Cell division control protein 73 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q06697 (CDC73_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell division control protein 73

Alternative name(s):
RNA polymerase-associated protein CDC73

Gene names

Name:	CDC73
Ordered Locus Names:	YLR418C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. Ref.3 Ref.7 Ref.8
Subunit structure	Component of the PAF1 complex which consists of at least CDC73, CTR9, LEO1, PAF1 and RTF1. Interacts with FACT subunits POB3 and SPT16. Ref.3 Ref.4 Ref.6
Subcellular location	Nucleus › nucleoplasm Ref.3 Ref.7.
Sequence similarities	Belongs to the CDC73 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of DNA recombination Inferred from mutant phenotype. Source: SGD positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Inferred from mutant phenotype. Source: SGD positive regulation of transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex Inferred from mutant phenotype. Source: SGD regulation of histone H2B conserved C-terminal lysine ubiquitination Inferred from direct assay. Source: SGD transcription elongation from RNA polymerase I promoter Inferred from mutant phenotype. Source: SGD
Cellular component	Cdc73/Paf1 complex Inferred from physical interaction Ref.3. Source: SGD transcriptionally active chromatin Inferred from direct assay. Source: SGD
Molecular function	RNA polymerase II core binding Inferred from physical interaction Ref.5 Ref.3. Source: SGD TFIIF-class binding transcription factor activity Inferred from mutant phenotype Ref.3. Source: SGD chromatin binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAF1	P38351	6	EBI-29913,EBI-12855
SLT2	Q00772	2	EBI-29913,EBI-17372

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 393

393

Cell division control protein 73

PRO_0000238580

Amino acid modifications

Modified residue

114

Phosphoserine Ref.9

Modified residue

143

Phosphoserine Ref.9

Modified residue

149

Phosphothreonine Ref.9

Modified residue

150

Phosphoserine Ref.9

Modified residue

191

Phosphotyrosine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

Q06697 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9580B53EEFB75345
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FASTA

393

44,456

        10         20         30         40         50         60 
MANSLDRLRE HLKNGDKLVL KNNEGQSTDD ITKATMVETL SSDGSTQDSF PLNEETEIEI 

        70         80         90        100        110        120 
DGSLVQLRII VHCWMNKDSS AADYLADCQN KQLTNVSFLQ RTDLINWLSG NTESSQYLKA 

       130        140        150        160        170        180 
PGQKGETSDK VDIENKTLAG ELSTVKSTTS ASLENDSEVS DPVVVETMKH ERILVDHNSA 

       190        200        210        220        230        240 
LRGAKPINFG YLIKDAELKL VQSIKGSLRG SKLPPGHKGA HGRISKTNGS SGGPRKDPII 

       250        260        270        280        290        300 
LIPSAASSIL TVANIKQFLL ESKYVNPRNL PSVPNGLVNI EKNFERISRP IRFIIVDNTR 

       310        320        330        340        350        360 
MFTKPEYWDR VVAIFTTGHT WQFNNYQWNS PQELFQRCKG YYFHFAGDSV PQHVQQWNVE 

       370        380        390 
KVELDKNKRF KDVEVVRYFW HSLEKELISR GYR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Cdc73p and Paf1p are found in a novel RNA polymerase II-containing complex distinct from the Srbp-containing holoenzyme." Shi X., Chang M., Wolf A.J., Chang C.-H., Frazer-Abel A.A., Wade P.A., Burton Z.F., Jaehning J.A. Mol. Cell. Biol. 17:1160-1169(1997) [PubMed: 9032243] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]	"A role for Ctr9p and Paf1p in the regulation of G1 cyclin expression in yeast." Koch C., Wollmann P., Dahl M., Lottspeich F. Nucleic Acids Res. 27:2126-2134(1999) [PubMed: 10219085] [Abstract] Cited for: SUBUNIT.
[5]	"Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex." Mueller C.L., Jaehning J.A. Mol. Cell. Biol. 22:1971-1980(2002) [PubMed: 11884586] [Abstract] Cited for: IDENTIFICATION IN THE PAF1 COMPLEX.
[6]	"RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach." Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F. Mol. Cell. Biol. 22:6979-6992(2002) [PubMed: 12242279] [Abstract] Cited for: INTERACTION WITH POB3 AND SPT16.
[7]	"Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization." Porter S.E., Penheiter K.L., Jaehning J.A. Eukaryot. Cell 4:209-220(2005) [PubMed: 15643076] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"A requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation." Sheldon K.E., Mauger D.M., Arndt K.M. Mol. Cell 20:225-236(2005) [PubMed: 16246725] [Abstract] Cited for: FUNCTION.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-143; THR-149; SER-150 AND TYR-191, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20162 Genomic DNA. Translation: AAB67500.1. BK006945 Genomic DNA. Translation: DAA09720.1.
PIR	S59383.
RefSeq	NP_013522.1. NM_001182306.1.
3D structure databases
ProteinModelPortal	Q06697.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1148N.
IntAct	Q06697. 19 interactions.
MINT	MINT-545984.
STRING	Q06697.
Proteomic databases
PeptideAtlas	Q06697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR418C; YLR418C; YLR418C.
GeneID	851136.
KEGG	sce:YLR418C.
NMPDR	fig\|4932.3.peg.4552.
Organism-specific databases
CYGD	YLR418c.
SGD	S000004410. CDC73.
Phylogenomic databases
eggNOG	fuNOG04767.
GeneTree	EFGT00050000001495.
HOGENOM	HBG395398.
OMA	TRFILVD.
OrthoDB	EOG4T1MWK.
Gene expression databases
ArrayExpress	Q06697.
Genevestigator	Q06697.
GermOnline	YLR418C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007852. RNA_pol_access_fac_Cdc73. [Graphical view]
KO	K15175.
PANTHER	PTHR12466. Cdc73. 1 hit.
Pfam	PF05179. CDC73. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967887.

Entry information

Entry name

CDC73_YEAST

Accession

Primary (citable) accession number: Q06697
Secondary accession number(s): D6VZ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents