrateBy similarity
Binding sitei126 – 1261CarbohydrateBy similarity
Binding sitei133 – 1331CarbohydrateBy similarity
Binding sitei315 – 3151CarbohydrateBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  1. protein folding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Alternative name(s):
Protein SM4
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 393377CalreticulinPRO_0000004183Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi103 ↔ 135By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ06814.

Structurei

3D structure databases

ProteinModelPortaliQ06814.
SMRiQ06814. Positions 204-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati189 – 200121-1Add
BLAST
Repeati208 – 219121-2Add
BLAST
Repeati225 – 236121-3Add
BLAST
Repeati242 – 253121-4Add
BLAST
Repeati257 – 267112-1Add
BLAST
Repeati271 – 281112-2Add
BLAST
Repeati285 – 295112-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 253654 X 12 AA approximate repeatsAdd
BLAST
Regioni257 – 295393 X 11 AA approximate repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi390 – 3934Prevents secretion from ER

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi350 – 38940Asp/Glu/Lys-richAdd
BLAST

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000192435.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSILLTLLL SKYALGHEVW FSETFPNESI ENWVQSTYNA EKQGEFKVEA
60 70 80 90 100
GKSPVDPIED LGLKTTQDAR FYGIARKISE PFSNRGKTMV LQFTVKFDKT
110 120 130 140 150
VSCGGAYIKL LGSDIDPKKF HGESPYKIMF GPDICGMATK KVHVIFNYKG
160 170 180 190 200
KNHLIKKEIP CKDDLKTHLY TLIVNPNNKY EVLVDNAKVE EGSLEDDWDM
210 220 230 240 250
LPPKKIDDPN DKKPDDWVDE QFIDDPDDKK PDNWDQPKTI PDMDAKKPDD
260 270 280 290 300
WDDAMDGEWE RPQKDNPEYK GEWTPRRIDN PKYKGEWKPV QIDNPEYKHD
310 320 330 340 350
PELYVLNDIG YVGFDLWQVD SGSIFDNILI TDSPDFAKEE GERLWRKRYD
360 370 380 390
AEVAKEQSSA KDDKEEAEET KERKELPYDA KASDEPSGDH DEL
Length:393
Mass (Da):45,398
Last modified:February 1, 1996 - v2
Checksum:i45F59857C21940D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 902MV → IL in AAA19024 (PubMed:8139623).Curated
Sequence conflicti188 – 20720Missing in AAA19024 (PubMed:8139623).CuratedAdd
BLAST
Sequence conflicti378 – 3781Y → D in AAA19024 (PubMed:8139623).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93097 mRNA. Translation: AAA29854.1.
L24159 Genomic DNA. Translation: AAA19024.1.
PIRiA48573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93097 mRNA. Translation: AAA29854.1.
L24159 Genomic DNA. Translation: AAA19024.1.
PIRiA48573.

3D structure databases

ProteinModelPortaliQ06814.
SMRiQ06814. Positions 204-303.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ06814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000192435.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the gene encoding a Schistosoma mansoni antigen homologous to human Ro/SS-A autoantigen."
    Khalife J., Trottein F., Schacht A.-M., Godin C., Pierce R.J., Capron A.
    Mol. Biochem. Parasitol. 57:193-202(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Puerto Rican.
  2. "Cloning and sequencing of the gene encoding Schistosoma mansoni calreticulin."
    Khalife J., Pierce R.J., Godin C., Capron A.
    Mol. Biochem. Parasitol. 62:313-315(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Puerto Rican.

Entry informationi

Entry nameiCALR_SCHMA
AccessioniPrimary (citable) accession number: Q06814
Secondary accession number(s): Q26562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.