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Protein

Golgin IMH1

Gene

IMH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular transport between an endosomal compartment and the Golgi apparatus.2 Publications

GO - Biological processi

  • Golgi to plasma membrane protein transport Source: SGD
  • protein targeting to Golgi Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Stress response, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin IMH1
Alternative name(s):
Integrins and myosins homology protein 1
Gene namesi
Name:IMH1
Synonyms:SYS3
Ordered Locus Names:YLR309C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR309C.
SGDiS000004300. IMH1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi870 – 8701Y → A: Impairs interaction with ARL1 and Golgi localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Golgin IMH1PRO_0000240370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081PhosphoserineCombined sources
Modified residuei660 – 6601PhosphoserineCombined sources
Modified residuei827 – 8271PhosphoserineCombined sources
Modified residuei830 – 8301PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06704.

PTM databases

iPTMnetiQ06704.

Expressioni

Inductioni

By cold.1 Publication

Interactioni

Subunit structurei

Forms oligomers and is present in high-molecular-mass complexes. Interacts with ARL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARL1P381163EBI-33343,EBI-2869

Protein-protein interaction databases

BioGridi31574. 50 interactions.
DIPiDIP-5583N.
IntActiQ06704. 4 interactions.
MINTiMINT-565482.

Structurei

3D structure databases

ProteinModelPortaliQ06704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini861 – 90949GRIPPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili101 – 280180Sequence analysisAdd
BLAST
Coiled coili312 – 735424Sequence analysisAdd
BLAST
Coiled coili766 – 81449Sequence analysisAdd
BLAST

Domaini

The GRIP domain may serve as a Golgi targeting domain through its interaction with the ARL1 Golgi protein.2 Publications

Sequence similaritiesi

Contains 1 GRIP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000113062.
InParanoidiQ06704.
OMAiTESHEAL.
OrthoDBiEOG71P2KS.

Family and domain databases

InterProiIPR000237. GRIP.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKQLSQIGK NLTDELAKGL ADDMSPTPSE QQIEDDKSGL PKEIQAKLRK
60 70 80 90 100
FEKYEQKYPL LLSAYKNEKL KSEKLEAVEK ILAENTPISN IDDAVDTLPA
110 120 130 140 150
FFQDLNNKNN LLNDEIKRLT KQNSEIPESA SSETLKDKEE EFLKKEQNYK
160 170 180 190 200
NDIDDLKKKM EALNIELDTV QKEKNDTVSG LREKIVALEN ILKEEREAKK
210 220 230 240 250
QKEEVSISEL KEELAIKNHS LEDSRMKITE LEQNLSSKST IMEEKSSELA
260 270 280 290 300
ELNITLKEKE RKLSELEKKM KELPKAISHQ NVGNNNRRKK NRNKGKKNKG
310 320 330 340 350
GITTGDISEE ETVDNSINTE EYDKLKENLQ ELQEKYKDCE DWKQKYEDIE
360 370 380 390 400
AELKDAKELE NSQLEKSAKE LETLNTELID TKKSLKEKNS ELEEVRDMLR
410 420 430 440 450
TVGNELVDAK DEIKESSSKQ NEEVKTVKLE LDDLRHKNAT MIEAYEAKNT
460 470 480 490 500
ELRSKIELLS KKVEHLKNLC TEKEKEQTTS QNKVAKLNEE ISQLTYEKSN
510 520 530 540 550
ITKELTSLRT SYKQKEKTVS YLEEQVKQFS EQKDVAEKST EQLRKDHAKI
560 570 580 590 600
SNRLDLLKKE NETLHNDIAK NSNSYEEYLK ENGKLSERLN ILQEKYNTLQ
610 620 630 640 650
NVKSNSNEHI DSIKRQCEEL NVKLKESTKK ILSLEDELNE YANIVQDKTR
660 670 680 690 700
EANTLRRLVS DSQTDDSSKQ KELENKLAYL TDEKNKLEAE LDLQTSRKAT
710 720 730 740 750
ELQEWKHTVT ELKSEIHALK LREEGLKSEV DALKHVNNDI KRKTQATSDD
760 770 780 790 800
SDQLEQITSN LKLSLSKADE KNFELQSANE KLLNLNNELN KKFDRLLKNY
810 820 830 840 850
RSLSSQLNAL KERQYSDKSG RVSRSGSIGT LANANIDSSP ANNSNPTKLE
860 870 880 890 900
KIRSSSSLEL DSEKNEKIAY IKNVLLGFLE HKEQRNQLLP VISMLLQLDS
910
TDEKRLVMSL K
Length:911
Mass (Da):105,225
Last modified:November 1, 1996 - v1
Checksum:i090BD9AA5702C01D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17247 Genomic DNA. Translation: AAB67359.1.
BK006945 Genomic DNA. Translation: DAA09619.1.
PIRiS51441.
RefSeqiNP_013412.1. NM_001182197.2.

Genome annotation databases

EnsemblFungiiYLR309C; YLR309C; YLR309C.
GeneIDi851018.
KEGGisce:YLR309C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17247 Genomic DNA. Translation: AAB67359.1.
BK006945 Genomic DNA. Translation: DAA09619.1.
PIRiS51441.
RefSeqiNP_013412.1. NM_001182197.2.

3D structure databases

ProteinModelPortaliQ06704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31574. 50 interactions.
DIPiDIP-5583N.
IntActiQ06704. 4 interactions.
MINTiMINT-565482.

PTM databases

iPTMnetiQ06704.

Proteomic databases

MaxQBiQ06704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR309C; YLR309C; YLR309C.
GeneIDi851018.
KEGGisce:YLR309C.

Organism-specific databases

EuPathDBiFungiDB:YLR309C.
SGDiS000004300. IMH1.

Phylogenomic databases

HOGENOMiHOG000113062.
InParanoidiQ06704.
OMAiTESHEAL.
OrthoDBiEOG71P2KS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32395-MONOMER.

Miscellaneous databases

NextBioi967586.
PROiQ06704.

Family and domain databases

InterProiIPR000237. GRIP.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis."
    Li B., Warner J.R.
    J. Biol. Chem. 271:16813-16819(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structural and functional analysis of a novel coiled-coil protein involved in Ypt6 GTPase-regulated protein transport in yeast."
    Tsukada M., Will E., Gallwitz D.
    Mol. Biol. Cell 10:63-75(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  5. "The GRIP domain - a novel Golgi-targeting domain found in several coiled-coil proteins."
    Munro S., Nichols B.J.
    Curr. Biol. 9:377-380(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, SUBCELLULAR LOCATION.
  6. "A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins."
    Kjer-Nielsen L., Teasdale R.D., van Vliet C., Gleeson P.A.
    Curr. Biol. 9:385-388(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  7. "Gene expression analysis of cold and freeze stress in Baker's yeast."
    Rodriguez-Vargas S., Estruch F., Randez-Gil F.
    Appl. Environ. Microbiol. 68:3024-3030(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3."
    Setty S.R.G., Shin M.E., Yoshino A., Marks M.S., Burd C.G.
    Curr. Biol. 13:401-404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARL1, SUBCELLULAR LOCATION.
  9. "The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus."
    Panic B., Whyte J.R.C., Munro S.
    Curr. Biol. 13:405-410(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARL1, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-870.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p."
    Behnia R., Panic B., Whyte J.R.C., Munro S.
    Nat. Cell Biol. 6:405-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-308 AND SER-660, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-308; SER-827 AND THR-830, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIMH1_YEAST
AccessioniPrimary (citable) accession number: Q06704
Secondary accession number(s): D6VYV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.