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Q06706 (ELP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongator complex protein 1
Alternative name(s):
Gamma-toxin target 1
Protein IKI3
Gene names
Name:IKI3
Synonyms:ELP1, TOT1
Ordered Locus Names:YLR384C
ORF Names:L3502.7
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. Independently, ELP3 may be involved in polarized exocytosis. It is required for the polarized localization of GTPase-activating protein SEC2. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Ref.4 Ref.5 Ref.7 Ref.9 Ref.10 Ref.11 Ref.14 Ref.15

Subunit structure

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, IKI3 interacts with ELP2. Ref.6 Ref.7 Ref.8 Ref.14

Subcellular location

Cytoplasm. Nucleus Ref.4 Ref.12 Ref.14.

Miscellaneous

Present with 10500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ELP1/IKA1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ELP4Q028849EBI-9068,EBI-35277
IKI1P388748EBI-9068,EBI-9061

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13491349Elongator complex protein 1
PRO_0000084179

Sequences

Sequence LengthMass (Da)Tools
Q06706 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7AEB1C0BA99282F6

FASTA1,349152,990
        10         20         30         40         50         60 
MVEHDKSGSK RQELRSNMRN LITLNKGKFK PTASTAEGDE DDLSFTLLDS VFDTLSDSIT 

        70         80         90        100        110        120 
CVLGSTDIGA IEVQQFMKDG SRNVLASFNI QTFDDKLLSF VHFADINQLV FVFEQGDIIT 

       130        140        150        160        170        180 
ATYDPVSLDP AETLIEIMGT IDNGIAAAQW SYDEETLAMV TKDRNVVVLS KLFEPISEYH 

       190        200        210        220        230        240 
LEVDDLKISK HVTVGWGKKE TQFRGKGARA MEREALASLK ASGLVGNQLR DPTMPYMVDT 

       250        260        270        280        290        300 
GDVTALDSHE ITISWRGDCD YFAVSSVEEV PDEDDETKSI KRRAFRVFSR EGQLDSASEP 

       310        320        330        340        350        360 
VTGMEHQLSW KPQGSLIASI QRKTDLGEED SVDVIFFERN GLRHGEFDTR LPLDEKVESV 

       370        380        390        400        410        420 
CWNSNSEALA VVLANRIQLW TSKNYHWYLK QELYASDISY VKWHPEKDFT LMFSDAGFIN 

       430        440        450        460        470        480 
IVDFAYKMAQ GPTLEPFDNG TSLVVDGRTV NITPLALANV PPPMYYRDFE TPGNVLDVAC 

       490        500        510        520        530        540 
SFSNEIYAAI NKDVLIFAAV PSIEEMKKGK HPSIVCEFPK SEFTSEVDSL RQVAFINDSI 

       550        560        570        580        590        600 
VGVLLDTDNL SRIALLDIQD ITQPTLITIV EVYDKIVLLR SDFDYNHLVY ETRDGTVCQL 

       610        620        630        640        650        660 
DAEGQLMEIT KFPQLVRDFR VKRVHNTSAE DDDNWSAESS ELVAFGITNN GKLFANQVLL 

       670        680        690        700        710        720 
ASAVTSLEIT DSFLLFTTAQ HNLQFVHLNS TDFKPLPLVE EGVEDERVRA IERGSILVSV 

       730        740        750        760        770        780 
IPSKSSVVLQ ATRGNLETIY PRIMVLAEVR KNIMAKRYKE AFIVCRTHRI NLDILHDYAP 

       790        800        810        820        830        840 
ELFIENLEVF INQIGRVDYL NLFISCLSED DVTKTKYKET LYSGISKSFG MEPAPLTEMQ 

       850        860        870        880        890        900 
IYMKKKMFDP KTSKVNKICD AVLNVLLSNP EYKKKYLQTI ITAYASQNPQ NLSAALKLIS 

       910        920        930        940        950        960 
ELENSEEKDS CVTYLCFLQD VNVVYKSALS LYDVSLALLV AQKSQMDPRE YLPFLQELQD 

       970        980        990       1000       1010       1020 
NEPLRRKFLI DDYLGNYEKA LEHLSEIDKD GNVSEEVIDY VESHDLYKHG LALYRYDSEK 

      1030       1040       1050       1060       1070       1080 
QNVIYNIYAK HLSSNQMYTD AAVAYEMLGK LKEAMGAYQS AKRWREAMSI AVQKFPEEVE 

      1090       1100       1110       1120       1130       1140 
SVAEELISSL TFEHRYVDAA DIQLEYLDNV KEAVALYCKA YRYDIASLVA IKAKKDELLE 

      1150       1160       1170       1180       1190       1200 
EVVDPGLGEG FGIIAELLAD CKGQINSQLR RLRELRAKKE ENPYAFYGQE TEQADDVSVA 

      1210       1220       1230       1240       1250       1260 
PSETSTQESF FTRYTGKTGG TAKTGASRRT AKNKRREERK RARGKKGTIY EEEYLVQSVG 

      1270       1280       1290       1300       1310       1320 
RLIERLNQTK PDAVRVVEGL CRRNMREQAH QIQKNFVEVL DLLKANVKEI YSISEKDRER 

      1330       1340 
VNENGEVYYI PEIPVPEIHD FPKSHIVDF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
[5]"Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
[6]"RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
[7]"Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
Krogan N.J., Greenblatt J.F.
Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
[8]"Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ELP2.
[9]"Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
[10]"Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
Klassen R., Meinhardt F.
Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
[12]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
Rahl P.B., Chen C.Z., Collins R.N.
Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN EXOCYTOSIS REGULATION, INTERACTION WITH SEC2, SUBCELLULAR LOCATION.
[15]"An early step in wobble uridine tRNA modification requires the Elongator complex."
Huang B., Johansson M.J.O., Bystroem A.S.
RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN TRNA MODIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87841 Genomic DNA. Translation: BAA20120.1.
U19104 Genomic DNA. Translation: AAB67278.1.
BK006945 Genomic DNA. Translation: DAA09685.1.
PIRS51471.
RefSeqNP_013488.3. NM_001182273.3.

3D structure databases

ProteinModelPortalQ06706.
SMRQ06706. Positions 144-169, 355-423, 471-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31643. 257 interactions.
DIPDIP-2384N.
IntActQ06706. 19 interactions.
MINTMINT-567397.

Proteomic databases

PaxDbQ06706.
PeptideAtlasQ06706.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR384C; YLR384C; YLR384C.
GeneID851100.
KEGGsce:YLR384C.

Organism-specific databases

CYGDYLR384c.
SGDS000004376. IKI3.

Phylogenomic databases

eggNOGCOG5290.
GeneTreeENSGT00390000013344.
HOGENOMHOG000188454.
KOK11373.
OMATREVKNE.
OrthoDBEOG7673K1.

Enzyme and pathway databases

BioCycYEAST:G3O-32450-MONOMER.

Gene expression databases

GenevestigatorQ06706.

Family and domain databases

Gene3D2.130.10.10. 3 hits.
InterProIPR006849. IKI3.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERPTHR12747. PTHR12747. 1 hit.
PfamPF04762. IKI3. 1 hit.
[Graphical view]
PIRSFPIRSF017233. IKAP. 1 hit.
ProtoNetSearch...

Other

NextBio967788.
PROQ06706.

Entry information

Entry nameELP1_YEAST
AccessionPrimary (citable) accession number: Q06706
Secondary accession number(s): D6VZ19, O00036
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families