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Q06706

- ELP1_YEAST

UniProt

Q06706 - ELP1_YEAST

Protein

Elongator complex protein 1

Gene

IKI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. Independently, ELP3 may be involved in polarized exocytosis. It is required for the polarized localization of GTPase-activating protein SEC2. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.8 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. tRNA binding Source: SGD

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. regulation of transcription from RNA polymerase II promoter Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW
    4. tRNA wobble uridine modification Source: SGD

    Keywords - Biological processi

    Protein transport, Transcription, Transcription regulation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32450-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongator complex protein 1
    Alternative name(s):
    Gamma-toxin target 1
    Protein IKI3
    Gene namesi
    Name:IKI3
    Synonyms:ELP1, TOT1
    Ordered Locus Names:YLR384C
    ORF Names:L3502.7
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR384c.
    SGDiS000004376. IKI3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. Elongator holoenzyme complex Source: SGD
    3. nucleoplasm Source: Reactome
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13491349Elongator complex protein 1PRO_0000084179Add
    BLAST

    Proteomic databases

    MaxQBiQ06706.
    PaxDbiQ06706.
    PeptideAtlasiQ06706.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06706.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, IKI3 interacts with ELP2.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ELP4Q028849EBI-9068,EBI-35277
    IKI1P388748EBI-9068,EBI-9061

    Protein-protein interaction databases

    BioGridi31643. 259 interactions.
    DIPiDIP-2384N.
    IntActiQ06706. 19 interactions.
    MINTiMINT-567397.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06706.
    SMRiQ06706. Positions 144-170, 356-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ELP1/IKA1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5290.
    GeneTreeiENSGT00390000013344.
    HOGENOMiHOG000188454.
    KOiK11373.
    OMAiTREVKNE.
    OrthoDBiEOG7673K1.

    Family and domain databases

    Gene3Di2.130.10.10. 3 hits.
    InterProiIPR006849. IKI3.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PANTHERiPTHR12747. PTHR12747. 1 hit.
    PfamiPF04762. IKI3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017233. IKAP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q06706-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEHDKSGSK RQELRSNMRN LITLNKGKFK PTASTAEGDE DDLSFTLLDS     50
    VFDTLSDSIT CVLGSTDIGA IEVQQFMKDG SRNVLASFNI QTFDDKLLSF 100
    VHFADINQLV FVFEQGDIIT ATYDPVSLDP AETLIEIMGT IDNGIAAAQW 150
    SYDEETLAMV TKDRNVVVLS KLFEPISEYH LEVDDLKISK HVTVGWGKKE 200
    TQFRGKGARA MEREALASLK ASGLVGNQLR DPTMPYMVDT GDVTALDSHE 250
    ITISWRGDCD YFAVSSVEEV PDEDDETKSI KRRAFRVFSR EGQLDSASEP 300
    VTGMEHQLSW KPQGSLIASI QRKTDLGEED SVDVIFFERN GLRHGEFDTR 350
    LPLDEKVESV CWNSNSEALA VVLANRIQLW TSKNYHWYLK QELYASDISY 400
    VKWHPEKDFT LMFSDAGFIN IVDFAYKMAQ GPTLEPFDNG TSLVVDGRTV 450
    NITPLALANV PPPMYYRDFE TPGNVLDVAC SFSNEIYAAI NKDVLIFAAV 500
    PSIEEMKKGK HPSIVCEFPK SEFTSEVDSL RQVAFINDSI VGVLLDTDNL 550
    SRIALLDIQD ITQPTLITIV EVYDKIVLLR SDFDYNHLVY ETRDGTVCQL 600
    DAEGQLMEIT KFPQLVRDFR VKRVHNTSAE DDDNWSAESS ELVAFGITNN 650
    GKLFANQVLL ASAVTSLEIT DSFLLFTTAQ HNLQFVHLNS TDFKPLPLVE 700
    EGVEDERVRA IERGSILVSV IPSKSSVVLQ ATRGNLETIY PRIMVLAEVR 750
    KNIMAKRYKE AFIVCRTHRI NLDILHDYAP ELFIENLEVF INQIGRVDYL 800
    NLFISCLSED DVTKTKYKET LYSGISKSFG MEPAPLTEMQ IYMKKKMFDP 850
    KTSKVNKICD AVLNVLLSNP EYKKKYLQTI ITAYASQNPQ NLSAALKLIS 900
    ELENSEEKDS CVTYLCFLQD VNVVYKSALS LYDVSLALLV AQKSQMDPRE 950
    YLPFLQELQD NEPLRRKFLI DDYLGNYEKA LEHLSEIDKD GNVSEEVIDY 1000
    VESHDLYKHG LALYRYDSEK QNVIYNIYAK HLSSNQMYTD AAVAYEMLGK 1050
    LKEAMGAYQS AKRWREAMSI AVQKFPEEVE SVAEELISSL TFEHRYVDAA 1100
    DIQLEYLDNV KEAVALYCKA YRYDIASLVA IKAKKDELLE EVVDPGLGEG 1150
    FGIIAELLAD CKGQINSQLR RLRELRAKKE ENPYAFYGQE TEQADDVSVA 1200
    PSETSTQESF FTRYTGKTGG TAKTGASRRT AKNKRREERK RARGKKGTIY 1250
    EEEYLVQSVG RLIERLNQTK PDAVRVVEGL CRRNMREQAH QIQKNFVEVL 1300
    DLLKANVKEI YSISEKDRER VNENGEVYYI PEIPVPEIHD FPKSHIVDF 1349
    Length:1,349
    Mass (Da):152,990
    Last modified:November 1, 1996 - v1
    Checksum:i7AEB1C0BA99282F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87841 Genomic DNA. Translation: BAA20120.1.
    U19104 Genomic DNA. Translation: AAB67278.1.
    BK006945 Genomic DNA. Translation: DAA09685.1.
    PIRiS51471.
    RefSeqiNP_013488.3. NM_001182273.3.

    Genome annotation databases

    EnsemblFungiiYLR384C; YLR384C; YLR384C.
    GeneIDi851100.
    KEGGisce:YLR384C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87841 Genomic DNA. Translation: BAA20120.1 .
    U19104 Genomic DNA. Translation: AAB67278.1 .
    BK006945 Genomic DNA. Translation: DAA09685.1 .
    PIRi S51471.
    RefSeqi NP_013488.3. NM_001182273.3.

    3D structure databases

    ProteinModelPortali Q06706.
    SMRi Q06706. Positions 144-170, 356-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31643. 259 interactions.
    DIPi DIP-2384N.
    IntActi Q06706. 19 interactions.
    MINTi MINT-567397.

    Proteomic databases

    MaxQBi Q06706.
    PaxDbi Q06706.
    PeptideAtlasi Q06706.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR384C ; YLR384C ; YLR384C .
    GeneIDi 851100.
    KEGGi sce:YLR384C.

    Organism-specific databases

    CYGDi YLR384c.
    SGDi S000004376. IKI3.

    Phylogenomic databases

    eggNOGi COG5290.
    GeneTreei ENSGT00390000013344.
    HOGENOMi HOG000188454.
    KOi K11373.
    OMAi TREVKNE.
    OrthoDBi EOG7673K1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32450-MONOMER.

    Miscellaneous databases

    NextBioi 967788.
    PROi Q06706.

    Gene expression databases

    Genevestigatori Q06706.

    Family and domain databases

    Gene3Di 2.130.10.10. 3 hits.
    InterProi IPR006849. IKI3.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    PANTHERi PTHR12747. PTHR12747. 1 hit.
    Pfami PF04762. IKI3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017233. IKAP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
      Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
      Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
      Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
    5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
      Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
      EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
    6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
      Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
    7. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
      Krogan N.J., Greenblatt J.F.
      Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
    8. "Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
      Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
      Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ELP2.
    9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
      Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
    10. "Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
      Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
      J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
      Klassen R., Meinhardt F.
      Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
      Rahl P.B., Chen C.Z., Collins R.N.
      Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN EXOCYTOSIS REGULATION, INTERACTION WITH SEC2, SUBCELLULAR LOCATION.
    15. "An early step in wobble uridine tRNA modification requires the Elongator complex."
      Huang B., Johansson M.J.O., Bystroem A.S.
      RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRNA MODIFICATION.

    Entry informationi

    Entry nameiELP1_YEAST
    AccessioniPrimary (citable) accession number: Q06706
    Secondary accession number(s): D6VZ19, O00036
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3