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Q06700 (GCDA_ACIFV) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaconyl-CoA decarboxylase subunit alpha

EC=4.1.1.70
Alternative name(s):
Carboxyltransferase
Gene names
Name:gcdA
Ordered Locus Names:Acfer_1817
OrganismAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4) [Complete proteome] [HAMAP]
Taxonomic identifier591001 [NCBI]
Taxonomic lineageBacteriaFirmicutesNegativicutesSelenomonadalesAcidaminococcaceaeAcidaminococcus

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Decarboxylase subunit of the primary sodium pump glutaconyl-CoA decarboxylase (GCD).

Catalytic activity

4-carboxybut-2-enoyl-CoA = but-2-enoyl-CoA + CO2.

Pathway

Amino-acid degradation; L-glutamate degradation via hydroxyglutarate pathway; crotonoyl-CoA from L-glutamate: step 5/5.

Subunit structure

Heterooctamer consisting of two alpha, two beta, two gamma and two delta subunits.

Sequence similarities

Contains 1 carboxyltransferase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Glutaconyl-CoA decarboxylase subunit alpha
PRO_0000087438

Regions

Domain35 – 568534Carboxyltransferase

Secondary structure

................................................................................................... 587
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q06700 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BF1C0D2F2DCA86DA

FASTA58764,347
        10         20         30         40         50         60 
MGFYSMPRYF QNMPQVGKPL KKADAANEEQ LKKIEEEIHQ LIKEAQEAGK ADADVNKRGE 

        70         80         90        100        110        120 
LTALQRIEKL VEPGSWRPLN TLFNPQGNKN GSVAIVKGLG RVNGKWCVVV ASDNKKLAGA 

       130        140        150        160        170        180 
WVPGQAECLL RASDTAKTLH VPLVYVLNCS GVKFDEQEKV YPNRRGGGTP FFRNAELNQL 

       190        200        210        220        230        240 
GIPVIVGIYG TNPAGGGYHS ISPTVIIAHE KANMAVGGAG IMGGMNPKGH VDLEYANEIA 

       250        260        270        280        290        300 
DMVDRTGKTE PPGAVDIHYT ETGFMREVYA SEEGVLEGIK KYVGMLPKYD PEFFRVDDPK 

       310        320        330        340        350        360 
APAFPADDLY SMVPLNDKRA YDIYNVIARL FDNSELHEYK KGYGPEMVTG LAKVNGLLVG 

       370        380        390        400        410        420 
VVANVQGLLM NYPEYKAAGS VGIGGKLYRQ GLVKMNEFVT LCARDRLPIV WIQDTTGIDV 

       430        440        450        460        470        480 
GNDAEKAELL GLGQSLIYSI QTSHIPQFEI TLRKGTAAAH YVLGGPQGND TNAFSIGTAA 

       490        500        510        520        530        540 
TEIAVMNGET AATAMYSRRL AKDRKAGKDL QPTIDKMNNL IQAFYTKSRP KVCAELGLVD 

       550        560        570        580 
EIVDMNKIRG YVEAFTEAAY QNPESICPFH QMILPRAIRE FETFVKK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and expression of the gene encoding the carboxytransferase subunit of the biotin-dependent Na+ pump glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in Escherichia coli."
Bendrat K., Buckel W.
Eur. J. Biochem. 211:697-702(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Complete genome sequence of Acidaminococcus fermentans type strain (VR4)."
Chang Y.J., Pukall R., Saunders E., Lapidus A., Copeland A., Nolan M., Glavina Del Rio T., Lucas S., Chen F., Tice H., Cheng J.F., Han C., Detter J.C., Bruce D., Goodwin L., Pitluck S., Mikhailova N., Liolios K. expand/collapse author list , Pati A., Ivanova N., Mavromatis K., Chen A., Palaniappan K., Land M., Hauser L., Jeffries C.D., Brettin T., Rohde M., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.
Stand. Genomic Sci. 3:1-14(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25085 / DSM 20731 / VR4.
[3]"Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli."
Bendrat K., Mueller U., Klees A.-G., Buckel W.
FEBS Lett. 329:329-331(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-587.
[4]"Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans."
Mueller U., Buckel W.
Eur. J. Biochem. 230:698-704(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-587.
[5]"Crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme A decarboxylase."
Wendt K.S., Schall I., Huber R., Buckel W., Jacob U.
EMBO J. 22:3493-3502(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69435 Genomic DNA. Translation: CAA49210.1.
CP001859 Genomic DNA. Translation: ADB48171.1.
X59645 Genomic DNA. Translation: CAA42195.1.
PIRS29787.
RefSeqYP_003399486.1. NC_013740.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PIXX-ray2.20A/B1-587[»]
ProteinModelPortalQ06700.
SMRQ06700. Positions 2-587.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB3.B.1.1.3. the na(+)-transporting carboxylic acid decarboxylase (nat-dc) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADB48171; ADB48171; Acfer_1817.
GeneID8738320.
KEGGafn:Acfer_1817.
PATRIC31910225. VBIAciFer109666_1808.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000064887.
KOK01615.
OMAGGMNPKG.

Enzyme and pathway databases

BioCycAFER591001:GHUL-1890-MONOMER.
MetaCyc:MONOMER-1054.
SABIO-RKQ06700.
UniPathwayUPA00533; UER00688.

Family and domain databases

Gene3D3.90.226.10. 2 hits.
InterProIPR000022. Carboxyl_trans.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
SUPFAMSSF52096. SSF52096. 2 hits.
PROSITEPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ06700.

Entry information

Entry nameGCDA_ACIFV
AccessionPrimary (citable) accession number: Q06700
Secondary accession number(s): D2RM69
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways