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Q06706

- ELP1_YEAST

UniProt

Q06706 - ELP1_YEAST

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Protein
Elongator complex protein 1
Gene
IKI3, ELP1, TOT1, YLR384C, L3502.7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. Independently, ELP3 may be involved in polarized exocytosis. It is required for the polarized localization of GTPase-activating protein SEC2. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.8 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. tRNA binding Source: SGD

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. regulation of transcription from RNA polymerase II promoter Source: SGD
  3. tRNA wobble uridine modification Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 1
Alternative name(s):
Gamma-toxin target 1
Protein IKI3
Gene namesi
Name:IKI3
Synonyms:ELP1, TOT1
Ordered Locus Names:YLR384C
ORF Names:L3502.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR384c.
SGDiS000004376. IKI3.

Subcellular locationi

Cytoplasm. Nucleus 3 Publications

GO - Cellular componenti

  1. Elongator holoenzyme complex Source: SGD
  2. cytoplasm Source: SGD
  3. nucleoplasm Source: Reactome
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13491349Elongator complex protein 1
PRO_0000084179Add
BLAST

Proteomic databases

MaxQBiQ06706.
PaxDbiQ06706.
PeptideAtlasiQ06706.

Expressioni

Gene expression databases

GenevestigatoriQ06706.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, IKI3 interacts with ELP2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP4Q028849EBI-9068,EBI-35277
IKI1P388748EBI-9068,EBI-9061

Protein-protein interaction databases

BioGridi31643. 258 interactions.
DIPiDIP-2384N.
IntActiQ06706. 19 interactions.
MINTiMINT-567397.

Structurei

3D structure databases

ProteinModelPortaliQ06706.
SMRiQ06706. Positions 144-170, 356-382.

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP1/IKA1 family.

Phylogenomic databases

eggNOGiCOG5290.
GeneTreeiENSGT00390000013344.
HOGENOMiHOG000188454.
KOiK11373.
OMAiTREVKNE.
OrthoDBiEOG7673K1.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR006849. IKI3.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR12747. PTHR12747. 1 hit.
PfamiPF04762. IKI3. 1 hit.
[Graphical view]
PIRSFiPIRSF017233. IKAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06706-1 [UniParc]FASTAAdd to Basket

« Hide

MVEHDKSGSK RQELRSNMRN LITLNKGKFK PTASTAEGDE DDLSFTLLDS     50
VFDTLSDSIT CVLGSTDIGA IEVQQFMKDG SRNVLASFNI QTFDDKLLSF 100
VHFADINQLV FVFEQGDIIT ATYDPVSLDP AETLIEIMGT IDNGIAAAQW 150
SYDEETLAMV TKDRNVVVLS KLFEPISEYH LEVDDLKISK HVTVGWGKKE 200
TQFRGKGARA MEREALASLK ASGLVGNQLR DPTMPYMVDT GDVTALDSHE 250
ITISWRGDCD YFAVSSVEEV PDEDDETKSI KRRAFRVFSR EGQLDSASEP 300
VTGMEHQLSW KPQGSLIASI QRKTDLGEED SVDVIFFERN GLRHGEFDTR 350
LPLDEKVESV CWNSNSEALA VVLANRIQLW TSKNYHWYLK QELYASDISY 400
VKWHPEKDFT LMFSDAGFIN IVDFAYKMAQ GPTLEPFDNG TSLVVDGRTV 450
NITPLALANV PPPMYYRDFE TPGNVLDVAC SFSNEIYAAI NKDVLIFAAV 500
PSIEEMKKGK HPSIVCEFPK SEFTSEVDSL RQVAFINDSI VGVLLDTDNL 550
SRIALLDIQD ITQPTLITIV EVYDKIVLLR SDFDYNHLVY ETRDGTVCQL 600
DAEGQLMEIT KFPQLVRDFR VKRVHNTSAE DDDNWSAESS ELVAFGITNN 650
GKLFANQVLL ASAVTSLEIT DSFLLFTTAQ HNLQFVHLNS TDFKPLPLVE 700
EGVEDERVRA IERGSILVSV IPSKSSVVLQ ATRGNLETIY PRIMVLAEVR 750
KNIMAKRYKE AFIVCRTHRI NLDILHDYAP ELFIENLEVF INQIGRVDYL 800
NLFISCLSED DVTKTKYKET LYSGISKSFG MEPAPLTEMQ IYMKKKMFDP 850
KTSKVNKICD AVLNVLLSNP EYKKKYLQTI ITAYASQNPQ NLSAALKLIS 900
ELENSEEKDS CVTYLCFLQD VNVVYKSALS LYDVSLALLV AQKSQMDPRE 950
YLPFLQELQD NEPLRRKFLI DDYLGNYEKA LEHLSEIDKD GNVSEEVIDY 1000
VESHDLYKHG LALYRYDSEK QNVIYNIYAK HLSSNQMYTD AAVAYEMLGK 1050
LKEAMGAYQS AKRWREAMSI AVQKFPEEVE SVAEELISSL TFEHRYVDAA 1100
DIQLEYLDNV KEAVALYCKA YRYDIASLVA IKAKKDELLE EVVDPGLGEG 1150
FGIIAELLAD CKGQINSQLR RLRELRAKKE ENPYAFYGQE TEQADDVSVA 1200
PSETSTQESF FTRYTGKTGG TAKTGASRRT AKNKRREERK RARGKKGTIY 1250
EEEYLVQSVG RLIERLNQTK PDAVRVVEGL CRRNMREQAH QIQKNFVEVL 1300
DLLKANVKEI YSISEKDRER VNENGEVYYI PEIPVPEIHD FPKSHIVDF 1349
Length:1,349
Mass (Da):152,990
Last modified:November 1, 1996 - v1
Checksum:i7AEB1C0BA99282F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87841 Genomic DNA. Translation: BAA20120.1.
U19104 Genomic DNA. Translation: AAB67278.1.
BK006945 Genomic DNA. Translation: DAA09685.1.
PIRiS51471.
RefSeqiNP_013488.3. NM_001182273.3.

Genome annotation databases

EnsemblFungiiYLR384C; YLR384C; YLR384C.
GeneIDi851100.
KEGGisce:YLR384C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87841 Genomic DNA. Translation: BAA20120.1 .
U19104 Genomic DNA. Translation: AAB67278.1 .
BK006945 Genomic DNA. Translation: DAA09685.1 .
PIRi S51471.
RefSeqi NP_013488.3. NM_001182273.3.

3D structure databases

ProteinModelPortali Q06706.
SMRi Q06706. Positions 144-170, 356-382.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31643. 258 interactions.
DIPi DIP-2384N.
IntActi Q06706. 19 interactions.
MINTi MINT-567397.

Proteomic databases

MaxQBi Q06706.
PaxDbi Q06706.
PeptideAtlasi Q06706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR384C ; YLR384C ; YLR384C .
GeneIDi 851100.
KEGGi sce:YLR384C.

Organism-specific databases

CYGDi YLR384c.
SGDi S000004376. IKI3.

Phylogenomic databases

eggNOGi COG5290.
GeneTreei ENSGT00390000013344.
HOGENOMi HOG000188454.
KOi K11373.
OMAi TREVKNE.
OrthoDBi EOG7673K1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32450-MONOMER.

Miscellaneous databases

NextBioi 967788.
PROi Q06706.

Gene expression databases

Genevestigatori Q06706.

Family and domain databases

Gene3Di 2.130.10.10. 3 hits.
InterProi IPR006849. IKI3.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
PANTHERi PTHR12747. PTHR12747. 1 hit.
Pfami PF04762. IKI3. 1 hit.
[Graphical view ]
PIRSFi PIRSF017233. IKAP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
    Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
    Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
    Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
  5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
    Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
    EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
  6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
    Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
  7. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
    Krogan N.J., Greenblatt J.F.
    Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
  8. "Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
    Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
    Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ELP2.
  9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
    Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
  10. "Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
    Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
    J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
    Klassen R., Meinhardt F.
    Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
    Rahl P.B., Chen C.Z., Collins R.N.
    Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS REGULATION, INTERACTION WITH SEC2, SUBCELLULAR LOCATION.
  15. "An early step in wobble uridine tRNA modification requires the Elongator complex."
    Huang B., Johansson M.J.O., Bystroem A.S.
    RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRNA MODIFICATION.

Entry informationi

Entry nameiELP1_YEAST
AccessioniPrimary (citable) accession number: Q06706
Secondary accession number(s): D6VZ19, O00036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10500 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi