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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291ZincBy similarity
Metal bindingi209 – 2091ZincBy similarity
Metal bindingi234 – 2341ZincBy similarity
Metal bindingi238 – 2381ZincBy similarity
Binding sitei269 – 2691ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU00940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Synonyms:spnA
Ordered Locus Names:BSU00940
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Cysteine--tRNA ligasePRO_0000159352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei270 – 2701Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ06752.

PTM databases

iPTMnetiQ06752.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiQ06752. 1 interaction.
MINTiMINT-8365725.
STRINGi224308.Bsubs1_010100000490.

Structurei

3D structure databases

ProteinModelPortaliQ06752.
SMRiQ06752. Positions 3-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi31 – 4111"HIGH" regionAdd
BLAST
Motifi266 – 2705"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiQ06752.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiQ06752.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITLYNTLT RQKETFVPLE EGKVKMYVCG PTVYNYIHIG NARPAIVYDT
60 70 80 90 100
VRNYLEYKGY DVQYVSNFTD VDDKLIKAAN ELGEDVPTIS ERFIKAYFED
110 120 130 140 150
VGALGCRKAD LHPRVMENMD AIIEFVDQLV KKGYAYESEG DVYFKTRAFE
160 170 180 190 200
GYGKLSQQSI DELRSGARIR VGEKKEDALD FALWKAAKEG EISWDSPWGK
210 220 230 240 250
GRPGWHIECS AMVKKYLGDQ IDIHAGGQDL TFPHHENEIA QSEALTGKTF
260 270 280 290 300
AKYWLHNGYI NIDNEKMSKS LGNFVLVHDI IKQHDPQLLR FFMLSVHYRH
310 320 330 340 350
PINYSEELLE NTKSAFSRLK TAYSNLQHRL NSSTNLTEDD DQWLEKVEEH
360 370 380 390 400
RKAFEEEMDD DFNTANAISV LFDLAKHANY YLQKDHTADH VITAFIEMFD
410 420 430 440 450
RIVSVLGFSL GEQELLDQEI EDLIEKRNEA RRNRDFALSD QIRDQLKSMN
460
IILEDTAQGT RWKRGE
Length:466
Mass (Da):53,908
Last modified:June 1, 1994 - v1
Checksum:i742D225C2E377CC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14580 Genomic DNA. Translation: AAA21798.1.
D26185 Genomic DNA. Translation: BAA05328.1.
X73989 Genomic DNA. Translation: CAA52167.1.
AL009126 Genomic DNA. Translation: CAB11870.1.
PIRiC53402.
RefSeqiNP_387975.1. NC_000964.3.
WP_004399682.1. NZ_JNCM01000029.1.

Genome annotation databases

EnsemblBacteriaiCAB11870; CAB11870; BSU00940.
GeneIDi936859.
KEGGibsu:BSU00940.
PATRICi18971697. VBIBacSub10457_0097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14580 Genomic DNA. Translation: AAA21798.1.
D26185 Genomic DNA. Translation: BAA05328.1.
X73989 Genomic DNA. Translation: CAA52167.1.
AL009126 Genomic DNA. Translation: CAB11870.1.
PIRiC53402.
RefSeqiNP_387975.1. NC_000964.3.
WP_004399682.1. NZ_JNCM01000029.1.

3D structure databases

ProteinModelPortaliQ06752.
SMRiQ06752. Positions 3-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ06752. 1 interaction.
MINTiMINT-8365725.
STRINGi224308.Bsubs1_010100000490.

PTM databases

iPTMnetiQ06752.

Proteomic databases

PaxDbiQ06752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11870; CAB11870; BSU00940.
GeneIDi936859.
KEGGibsu:BSU00940.
PATRICi18971697. VBIBacSub10457_0097.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiQ06752.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiQ06752.

Enzyme and pathway databases

BioCyciBSUB:BSU00940-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_BACSU
AccessioniPrimary (citable) accession number: Q06752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.