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Reviewed, UniProtKB/Swiss-Prot Q06700 (GCDA_ACIFE)

Last modified May 5, 2009. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glutaconyl-CoA decarboxylase subunit alpha
    EC=4.1.1.70
Alternative name(s):
    Carboxyltransferase
Gene names
Name: gcdA
OrganismAcidaminococcus fermentans
Taxonomic identifier905 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Veillonellaceae › Acidaminococcus

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Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Decarboxylase subunit of the primary sodium pump glutaconyl-CoA decarboxylase (GCD).

Catalytic activity

4-carboxybut-2-enoyl-CoA = but-2-enoyl-CoA + CO2.

Pathway

Amino-acid degradation; L-glutamate degradation via hydroxyglutarate pathway; crotonoyl-CoA from L-glutamate: step 5/5.

Subunit structure

Heterooctamer consisting of two alpha, two beta, two gamma and two delta subunits.

Sequence similarities

Contains 1 carboxyltransferase domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Glutaconyl-CoA decarboxylase subunit alpha
PRO_0000087438

Regions

Domain35 – 568534Carboxyltransferase

Secondary structure

1 ................................................................................................... 587
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q06700-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BF1C0D2F2DCA86DA

FASTA58764,347
        10         20         30         40         50         60 
MGFYSMPRYF QNMPQVGKPL KKADAANEEQ LKKIEEEIHQ LIKEAQEAGK ADADVNKRGE 

        70         80         90        100        110        120 
LTALQRIEKL VEPGSWRPLN TLFNPQGNKN GSVAIVKGLG RVNGKWCVVV ASDNKKLAGA 

       130        140        150        160        170        180 
WVPGQAECLL RASDTAKTLH VPLVYVLNCS GVKFDEQEKV YPNRRGGGTP FFRNAELNQL 

       190        200        210        220        230        240 
GIPVIVGIYG TNPAGGGYHS ISPTVIIAHE KANMAVGGAG IMGGMNPKGH VDLEYANEIA 

       250        260        270        280        290        300 
DMVDRTGKTE PPGAVDIHYT ETGFMREVYA SEEGVLEGIK KYVGMLPKYD PEFFRVDDPK 

       310        320        330        340        350        360 
APAFPADDLY SMVPLNDKRA YDIYNVIARL FDNSELHEYK KGYGPEMVTG LAKVNGLLVG 

       370        380        390        400        410        420 
VVANVQGLLM NYPEYKAAGS VGIGGKLYRQ GLVKMNEFVT LCARDRLPIV WIQDTTGIDV 

       430        440        450        460        470        480 
GNDAEKAELL GLGQSLIYSI QTSHIPQFEI TLRKGTAAAH YVLGGPQGND TNAFSIGTAA 

       490        500        510        520        530        540 
TEIAVMNGET AATAMYSRRL AKDRKAGKDL QPTIDKMNNL IQAFYTKSRP KVCAELGLVD 

       550        560        570        580 
EIVDMNKIRG YVEAFTEAAY QNPESICPFH QMILPRAIRE FETFVKK

« Hide

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References

[1]"Cloning, sequencing and expression of the gene encoding the carboxytransferase subunit of the biotin-dependent Na+ pump glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in Escherichia coli."
Bendrat K., Buckel W.
Eur. J. Biochem. 211:697-702(1993) [PubMed: 8382157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 25085 / VR4 / DSM 20731 / CIP 106432.
[2]"Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli."
Bendrat K., Mueller U., Klees A.-G., Buckel W.
FEBS Lett. 329:329-331(1993) [PubMed: 8365476] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-587.
Strain: ATCC 25085 / VR4 / DSM 20731 / CIP 106432.
[3]"Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans."
Mueller U., Buckel W.
Eur. J. Biochem. 230:698-704(1995) [PubMed: 7607244] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-587.
Strain: ATCC 25085 / VR4 / DSM 20731 / CIP 106432.
[4]"Crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme A decarboxylase."
Wendt K.S., Schall I., Huber R., Buckel W., Jacob U.
EMBO J. 22:3493-3502(2003) [PubMed: 12853465] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

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Cross-references

Sequence databases

X69435 Genomic DNA. Translation: CAA49210.1.
X59645 Genomic DNA. Translation: CAA42195.1.
PIRS29787.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1PIXX-ray2.20A/B1-587[»]
ModBaseSearch...

Protein family/group databases

TCDB3.B.1.1.3. Na+-transporting carboxylic acid decarboxylase (NaT-DC) family.

Enzyme and pathway databases

BioCycMetaCyc:MON-1054.
BRENDA4.1.1.70. 74180.

Family and domain databases

InterProIPR000022. Carboxyl_trans.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
PROSITEPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGCDA_ACIFE
AccessionPrimary (citable) accession number: Q06700
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 5, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents