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Q06801 (DPEP_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-alpha-glucanotransferase, chloroplastic/amyloplastic

EC=2.4.1.25
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme
Gene names
Name:DPEP
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act during starch breakdown to convert small oligosaccharides into larger molecules upon which starch phosphorylase can act, or may change the structure of starch molecules and grain architecture by modifying chain length, or may generate from starch and glucose oligosaccharides which can serve either as primers for new starch phosphoenzyme.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Subcellular location

Plastidchloroplast. Plastidamyloplast.

Tissue specificity

Present in leaves, stems, roots, and stolons but is most abundant in developing and mature tubers.

Sequence similarities

Belongs to the disproportionating enzyme family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentAmyloplast
Chloroplast
Plastid
   DomainTransit peptide
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentamyloplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

chloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4-alpha-glucanotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5252Chloroplast Ref.1
Chain53 – 5765244-alpha-glucanotransferase, chloroplastic/amyloplastic
PRO_0000018550

Secondary structure

................................................................................................. 576
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q06801 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: A0D16F3A546307BB

FASTA57664,951
        10         20         30         40         50         60 
MAIHTCFSLI PSSFSSPKLP YPKNTTFQSP IPKLSRPTFM FDRKGSFQNG TAAVPAVGED 

        70         80         90        100        110        120 
FPIDYADWLP KRDPNDRRRA GILLHPTSFP GPYGIGDLGP QAFKFLDWLH LAGCSLWQVL 

       130        140        150        160        170        180 
PLVPPGKRGN EDGSPYSGQD ANCGNTLLIS LEELVDDGLL KMEELPEPLP TDRVNYSTIS 

       190        200        210        220        230        240 
EIKDPLITKA AKRLLSSEGE LKDQLENFRR DPNISSWLED AAYFAAIDNS VNTISWYDWP 

       250        260        270        280        290        300 
EPLKNRHLAA LEEVYQSEKD FIDIFIAQQF LFQRQWKKVR DYARSKGISI MGDMPIYVGY 

       310        320        330        340        350        360 
HSADVWANKK QFLLNRKGFP LIVSGVPPDA FSETGQLWGS PLYDWKAMEK DGFSWWVRRI 

       370        380        390        400        410        420 
QRATDLFDEF RIDHFRGFAG FWAVPSEEKI AILGRWKVGP GKPLFDAILQ AVGKINIIAE 

       430        440        450        460        470        480 
DLGVITEDVV QLRKSIEAPG MAVLQFAFGS DAENPHLPHN HEQNQVVYTG THDNDTIRGW 

       490        500        510        520        530        540 
WDTLPQEEKS NVLKYLSNIE EEEISRGLIE GAVSSVARIA IIPMQDVLGL GSDSRMNIPA 

       550        560        570 
TQFGNWSWRI PSSTSFDNLD AEAKKLRDIL ATYGRL 

« Hide

References

[1]"Disproportionating enzyme (4-alpha-glucanotransferase; EC 2.4.1.25) of potato. Purification, molecular cloning, and potential role in starch metabolism."
Takaha T., Yanase M., Okada S., Smith S.M.
J. Biol. Chem. 268:1391-1396(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 53-57; 174-183 AND 247-259.
Strain: cv. May Queen.
Tissue: Tuber.
[2]"Structure determination and refinement at 1.8 A resolution of disproportionating enzyme from potato."
Imamura K., Matsuura T., Takaha T., Fujii K., Nakagawa A., Kusunoki M., Nitta Y.
Submitted (APR-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 53-576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68664 mRNA. Translation: CAA48630.1.
PIRA45049.
RefSeqNP_001274781.1. NM_001287852.1.
UniGeneStu.18047.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1X1NX-ray1.80A53-576[»]
ProteinModelPortalQ06801.
SMRQ06801. Positions 54-576.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH77. Glycoside Hydrolase Family 77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102595076.

Enzyme and pathway databases

BRENDA2.4.1.25. 5757.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR003385. Glyco_hydro_77.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02446. Glyco_hydro_77. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR00217. malQ. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ06801.

Entry information

Entry nameDPEP_SOLTU
AccessionPrimary (citable) accession number: Q06801
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references