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Protein

Argininosuccinate synthase

Gene

argG

Organism
Streptomyces lavendulae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei99 – 991CitrullineBy similarity
Binding sitei129 – 1291ATP; via amide nitrogenBy similarity
Binding sitei131 – 1311AspartateBy similarity
Binding sitei131 – 1311ATPBy similarity
Binding sitei135 – 1351AspartateBy similarity
Binding sitei135 – 1351CitrullineBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361ATPBy similarity
Binding sitei139 – 1391CitrullineBy similarity
Binding sitei192 – 1921CitrullineBy similarity
Binding sitei194 – 1941ATPBy similarity
Binding sitei201 – 2011CitrullineBy similarity
Binding sitei203 – 2031CitrullineBy similarity
Binding sitei280 – 2801CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
OrganismiStreptomyces lavendulae
Taxonomic identifieri1914 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 482481Argininosuccinate synthasePRO_0000148709Add
BLAST

Proteomic databases

PRIDEiQ06734.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliQ06734.
SMRiQ06734. Positions 2-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06734-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKVLTSLPA GERVGIAFSG GLDTSVAVAW MRDKGAVPCT YTADIGQYDE
60 70 80 90 100
PDIASVPSRA SAYGAEITRL VDCRAALVEE GLAALACGAF HIRSGGRPYF
110 120 130 140 150
NTTPLGRAVT GTLLVRAMLE DGVQIWGDGS TFKGNDIERF YRYGLLANPH
160 170 180 190 200
LRIYKPWLDA DFVTELGGRK EMSEWLLAHG LPYRDSTEKA YSTDANIWGA
210 220 230 240 250
THEAKTLEHL DTGIETVDPI MGVRFWDPSV EIATEDVTVG FEQGRPVSIN
260 270 280 290 300
GKEFASAVDL VMEANAIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HIVYERLVNA IHNEDTLAAY HNEGRRLGRL MYEGRWLDPQ ALMIRESLQR
360 370 380 390 400
WVGTAVTGEV TLRLRRGEDY SILDTTGPAF SYHPDKLSME RTEDSAFGPV
410 420 430 440 450
DRIGQLTMRN LDIADSRARL EQYVGLGLVG TPHPTPIGAA QAAATGLIGA
460 470 480
MDEGGAEAIA SRGEATDEET MLDRAAMESG TD
Length:482
Mass (Da):52,383
Last modified:January 23, 2007 - v3
Checksum:iBFD58A4461B2137B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10691 Genomic DNA. Translation: BAA01533.1.
PIRiJN0506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10691 Genomic DNA. Translation: BAA01533.1.
PIRiJN0506.

3D structure databases

ProteinModelPortaliQ06734.
SMRiQ06734. Positions 2-431.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ06734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequence and expression of the argG gene from Streptomyces lavendulae."
    Ogawara H., Kasama H., Nashimoto K., Ohtsubo M., Higashi K., Urabe H.
    Gene 125:91-96(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10.
    Strain: KCC S0055.

Entry informationi

Entry nameiASSY_STRLA
AccessioniPrimary (citable) accession number: Q06734
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.