Q06814 (CALR_SCHMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calreticulin Alternative name(s): Protein SM4
Organism	Schistosoma mansoni (Blood fluke)
Taxonomic identifier	6183 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 393	377	Calreticulin		PRO_0000004183
Regions
	Repeat	189 – 200	12	1-1
	Repeat	208 – 219	12	1-2
	Repeat	225 – 236	12	1-3
	Repeat	242 – 253	12	1-4
	Repeat	257 – 267	11	2-1
	Repeat	271 – 281	11	2-2
	Repeat	285 – 295	11	2-3
	Region	189 – 253	65	4 X 12 AA approximate repeats
	Region	257 – 295	39	3 X 11 AA approximate repeats
	Motif	390 – 393	4	Prevents secretion from ER
	Compositional bias	350 – 389	40	Asp/Glu/Lys-rich
Sites
	Binding site	107	1	Carbohydrate By similarity
	Binding site	109	1	Carbohydrate By similarity
	Binding site	126	1	Carbohydrate By similarity
	Binding site	133	1	Carbohydrate By similarity
	Binding site	315	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 135		By similarity
Experimental info
	Sequence conflict	89 – 90	2	MV → IL in AAA19024. Ref.2
	Sequence conflict	188 – 207	20	Missing in AAA19024. Ref.2
	Sequence conflict	378	1	Y → D in AAA19024. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q06814 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 45F59857C21940D2	FASTA	393	45,398
	10 20 30 40 50 60 MLSILLTLLL SKYALGHEVW FSETFPNESI ENWVQSTYNA EKQGEFKVEA GKSPVDPIED 70 80 90 100 110 120 LGLKTTQDAR FYGIARKISE PFSNRGKTMV LQFTVKFDKT VSCGGAYIKL LGSDIDPKKF 130 140 150 160 170 180 HGESPYKIMF GPDICGMATK KVHVIFNYKG KNHLIKKEIP CKDDLKTHLY TLIVNPNNKY 190 200 210 220 230 240 EVLVDNAKVE EGSLEDDWDM LPPKKIDDPN DKKPDDWVDE QFIDDPDDKK PDNWDQPKTI 250 260 270 280 290 300 PDMDAKKPDD WDDAMDGEWE RPQKDNPEYK GEWTPRRIDN PKYKGEWKPV QIDNPEYKHD 310 320 330 340 350 360 PELYVLNDIG YVGFDLWQVD SGSIFDNILI TDSPDFAKEE GERLWRKRYD AEVAKEQSSA 370 380 390 KDDKEEAEET KERKELPYDA KASDEPSGDH DEL

References

[1]	"Cloning of the gene encoding a Schistosoma mansoni antigen homologous to human Ro/SS-A autoantigen." Khalife J., Trottein F., Schacht A.-M., Godin C., Pierce R.J., Capron A. Mol. Biochem. Parasitol. 57:193-202(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Puerto Rican.
[2]	"Cloning and sequencing of the gene encoding Schistosoma mansoni calreticulin." Khalife J., Pierce R.J., Godin C., Capron A. Mol. Biochem. Parasitol. 62:313-315(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Puerto Rican.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M93097 mRNA. Translation: AAA29854.1. L24159 Genomic DNA. Translation: AAA19024.1.
PIR	A48573.
3D structure databases
ProteinModelPortal	Q06814.
SMR	Q06814. Positions 204-303.
ModBase	Search...
Proteomic databases
PRIDE	Q06814.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HOG000192435.
Family and domain databases
Gene3D	2.60.120.200. 2 hits.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 1 hit. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_SCHMA

Accession

Primary (citable) accession number: Q06814
Secondary accession number(s): Q26562

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)