Q06811 (ABA1_ASCSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyprotein ABA-1

Alternative name(s):
Body fluid allergen 1
Nematode polyprotein allergen ABA-1
Short name=NPA ABA-1
Allergen=Asc s 1

Cleaved into the following 6 chains:

1. ABA-1B1 repeat unit
2. ABA-1A4 repeat unit
3. ABA-1A3 repeat unit
4. ABA-1A2 repeat unit
5. ABA-1A1 repeat unit
6. C-terminal extension peptide

Gene names

Name:

ABA-1

Organism

Ascaris suum (Pig roundworm) (Ascaris lumbricoides)

Taxonomic identifier

6253 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Ascaridida › Ascaridoidea › Ascarididae › Ascaris

Protein attributes

Sequence length	1365 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has high binding affinity for fatty acids and retinoids.
Tissue specificity	Pseudocoelomic fluid.
Post-translational modification	Nematode polyprotein allergens (NPAs) are synthesized as large polypeptides that are subsequently proteolytically cleaved to active polypeptide units.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the NPA family.

Ontologies

Keywords
Disease	Allergen
Domain	Repeat
Ligand	Lipid-binding Retinol-binding
PTM	Cleavage on pair of basic residues
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	retinol binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 1365

›1365

Polyprotein ABA-1

PRO_0000287126

Peptide

‹1 – 133

›133

ABA-1B1 repeat unit Probable

PRO_0000042221

Peptide

134 – 266

133

ABA-1A4 repeat unit Probable

PRO_0000042222

Peptide

267 – 399

133

ABA-1A3 repeat unit Probable

PRO_0000042223

Peptide

400 – 533

134

ABA-1A2 repeat unit Probable

PRO_0000042224

Peptide

534 – 666

133

ABA-1A1 repeat unit Probable

PRO_0000042225

Peptide

667 – 799

133

ABA-1A1 repeat unit Probable

PRO_0000042226

Peptide

800 – 932

133

ABA-1A1 repeat unit Probable

PRO_0000042227

Peptide

933 – 1065

133

ABA-1A1 repeat unit Probable

PRO_0000042228

Peptide

1066 – 1198

133

ABA-1A1 repeat unit Probable

PRO_0000020606

Peptide

1199 – 1331

133

ABA-1A1 repeat unit Probable

PRO_0000020607

Peptide

1332 – 1365

34

C-terminal extension peptide Potential

PRO_0000020608

Experimental info

Non-terminal residue

1

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1365

Helix Strand Turn

Details...

Beta strand

534 – 536

3

Helix

539 – 541

3

Turn

542 – 548

7

Helix

551 – 562

12

Helix

567 – 579

13

Helix

583 – 604

22

Helix

606 – 619

14

Helix

623 – 633

11

Helix

640 – 648

9

Helix

650 – 656

7

Sequences

	Sequence		Length	Mass (Da)
	Q06811 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: F7F43B78606863DD	FASTA	1,365	156,880
	10 20 30 40 50 60 TMEHYLKTYL SWLTEEQKEK LKEMKEAGKT KAEIQHEVMH YYDQLHGEEK QQATEKLKVG 70 80 90 100 110 120 CKMLLKGIIG EEKVVELRNM KEAGADIQEL QQKVEKMLSE VTDEKQKEKV HEYGPACKKI 130 140 150 160 170 180 FGATTLQHHR RRRHHFTLES SLDTHLKWLS QEQKDELLKM KKDGKTKKEL EAKILHYYDE 190 200 210 220 230 240 LEGDAKKEAT EHLKGGCGEI LKHVVGEEKA AELKNLKDSG ASKEELKAKV EEALHAVTDE 250 260 270 280 290 300 EKKQYIADFG PACKKIYGVH TSRRRRHHFT LESSLDTHLK WLSQEQKDEL LKMKKDGKAK 310 320 330 340 350 360 KELEAKILHY YDELEGDAKK EATEHLKGGC AEILKHVVGE EKAAELKNLK DSGASKEELK 370 380 390 400 410 420 AKVEEALHAV TDEEKKQYIA DFGPACKKIY GVHTSRRRRH HFTLESSLDT HLKWLSQEQK 430 440 450 460 470 480 DELLKMKKDG KTKKDLQAKI LHYYDELEGD AKKEATEHLK DGCREILKHV VGEEKEAELK 490 500 510 520 530 540 KLKDSGASKE EVKAKVEEAL HAVTDEEKKQ YIADFGPACK KIFGAAHTSR RRRHHFTLES 550 560 570 580 590 600 SLDTHLKWLS QEQKDELLKM KKDGKAKKEL EAKILHYYDE LEGDAKKEAT EHLKGGCREI 610 620 630 640 650 660 LKHVVGEEKA AELKNLKDSG ASKEELKAKV EEALHAVTDE EKKQYIADFG PACKKIYGVH 670 680 690 700 710 720 TSRRRRHHFT LESSLDTHLK WLSQEQKDEL LKMKKDGKAK KELEAKILHY YDELEGDAKK 730 740 750 760 770 780 EATEHLKGGC REILKHVVGE EKAAELKNLK DSGASKEELK AKVEEALHAV TDEEKKQYIA 790 800 810 820 830 840 DFGPACKKIY GVHTSRRRRH HFTLESSLDT HLKWLSQEQK DELLKMKKDG KAKKELEAKI 850 860 870 880 890 900 LHYYDELEGD AKKEATEHLK GGCREILKHV VGEEKAAELK NLKDSGASKE ELKAKVEEAL 910 920 930 940 950 960 HAVTDEEKKQ YIADFGPACK KIYGVHTSRR RRHHFTLESS LDTHLKWLSQ EQKDELLKMK 970 980 990 1000 1010 1020 KDGKAKKELE AKILHYYDEL EGDAKKEATE HLKGGCREIL KHVVGEEKAA ELKNLKDSGA 1030 1040 1050 1060 1070 1080 SKEELKAKVE EALHAVTDEE KKQYIADFGP ACKKIYGVHT SRRRRHHFTL ESSLDTHLKW 1090 1100 1110 1120 1130 1140 LSQEQKDELL KMKKDGKAKK ELEAKILHYY DELEGDAKKE ATEHLKGGCR EILKHVVGEE 1150 1160 1170 1180 1190 1200 KAAELKNLKD SGASKEELKA KVEEALHAVT DEEKKQYIAD FGPACKKIYG VHTSRRRRHH 1210 1220 1230 1240 1250 1260 FTLESSLDTH LKWLSQEQKD ELLKMKKDGK AKKELEAKIL HYYDELEGDA KKEATEHLKG 1270 1280 1290 1300 1310 1320 GCREILKHVV GEEKAAELKN LKDSGASKEE LKAKVEEALH AVTDEEKKQY IADFGPACKK 1330 1340 1350 1360 IYGVHTSRRR RYHAEDGTDD IDGLAQSRQR RSGFFEKLID VFAFF

References

[1]	"Sequence-divergent units of the ABA-1 polyprotein array of the nematode Ascaris suum have similar fatty-acid- and retinol-binding properties but different binding-site environments." Moore J., McDermott L., Price N.C., Kelly S.M., Cooper A., Kennedy M.W. Biochem. J. 340:337-343(1999) [PubMed: 10229690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-1095.
[2]	"A cDNA encoding repeating units of the ABA-1 allergen of Ascaris." Spence H.J., Moore J., Brass A., Kennedy M.W. Mol. Biochem. Parasitol. 57:339-343(1993) [PubMed: 8433722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 971-1365. Tissue: Larva.
[3]	"The ABA-1 allergen of the nematode Ascaris suum: epitope stability, mass spectrometry, and N-terminal sequence comparison with its homologue in Toxocara canis." Christie J.F., Dunbar B., Kennedy M.W. Clin. Exp. Immunol. 92:125-132(1993) [PubMed: 7682160] [Abstract] Cited for: PROTEIN SEQUENCE OF 1066-1133.
[4]	"N-terminal amino acid sequence identity between a major allergen of Ascaris lumbricoides and Ascaris suum, and MHC-restricted IgE responses to it." Christie J.F., Dunbar B., Davidson I., Kennedy M.W. Immunology 69:596-602(1990) [PubMed: 2335378] [Abstract] Cited for: PROTEIN SEQUENCE OF 1066-1106.
[5]	"Identification of the major Ascaris allergen and its purification to homogeneity by high-performance liquid chromatography." McGibbon A.M., Christie J.F., Kennedy M.W., Lee T.D. Mol. Biochem. Parasitol. 39:163-171(1990) [PubMed: 1690856] [Abstract] Cited for: PROTEIN SEQUENCE OF 1066-1075.
[6]	"The ABA-1 allergen of the parasitic nematode Ascaris suum: fatty acid and retinoid binding function and structural characterization." Kennedy M.W., Brasss A., McCruden A.B., Price N.C., Kelly S.M., Cooper A. Biochemistry 34:6700-6710(1995) [PubMed: 7756301] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF051702 mRNA. Translation: AAC06015.1.
L03211 mRNA. Translation: AAB41117.1.

PIR

A48576.
T31423.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XV9	NMR	-	A	534-662	[»]

ModBase

Search...

Protein family/group databases

Allergome

3541. Asc s 1.0101.
61. Asc s 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

ABA1_ASCSU

Accession

Primary (citable) accession number: Q06811
Secondary accession number(s): O61566, Q9TWU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 11, 2005
Last modified:	December 14, 2011
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)