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Protein

Vacuole morphology and inheritance protein 14

Gene

VAC14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Regulates the synthesis of PtdIns(3,5)P2 by positive activation of FAB1 and by controlling FIG4 localization. Required for FIG4-mediated turnover of PtdIns(3,5)P2 after hyperosmotic shock. Essential for the control of trafficking of some proteins to the vacuole lumen via the multivesicular body (MVB), and for maintenance of vacuole size and acidity.5 Publications

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • phosphatidylinositol biosynthetic process Source: SGD
  • positive regulation of kinase activity Source: SGD
  • protein transport Source: UniProtKB-KW
  • regulation of lipid kinase activity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32452-MONOMER.
YEAST:G3O-32452-MONOMER.
ReactomeiR-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1660516. Synthesis of PIPs at the early endosome membrane.
R-SCE-1660517. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuole morphology and inheritance protein 14
Alternative name(s):
Swollen vacuole phenotype 2 protein
Gene namesi
Name:VAC14
Synonyms:SVP2
Ordered Locus Names:YLR386W
ORF Names:L3502.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR386W.
SGDiS000004378. VAC14.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of vacuolar membrane Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • PAS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56H → Y: Loss of interaction with ATG18 and VAC7. 1 Publication1
Mutagenesisi61R → K: Loss of interaction with ATG18 and VAC7. 1 Publication1
Mutagenesisi101Q → R: Loss of interaction with ATG18 and VAC7. 1 Publication1
Mutagenesisi149L → R: Loss of interaction with ATG18, FAB1 and VAC7. No loss of interaction with FIG4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657561 – 880Vacuole morphology and inheritance protein 14Add BLAST880

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei767PhosphoserineCombined sources1
Modified residuei805PhosphoserineCombined sources1
Modified residuei867PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06708.
PRIDEiQ06708.

PTM databases

iPTMnetiQ06708.

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-27189,EBI-27189
ATG18P436015EBI-27189,EBI-22968
FAB1P347569EBI-27189,EBI-6754
FIG4P428377EBI-27189,EBI-28407
VAC7P539504EBI-27189,EBI-28714

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi31645. 178 interactors.
DIPiDIP-1176N.
IntActiQ06708. 26 interactors.
MINTiMINT-401035.

Structurei

3D structure databases

ProteinModelPortaliQ06708.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati82 – 119HEAT 1Add BLAST38
Repeati243 – 280HEAT 2Add BLAST38
Repeati388 – 425HEAT 3Add BLAST38
Repeati429 – 466HEAT 4Add BLAST38
Repeati517 – 554HEAT 5Add BLAST38

Domaini

The N-terminal domain mediates interaction with FAB1 and VAC7 while the C-terminal domain mediates interaction with FIG4.1 Publication

Sequence similaritiesi

Belongs to the VAC14 family.Curated
Contains 5 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000008385.
HOGENOMiHOG000216640.
InParanoidiQ06708.
KOiK15305.
OMAiCIEKEED.
OrthoDBiEOG092C0MJZ.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR026825. Vac14.
IPR032878. Vac14_Fab1-bd.
IPR021841. VAC14_Fig4p-bd.
[Graphical view]
PANTHERiPTHR16023. PTHR16023. 3 hits.
PfamiPF12755. Vac14_Fab1_bd. 1 hit.
PF11916. Vac14_Fig4_bd. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKSIAKGLS DKLYEKRKAA ALELEKLVKQ CVLEGDYDRI DKIIDELCRD
60 70 80 90 100
YAYALHQPMA RNAGLMGLAA TAIALGINDV GRYLRNILPP VLACFGDQND
110 120 130 140 150
QVRFYACESL YNIAKIAKGE ILVYFNEIFD VLCKISADTE NSVRGAAELL
160 170 180 190 200
DRLIKDIVAE RASNYISIVN NGSHGLLPAI KTDPISGDVY QEEYEQDNQL
210 220 230 240 250
AFSLPKFIPL LTERIYAINP DTRVFLVDWL KVLLNTPGLE LISYLPSFLG
260 270 280 290 300
GLFTFLGDSH KDVRTVTHTL MDSLLHEVDR ISKLQTEIKM KRLERLKMLE
310 320 330 340 350
DKYNNSSTPT KKADGALIAE KKKTLMTALG GLSKPLSMET DDTKLSNTNE
360 370 380 390 400
TDDERHLTSQ EQLLDSEATS QEPLRDGEEY IPGQDINLNF PEVITVLVNN
410 420 430 440 450
LASSEAEIQL IALHWIQVIL SISPNVFIPF LSKILSVLLK LLSDSDPHIT
460 470 480 490 500
EIAQLVNGQL LSLCSSYVGK ETDGKIAYGP IVNSLTLQFF DSRIDAKIAC
510 520 530 540 550
LDWLILIYHK APNQILKHND SMFLTLLKSL SNRDSVLIEK ALSLLQSLCS
560 570 580 590 600
DSNDNYLRQF LQDLLTLFKR DTKLVKTRAN FIMRQISSRL SPERVYKVIS
610 620 630 640 650
SILDNYNDTT FVKMMIQILS TNLITSPEMS SLRNKLRTCE DGMFFNSLFK
660 670 680 690 700
SWCPNPVSVI SLCFVAENYE LAYTVLQTYA NYELKLNDLV QLDILIQLFE
710 720 730 740 750
SPVFTRMRLQ LLEQQKHPFL HKCLFGILMI IPQSKAFETL NRRLNSLNIW
760 770 780 790 800
TSQSYVMNNY IRQRENSNFC DSNSDISQRS VSQSKLHFQE LINHFKAVSE
810 820 830 840 850
EDEYSSDMIR LDHGANNKSL LLGSFLDGID EDKQEIVTPI SPMNEAINEE
860 870 880
MESPNDNSSV ILKDSGSLPF NRNVSDKLKK
Length:880
Mass (Da):99,773
Last modified:November 1, 1996 - v1
Checksum:iE625AB6F032D2E1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19104 Genomic DNA. Translation: AAB67272.1.
BK006945 Genomic DNA. Translation: DAA09687.1.
PIRiS51473.
RefSeqiNP_013490.3. NM_001182275.3.

Genome annotation databases

EnsemblFungiiYLR386W; YLR386W; YLR386W.
GeneIDi851102.
KEGGisce:YLR386W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19104 Genomic DNA. Translation: AAB67272.1.
BK006945 Genomic DNA. Translation: DAA09687.1.
PIRiS51473.
RefSeqiNP_013490.3. NM_001182275.3.

3D structure databases

ProteinModelPortaliQ06708.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31645. 178 interactors.
DIPiDIP-1176N.
IntActiQ06708. 26 interactors.
MINTiMINT-401035.

PTM databases

iPTMnetiQ06708.

Proteomic databases

MaxQBiQ06708.
PRIDEiQ06708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR386W; YLR386W; YLR386W.
GeneIDi851102.
KEGGisce:YLR386W.

Organism-specific databases

EuPathDBiFungiDB:YLR386W.
SGDiS000004378. VAC14.

Phylogenomic databases

GeneTreeiENSGT00390000008385.
HOGENOMiHOG000216640.
InParanoidiQ06708.
KOiK15305.
OMAiCIEKEED.
OrthoDBiEOG092C0MJZ.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32452-MONOMER.
YEAST:G3O-32452-MONOMER.
ReactomeiR-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1660516. Synthesis of PIPs at the early endosome membrane.
R-SCE-1660517. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

PROiQ06708.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR026825. Vac14.
IPR032878. Vac14_Fab1-bd.
IPR021841. VAC14_Fig4p-bd.
[Graphical view]
PANTHERiPTHR16023. PTHR16023. 3 hits.
PfamiPF12755. Vac14_Fab1_bd. 1 hit.
PF11916. Vac14_Fig4_bd. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAC14_YEAST
AccessioniPrimary (citable) accession number: Q06708
Secondary accession number(s): D6VZ21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12388 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.