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Protein

Tyrosine-protein kinase receptor Tie-1

Gene

Tie1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei866ATPPROSITE-ProRule annotation1
Active sitei975Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi841 – 849ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • blood vessel development Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of angiogenesis Source: MGI
  • negative regulation of cell migration Source: MGI
  • plasma membrane fusion Source: MGI
  • response to retinoic acid Source: BHF-UCL
  • vasculogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Angiogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase receptor Tie-1 (EC:2.7.10.1)
Gene namesi
Name:Tie1
Synonyms:Tie, Tie-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:99906. Tie1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 755ExtracellularSequence analysisAdd BLAST733
Transmembranei756 – 780HelicalSequence analysisAdd BLAST25
Topological domaini781 – 1134CytoplasmicSequence analysisAdd BLAST354

GO - Cellular componenti

  • integral component of membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2034800.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002447223 – 1134Tyrosine-protein kinase receptor Tie-1Add BLAST1112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi226 ↔ 235By similarity
Disulfide bondi229 ↔ 242By similarity
Disulfide bondi244 ↔ 253By similarity
Disulfide bondi266 ↔ 276By similarity
Disulfide bondi270 ↔ 289By similarity
Disulfide bondi291 ↔ 300By similarity
Disulfide bondi313 ↔ 325By similarity
Disulfide bondi319 ↔ 331By similarity
Disulfide bondi333 ↔ 342By similarity
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Glycosylationi592N-linked (GlcNAc...)Sequence analysis1
Glycosylationi705N-linked (GlcNAc...)Sequence analysis1
Modified residuei1003Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ06806.
PaxDbiQ06806.
PeptideAtlasiQ06806.
PRIDEiQ06806.

PTM databases

iPTMnetiQ06806.
PhosphoSitePlusiQ06806.

Expressioni

Tissue specificityi

Specifically expressed in developing vascular endothelial cells. Abundantly expressed in lung and heart, moderately in brain, liver and kidney, and weakly in thymus, spleen and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000033191.
CleanExiMM_TIE1.
ExpressionAtlasiQ06806. baseline and differential.
GenevisibleiQ06806. MM.

Interactioni

Subunit structurei

Heterodimer with TEK/TIE2.By similarity

Protein-protein interaction databases

IntActiQ06806. 1 interactor.
MINTiMINT-8020582.
STRINGi10090.ENSMUSP00000037129.

Chemistry databases

BindingDBiQ06806.

Structurei

3D structure databases

ProteinModelPortaliQ06806.
SMRiQ06806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 123Ig-like C2-type 1Add BLAST81
Domaini212 – 254EGF-like 1PROSITE-ProRule annotationAdd BLAST43
Domaini256 – 301EGF-like 2PROSITE-ProRule annotationAdd BLAST46
Domaini303 – 343EGF-like 3PROSITE-ProRule annotationAdd BLAST41
Domaini349 – 440Ig-like C2-type 2Add BLAST92
Domaini444 – 543Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini546 – 638Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST93
Domaini642 – 736Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST95
Domaini835 – 1114Protein kinasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.PROSITE-ProRule annotation
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOGENOMiHOG000049232.
HOVERGENiHBG007316.
InParanoidiQ06806.
KOiK05120.
OMAiHNSPGAH.
OrthoDBiEOG091G00RL.
TreeFamiTF317568.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF00047. ig. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00181. EGF. 3 hits.
SM00060. FN3. 3 hits.
SM00409. IG. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVWWGSSLLL PTLFLASHVG ASVDLTLLAN LRITDPQRFF LTCVSGEAGA
60 70 80 90 100
GRSSDPPLLL EKDDRIVRTF PPGQPLYLAR NGSHQVTLRG FSKPSDLVGV
110 120 130 140 150
FSCVGGAGAR RTRVLYVHNS PGAHLFPDKV THTVNKGDTA VLSAHVHKEK
160 170 180 190 200
QTDVIWKNNG SYFNTLDWQE ADDGRFQLQL QNVQPPSSGI YSATYLEASP
210 220 230 240 250
LGSAFFRLIV RGCGAGRWGP GCVKDCPGCL HGGVCHDHDG ECVCPPGFTG
260 270 280 290 300
TRCEQACREG RFGQSCQEQC PGTAGCRGLT FCLPDPYGCS CGSGWRGSQC
310 320 330 340 350
QEACAPGHFG ADCRLQCQCQ NGGTCDRFSG CVCPSGWHGV HCEKSDRIPQ
360 370 380 390 400
ILSMATEVEF NIGTMPRINC AAAGNPFPVR GSMKLRKPDG TMLLSTKVIV
410 420 430 440 450
EPDRTTAEFE VPSLTLGDSG FWECRVSTSG GQDSRRFKVN VKVPPVPLTA
460 470 480 490 500
PRLLAKQSRQ LVVSPLVSFS GDGPISSVRL HYRPQDSTIA WSAIVVDPSE
510 520 530 540 550
NVTLMNLKPK TGYNVRVQLS RPGEGGEGGW GPSALMTTDC PEPLLQPWLE
560 570 580 590 600
SWHVEGPDRL RVSWSLPSVP LSGDGFLLRL WDGARGQERR ENISFPQART
610 620 630 640 650
ALLTGLTPGT HYQLDVRLYH CTLLGPASPP AHVHLPPSGP PAPRHLHAQA
660 670 680 690 700
LSDSEIQLMW QHPEAPSGPI SKYIVEIQVA GGSGDPQWMD VDRPEETSII
710 720 730 740 750
VRGLNASTRY LFRVRASVQG LGDWSNTVEE ATLGNGLQSE GPVRESRAAE
760 770 780 790 800
EGLDQQLVLA VVGSVSATCL TILAALLALV CIRRSCLHRR RTFTYQSGSG
810 820 830 840 850
EETILQFSSG TLTLTRRPKP QPEPLSYPVL EWEDITFEDL IGEGNFGQVI
860 870 880 890 900
RAMIKKDGLK MNAAIKMLKE YASENDHRDF AGELEVLCKL GHHPNIINLL
910 920 930 940 950
GACENRGYLY IAIEYAPYGN LLDFLRKSRV LETDPAFARE HGTASTLSSR
960 970 980 990 1000
QLLRFASDAA NGMQYLSEKQ FIHRDLAARN VLVGENLASK IADFGLSRGE
1010 1020 1030 1040 1050
EVYVKKTMGR LPVRWMAIES LNYSVYTTKS DVWSFGVLLW EIVSLGGTPY
1060 1070 1080 1090 1100
CGMTCAELYE KLPQGYRMEQ PRNCDDEVYE LMRQCWRDRP YERPPFAQIA
1110 1120 1130
LQLGRMLEAR KAYVNMSLFE NFTYAGIDAT AEEA
Length:1,134
Mass (Da):124,583
Last modified:July 27, 2011 - v3
Checksum:i9669098A843B36F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti307G → D in CAA50556 (PubMed:8415706).Curated1
Sequence conflicti307G → D in CAA56739 (PubMed:8415706).Curated1
Sequence conflicti307G → D in CAA52148 (PubMed:8395828).Curated1
Sequence conflicti307G → D in AAH46452 (PubMed:15489334).Curated1
Sequence conflicti599R → L in CAA50556 (PubMed:8415706).Curated1
Sequence conflicti741G → D in CAA50556 (PubMed:8415706).Curated1
Sequence conflicti741G → D in CAA56739 (PubMed:8415706).Curated1
Sequence conflicti741G → D in CAA52148 (PubMed:8395828).Curated1
Sequence conflicti741G → D in AAH46452 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71425 mRNA. Translation: CAA50556.1.
X80764 mRNA. Translation: CAA56739.1.
X73960 mRNA. Translation: CAA52148.1.
AK052192 mRNA. Translation: BAC34876.1.
AK052413 mRNA. Translation: BAC34979.1.
AL627212 Genomic DNA. Translation: CAM15213.1.
BC046452 mRNA. Translation: AAH46452.2.
S79346 Genomic DNA. No translation available.
CCDSiCCDS18553.1.
PIRiJN0711.
RefSeqiNP_035717.2. NM_011587.2.
UniGeneiMm.4345.

Genome annotation databases

EnsembliENSMUST00000047421; ENSMUSP00000037129; ENSMUSG00000033191.
GeneIDi21846.
KEGGimmu:21846.
UCSCiuc008ukh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71425 mRNA. Translation: CAA50556.1.
X80764 mRNA. Translation: CAA56739.1.
X73960 mRNA. Translation: CAA52148.1.
AK052192 mRNA. Translation: BAC34876.1.
AK052413 mRNA. Translation: BAC34979.1.
AL627212 Genomic DNA. Translation: CAM15213.1.
BC046452 mRNA. Translation: AAH46452.2.
S79346 Genomic DNA. No translation available.
CCDSiCCDS18553.1.
PIRiJN0711.
RefSeqiNP_035717.2. NM_011587.2.
UniGeneiMm.4345.

3D structure databases

ProteinModelPortaliQ06806.
SMRiQ06806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ06806. 1 interactor.
MINTiMINT-8020582.
STRINGi10090.ENSMUSP00000037129.

Chemistry databases

BindingDBiQ06806.
ChEMBLiCHEMBL2034800.

PTM databases

iPTMnetiQ06806.
PhosphoSitePlusiQ06806.

Proteomic databases

MaxQBiQ06806.
PaxDbiQ06806.
PeptideAtlasiQ06806.
PRIDEiQ06806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047421; ENSMUSP00000037129; ENSMUSG00000033191.
GeneIDi21846.
KEGGimmu:21846.
UCSCiuc008ukh.1. mouse.

Organism-specific databases

CTDi7075.
MGIiMGI:99906. Tie1.

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOGENOMiHOG000049232.
HOVERGENiHBG007316.
InParanoidiQ06806.
KOiK05120.
OMAiHNSPGAH.
OrthoDBiEOG091G00RL.
TreeFamiTF317568.

Enzyme and pathway databases

BRENDAi2.7.10.1. 3474.

Miscellaneous databases

ChiTaRSiTie1. mouse.
PROiQ06806.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033191.
CleanExiMM_TIE1.
ExpressionAtlasiQ06806. baseline and differential.
GenevisibleiQ06806. MM.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF00047. ig. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00181. EGF. 3 hits.
SM00060. FN3. 3 hits.
SM00409. IG. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIE1_MOUSE
AccessioniPrimary (citable) accession number: Q06806
Secondary accession number(s): Q811F4, Q8BGI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.