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Q06706

- ELP1_YEAST

UniProt

Q06706 - ELP1_YEAST

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Protein

Elongator complex protein 1

Gene

IKI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. Independently, ELP3 may be involved in polarized exocytosis. It is required for the polarized localization of GTPase-activating protein SEC2. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.8 Publications

GO - Molecular functioni

  1. tRNA binding Source: SGD

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. regulation of transcription from RNA polymerase II promoter Source: SGD
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 1
Alternative name(s):
Gamma-toxin target 1
Protein IKI3
Gene namesi
Name:IKI3
Synonyms:ELP1, TOT1
Ordered Locus Names:YLR384C
ORF Names:L3502.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR384c.
SGDiS000004376. IKI3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. Elongator holoenzyme complex Source: SGD
  3. nucleoplasm Source: Reactome
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13491349Elongator complex protein 1PRO_0000084179Add
BLAST

Proteomic databases

MaxQBiQ06706.
PaxDbiQ06706.
PeptideAtlasiQ06706.

Expressioni

Gene expression databases

GenevestigatoriQ06706.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, IKI3 interacts with ELP2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP4Q028849EBI-9068,EBI-35277
IKI1P388748EBI-9068,EBI-9061

Protein-protein interaction databases

BioGridi31643. 260 interactions.
DIPiDIP-2384N.
IntActiQ06706. 19 interactions.
MINTiMINT-567397.

Structurei

3D structure databases

ProteinModelPortaliQ06706.
SMRiQ06706. Positions 143-168, 661-686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP1/IKA1 family.Curated

Phylogenomic databases

eggNOGiCOG5290.
GeneTreeiENSGT00390000013344.
HOGENOMiHOG000188454.
InParanoidiQ06706.
KOiK11373.
OMAiTREVKNE.
OrthoDBiEOG7673K1.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR006849. IKI3.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR12747. PTHR12747. 1 hit.
PfamiPF04762. IKI3. 1 hit.
[Graphical view]
PIRSFiPIRSF017233. IKAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06706-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEHDKSGSK RQELRSNMRN LITLNKGKFK PTASTAEGDE DDLSFTLLDS
60 70 80 90 100
VFDTLSDSIT CVLGSTDIGA IEVQQFMKDG SRNVLASFNI QTFDDKLLSF
110 120 130 140 150
VHFADINQLV FVFEQGDIIT ATYDPVSLDP AETLIEIMGT IDNGIAAAQW
160 170 180 190 200
SYDEETLAMV TKDRNVVVLS KLFEPISEYH LEVDDLKISK HVTVGWGKKE
210 220 230 240 250
TQFRGKGARA MEREALASLK ASGLVGNQLR DPTMPYMVDT GDVTALDSHE
260 270 280 290 300
ITISWRGDCD YFAVSSVEEV PDEDDETKSI KRRAFRVFSR EGQLDSASEP
310 320 330 340 350
VTGMEHQLSW KPQGSLIASI QRKTDLGEED SVDVIFFERN GLRHGEFDTR
360 370 380 390 400
LPLDEKVESV CWNSNSEALA VVLANRIQLW TSKNYHWYLK QELYASDISY
410 420 430 440 450
VKWHPEKDFT LMFSDAGFIN IVDFAYKMAQ GPTLEPFDNG TSLVVDGRTV
460 470 480 490 500
NITPLALANV PPPMYYRDFE TPGNVLDVAC SFSNEIYAAI NKDVLIFAAV
510 520 530 540 550
PSIEEMKKGK HPSIVCEFPK SEFTSEVDSL RQVAFINDSI VGVLLDTDNL
560 570 580 590 600
SRIALLDIQD ITQPTLITIV EVYDKIVLLR SDFDYNHLVY ETRDGTVCQL
610 620 630 640 650
DAEGQLMEIT KFPQLVRDFR VKRVHNTSAE DDDNWSAESS ELVAFGITNN
660 670 680 690 700
GKLFANQVLL ASAVTSLEIT DSFLLFTTAQ HNLQFVHLNS TDFKPLPLVE
710 720 730 740 750
EGVEDERVRA IERGSILVSV IPSKSSVVLQ ATRGNLETIY PRIMVLAEVR
760 770 780 790 800
KNIMAKRYKE AFIVCRTHRI NLDILHDYAP ELFIENLEVF INQIGRVDYL
810 820 830 840 850
NLFISCLSED DVTKTKYKET LYSGISKSFG MEPAPLTEMQ IYMKKKMFDP
860 870 880 890 900
KTSKVNKICD AVLNVLLSNP EYKKKYLQTI ITAYASQNPQ NLSAALKLIS
910 920 930 940 950
ELENSEEKDS CVTYLCFLQD VNVVYKSALS LYDVSLALLV AQKSQMDPRE
960 970 980 990 1000
YLPFLQELQD NEPLRRKFLI DDYLGNYEKA LEHLSEIDKD GNVSEEVIDY
1010 1020 1030 1040 1050
VESHDLYKHG LALYRYDSEK QNVIYNIYAK HLSSNQMYTD AAVAYEMLGK
1060 1070 1080 1090 1100
LKEAMGAYQS AKRWREAMSI AVQKFPEEVE SVAEELISSL TFEHRYVDAA
1110 1120 1130 1140 1150
DIQLEYLDNV KEAVALYCKA YRYDIASLVA IKAKKDELLE EVVDPGLGEG
1160 1170 1180 1190 1200
FGIIAELLAD CKGQINSQLR RLRELRAKKE ENPYAFYGQE TEQADDVSVA
1210 1220 1230 1240 1250
PSETSTQESF FTRYTGKTGG TAKTGASRRT AKNKRREERK RARGKKGTIY
1260 1270 1280 1290 1300
EEEYLVQSVG RLIERLNQTK PDAVRVVEGL CRRNMREQAH QIQKNFVEVL
1310 1320 1330 1340
DLLKANVKEI YSISEKDRER VNENGEVYYI PEIPVPEIHD FPKSHIVDF
Length:1,349
Mass (Da):152,990
Last modified:November 1, 1996 - v1
Checksum:i7AEB1C0BA99282F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87841 Genomic DNA. Translation: BAA20120.1.
U19104 Genomic DNA. Translation: AAB67278.1.
BK006945 Genomic DNA. Translation: DAA09685.1.
PIRiS51471.
RefSeqiNP_013488.3. NM_001182273.3.

Genome annotation databases

EnsemblFungiiYLR384C; YLR384C; YLR384C.
GeneIDi851100.
KEGGisce:YLR384C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87841 Genomic DNA. Translation: BAA20120.1 .
U19104 Genomic DNA. Translation: AAB67278.1 .
BK006945 Genomic DNA. Translation: DAA09685.1 .
PIRi S51471.
RefSeqi NP_013488.3. NM_001182273.3.

3D structure databases

ProteinModelPortali Q06706.
SMRi Q06706. Positions 143-168, 661-686.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31643. 260 interactions.
DIPi DIP-2384N.
IntActi Q06706. 19 interactions.
MINTi MINT-567397.

Proteomic databases

MaxQBi Q06706.
PaxDbi Q06706.
PeptideAtlasi Q06706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR384C ; YLR384C ; YLR384C .
GeneIDi 851100.
KEGGi sce:YLR384C.

Organism-specific databases

CYGDi YLR384c.
SGDi S000004376. IKI3.

Phylogenomic databases

eggNOGi COG5290.
GeneTreei ENSGT00390000013344.
HOGENOMi HOG000188454.
InParanoidi Q06706.
KOi K11373.
OMAi TREVKNE.
OrthoDBi EOG7673K1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32450-MONOMER.

Miscellaneous databases

NextBioi 967788.
PROi Q06706.

Gene expression databases

Genevestigatori Q06706.

Family and domain databases

Gene3Di 2.130.10.10. 3 hits.
InterProi IPR006849. IKI3.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
PANTHERi PTHR12747. PTHR12747. 1 hit.
Pfami PF04762. IKI3. 1 hit.
[Graphical view ]
PIRSFi PIRSF017233. IKAP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
    Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
    Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
    Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
  5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
    Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
    EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
  6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
    Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
  7. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
    Krogan N.J., Greenblatt J.F.
    Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
  8. "Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
    Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
    Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ELP2.
  9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
    Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
  10. "Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
    Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
    J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
    Klassen R., Meinhardt F.
    Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
    Rahl P.B., Chen C.Z., Collins R.N.
    Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS REGULATION, INTERACTION WITH SEC2, SUBCELLULAR LOCATION.
  15. "An early step in wobble uridine tRNA modification requires the Elongator complex."
    Huang B., Johansson M.J.O., Bystroem A.S.
    RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRNA MODIFICATION.

Entry informationi

Entry nameiELP1_YEAST
AccessioniPrimary (citable) accession number: Q06706
Secondary accession number(s): D6VZ19, O00036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3