Putative ATP-dependent RNA helicase YLR419W - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Putative ATP-dependent RNA helicase YLR419W
EC=3.6.4.13

Gene names

Ordered Locus Names:

YLR419W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable ATP-binding RNA helicase By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 RWD domain. Contains 1 UBA domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1435

1435

Putative ATP-dependent RNA helicase YLR419W

PRO_0000247257

Regions

Domain

365 – 406

42

UBA

Domain

430 – 531

102

RWD

Domain

614 – 782

169

Helicase ATP-binding

Domain

845 – 1020

176

Helicase C-terminal

Nucleotide binding

627 – 634

8

ATP By similarity

Motif

729 – 732

4

DEAH box

Amino acid modifications

Modified residue

13

1

Phosphothreonine Ref.5

Modified residue

816

1

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q06698 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0F91A40BB8C7772B
Show »

FASTA

1,435

163,045

        10         20         30         40         50         60 
MAKKTKNNSK SSTPVNDVPT TAGKKKAKGK KGQEPEPEDD KRAKQQSNRA KVTSTASWTG 

        70         80         90        100        110        120 
KLPHTILHET CQKRKWNKVE YDMKKIGDKG FIAIAVLSFT DPKTKETLTA RMNDPTYDKA 

       130        140        150        160        170        180 
SGKGLVIPQE TPIEARHMAS TIALYRIAYN TNLHMMLPPN HRKTWYALDD FRKDNLKTDE 

       190        200        210        220        230        240 
KRINKLFDLD PFKTMVEDRK LKAQREKEQV AQNNQAQKEQ VARTILSSHG GISSSGKDRQ 

       250        260        270        280        290        300 
ERKVASHKNS HNPSLVRFPK KVWENSIFVD LDESSRQLIE TSLKEKIDWQ AKKISHKNET 

       310        320        330        340        350        360 
IAENREDLKA KLLTLQFRPK HVEEAMLYKD PLSFLLFNLP EDDLPPFFHK KKGDTKNKVE 

       370        380        390        400        410        420 
ITNLPLSTRM IVERLTEIGV SSDEALLALQ QNDMNENEAA GFLTREILPT LNSNTNEPVS 

       430        440        450        460        470        480 
ETESIECWNQ ELESLESIYE GCVMDAKEDS HYTLNLIEKL KIKLKVYRTK NYPASLPGIV 

       490        500        510        520        530        540 
VSTFDKNYKL PDYIKKQILT RLLHYLQEGN LIGDMLVYHI YEWLKENISK IIDNPGPLIP 

       550        560        570        580        590        600 
DSDSKGAINK RNISNGKRSI NNSSSRKFTK TTISEDTLSV LREEYTKRIK SSEYKSMQLV 

       610        620        630        640        650        660 
REQLPAWKKQ KVIIDIINKN EVVLITGETG SGKSTQVVQF ILDFLQKEKG DFGKTKIVCT 

       670        680        690        700        710        720 
QPRRISAIGL AERVSDERCV TCGEEVGYVI RGVNKTKAST RIKFMTTGVL VRLLQNARTM 

       730        740        750        760        770        780 
LENTIVVIDE VHERSIDTDL IVTLMKNLLH RVRGMKIVLM SATVNVDLFK KFFPGLATCH 

       790        800        810        820        830        840 
IEGRTFPITD YFLEDILSDL DFKIKREKAL SYDDDSVDER NNDDQYLKPR ADSKFFTSGQ 

       850        860        870        880        890        900 
INYDLLCQVV EYVHKRLKAA NDNGSIIVFL PGVGEINKCC NLLANKSNEA DFMVLPLHSA 

       910        920        930        940        950        960 
LTPEDQKRVF KKYHGKRKVV VSTNIAETSI TIDDCVATID TGRAKSMFYN PKDNTTKLIE 

       970        980        990       1000       1010       1020 
SFISKAEVKQ RRGRAGRVRE GLSYKLFSKN LYENDMISMP IPEIKRIPLE SLYLSVKAMG 

      1030       1040       1050       1060       1070       1080 
IKDVKAFLST ALDAPPLPAL QKAERILTTI GLVDESDKSL TQLGQFISLM PVMDSKHGKL 

      1090       1100       1110       1120       1130       1140 
LIYGILFGCT DISVLLVSIL GIGVLPFIGG FENREKIKKL LCKYESRGDL FAVLEIVRDY 

      1150       1160       1170       1180       1190       1200 
FKIKDSSIKR KYLRDNLLSY NKINEIKSST AQYYSILKDV GFLPMDYKVG SISDLNRNER 

      1210       1220       1230       1240       1250       1260 
NFDILRAILT GAFYPHIARV QLPDVKYLST SSGAVEKDPE AKMIKYWIRS EEYQDKLEEY 

      1270       1280       1290       1300       1310       1320 
KTKISQETQK VDLEDLPLPA TRAFIHPSSV LFSTNSVNLE DAKLLSEVDG PISRQSKIPT 

      1330       1340       1350       1360       1370       1380 
VVKYPFVLFT TSQVTNKLYL RDLTPTTTLS LLLFGGAISY DIGGTIHSPG IVVDNWLPIR 

      1390       1400       1410       1420       1430 
TWCKNGVLIK ELRTQLDEAI RKKLESPDYA KKSQIDNSGA DKTLKIVEKI IASEQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-13 AND SER-816, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20162 Genomic DNA. Translation: AAB67492.1. BK006945 Genomic DNA. Translation: DAA09721.1.
PIR	S59384.
RefSeq	NP_013523.3. NM_001182307.3.
3D structure databases
ProteinModelPortal	Q06698.
SMR	Q06698. Positions 585-1219.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06698. 2 interactions.
MINT	MINT-2492948.
STRING	4932.YLR419W.
Proteomic databases
PaxDb	Q06698.
PeptideAtlas	Q06698.
PRIDE	Q06698.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR419W; YLR419W; YLR419W.
GeneID	851137.
KEGG	sce:YLR419W. sce:YLR421C.
Organism-specific databases
CYGD	YLR419w.
SGD	S000004411. YLR419W.
Phylogenomic databases
eggNOG	COG1643.
GeneTree	ENSGT00680000101018.
HOGENOM	HOG000167651.
KO	K06691.
OMA	GWARIGV.
OrthoDB	EOG4Z65WS.
Gene expression databases
Genevestigator	Q06698.
GermOnline	YLR419W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR006575. RWD-domain. IPR015940. UBA/transl_elong_EF1B_N_euk. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. PF05773. RWD. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. SM00591. RWD. 1 hit. SM00165. UBA. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50908. RWD. 1 hit. PS50030. UBA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967890.

Entry information

Entry name

YL419_YEAST

Accession

Primary (citable) accession number: Q06698
Secondary accession number(s): D6VZ55

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents