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Q06801

- DPEP_SOLTU

UniProt

Q06801 - DPEP_SOLTU

Protein

4-alpha-glucanotransferase, chloroplastic/amyloplastic

Gene

DPEP

Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    May act during starch breakdown to convert small oligosaccharides into larger molecules upon which starch phosphorylase can act, or may change the structure of starch molecules and grain architecture by modifying chain length, or may generate from starch and glucose oligosaccharides which can serve either as primers for new starch phosphoenzyme.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BRENDAi2.4.1.25. 5757.

    Protein family/group databases

    CAZyiGH77. Glycoside Hydrolase Family 77.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-alpha-glucanotransferase, chloroplastic/amyloplastic (EC:2.4.1.25)
    Alternative name(s):
    Amylomaltase
    Disproportionating enzyme
    Short name:
    D-enzyme
    Gene namesi
    Name:DPEP
    OrganismiSolanum tuberosum (Potato)
    Taxonomic identifieri4113 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
    ProteomesiUP000011115: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252Chloroplast1 PublicationAdd
    BLAST
    Chaini53 – 5765244-alpha-glucanotransferase, chloroplastic/amyloplasticPRO_0000018550Add
    BLAST

    Expressioni

    Tissue specificityi

    Present in leaves, stems, roots, and stolons but is most abundant in developing and mature tubers.

    Structurei

    Secondary structure

    1
    576
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi65 – 673
    Helixi74 – 763
    Beta strandi79 – 835
    Helixi86 – 883
    Helixi100 – 11213
    Beta strandi116 – 1183
    Beta strandi128 – 1303
    Beta strandi137 – 1393
    Beta strandi141 – 1433
    Helixi146 – 1483
    Helixi151 – 1566
    Helixi162 – 1643
    Turni176 – 1783
    Helixi179 – 19618
    Helixi200 – 21011
    Helixi212 – 23120
    Helixi236 – 2383
    Helixi241 – 2444
    Helixi248 – 25710
    Helixi259 – 28527
    Beta strandi289 – 2979
    Beta strandi300 – 3023
    Helixi303 – 3064
    Helixi309 – 3113
    Beta strandi320 – 3267
    Beta strandi329 – 3335
    Helixi345 – 3506
    Helixi354 – 36613
    Beta strandi368 – 3736
    Helixi375 – 3784
    Beta strandi380 – 3856
    Beta strandi389 – 3935
    Beta strandi395 – 3984
    Helixi402 – 41211
    Beta strandi416 – 4194
    Helixi427 – 4359
    Beta strandi440 – 4434
    Helixi444 – 4463
    Beta strandi447 – 4504
    Helixi458 – 4603
    Beta strandi463 – 4697
    Helixi477 – 4826
    Helixi486 – 49510
    Helixi501 – 5033
    Helixi504 – 51310
    Beta strandi518 – 5236
    Helixi524 – 5274
    Helixi532 – 5343
    Beta strandi542 – 5443
    Helixi556 – 5583
    Helixi560 – 57213

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1X1NX-ray1.80A53-576[»]
    ProteinModelPortaliQ06801.
    SMRiQ06801. Positions 54-576.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ06801.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the disproportionating enzyme family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK00705.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR003385. Glyco_hydro_77.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02446. Glyco_hydro_77. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR00217. malQ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06801-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIHTCFSLI PSSFSSPKLP YPKNTTFQSP IPKLSRPTFM FDRKGSFQNG    50
    TAAVPAVGED FPIDYADWLP KRDPNDRRRA GILLHPTSFP GPYGIGDLGP 100
    QAFKFLDWLH LAGCSLWQVL PLVPPGKRGN EDGSPYSGQD ANCGNTLLIS 150
    LEELVDDGLL KMEELPEPLP TDRVNYSTIS EIKDPLITKA AKRLLSSEGE 200
    LKDQLENFRR DPNISSWLED AAYFAAIDNS VNTISWYDWP EPLKNRHLAA 250
    LEEVYQSEKD FIDIFIAQQF LFQRQWKKVR DYARSKGISI MGDMPIYVGY 300
    HSADVWANKK QFLLNRKGFP LIVSGVPPDA FSETGQLWGS PLYDWKAMEK 350
    DGFSWWVRRI QRATDLFDEF RIDHFRGFAG FWAVPSEEKI AILGRWKVGP 400
    GKPLFDAILQ AVGKINIIAE DLGVITEDVV QLRKSIEAPG MAVLQFAFGS 450
    DAENPHLPHN HEQNQVVYTG THDNDTIRGW WDTLPQEEKS NVLKYLSNIE 500
    EEEISRGLIE GAVSSVARIA IIPMQDVLGL GSDSRMNIPA TQFGNWSWRI 550
    PSSTSFDNLD AEAKKLRDIL ATYGRL 576
    Length:576
    Mass (Da):64,951
    Last modified:June 1, 1994 - v1
    Checksum:iA0D16F3A546307BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68664 mRNA. Translation: CAA48630.1.
    PIRiA45049.
    RefSeqiNP_001274781.1. NM_001287852.1.
    UniGeneiStu.18047.

    Genome annotation databases

    GeneIDi102595076.
    KEGGisot:102595076.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68664 mRNA. Translation: CAA48630.1 .
    PIRi A45049.
    RefSeqi NP_001274781.1. NM_001287852.1.
    UniGenei Stu.18047.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1X1N X-ray 1.80 A 53-576 [» ]
    ProteinModelPortali Q06801.
    SMRi Q06801. Positions 54-576.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH77. Glycoside Hydrolase Family 77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102595076.
    KEGGi sot:102595076.

    Phylogenomic databases

    KOi K00705.

    Enzyme and pathway databases

    BRENDAi 2.4.1.25. 5757.

    Miscellaneous databases

    EvolutionaryTracei Q06801.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR003385. Glyco_hydro_77.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02446. Glyco_hydro_77. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR00217. malQ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Disproportionating enzyme (4-alpha-glucanotransferase; EC 2.4.1.25) of potato. Purification, molecular cloning, and potential role in starch metabolism."
      Takaha T., Yanase M., Okada S., Smith S.M.
      J. Biol. Chem. 268:1391-1396(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 53-57; 174-183 AND 247-259.
      Strain: cv. May Queen.
      Tissue: Tuber.
    2. "Structure determination and refinement at 1.8 A resolution of disproportionating enzyme from potato."
      Imamura K., Matsuura T., Takaha T., Fujii K., Nakagawa A., Kusunoki M., Nitta Y.
      Submitted (APR-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 53-576.

    Entry informationi

    Entry nameiDPEP_SOLTU
    AccessioniPrimary (citable) accession number: Q06801
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3