Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein-sorting-associated protein 36

Gene

VPS36

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex, which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Involved in the trafficking of the plasma membrane ATPase. Its ability to bind ubiquitin plays a central role in endosomal sorting of ubiquitinated cargo proteins by the ESCRT complexes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri114 – 15138RanBP2-type 1; degenerateAdd
BLAST
Zinc fingeri177 – 20529RanBP2-type 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

  • CVT pathway Source: SGD
  • macroautophagy Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein retention in Golgi apparatus Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32478-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ESCRT-II complex subunit VPS36
Gene namesi
Name:VPS36
Synonyms:GRD12, VAC3, VPL11
Ordered Locus Names:YLR417W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR417W.
SGDiS000004409. VPS36.

Subcellular locationi

GO - Cellular componenti

  • ESCRT II complex Source: SGD
  • late endosome membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi187 – 1882TF → GS: Abolishes ubiquitin-binding and vacuole sorting of ubiquitinated proteins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Vacuolar protein-sorting-associated protein 36PRO_0000215227Add
BLAST

Proteomic databases

MaxQBiQ06696.

Interactioni

Subunit structurei

Component of the endosomal sorting required for transport complex II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex. Binds ubiquitin.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNF8Q124835EBI-36540,EBI-30277
UbcP629912EBI-36540,EBI-413074From a different organism.
VPS25P471423EBI-36540,EBI-25595
VPS28Q027676EBI-36540,EBI-20387

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi31675. 80 interactions.
DIPiDIP-1744N.
IntActiQ06696. 8 interactions.
MINTiMINT-401127.

Structurei

Secondary structure

1
566
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 3412Combined sources
Beta strandi43 – 6018Combined sources
Helixi62 – 654Combined sources
Beta strandi67 – 704Combined sources
Helixi71 – 733Combined sources
Beta strandi74 – 807Combined sources
Beta strandi82 – 876Combined sources
Beta strandi89 – 968Combined sources
Beta strandi116 – 1194Combined sources
Turni121 – 1233Combined sources
Beta strandi126 – 1316Combined sources
Turni144 – 1463Combined sources
Helixi152 – 1554Combined sources
Helixi156 – 1583Combined sources
Beta strandi256 – 2638Combined sources
Helixi266 – 28116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60B396-564[»]
1W7PX-ray3.60D1-566[»]
2CAYX-ray1.90A/B1-289[»]
2J9UX-ray2.00B/D110-171[»]
ProteinModelPortaliQ06696.
SMRiQ06696. Positions 1-103, 115-161, 251-281, 396-566.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06696.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 9690GLUE N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini255 – 28834GLUE C-terminalPROSITE-ProRule annotationAdd
BLAST

Domaini

The second RanBP2-type zinc-finger is functional and binds ubiquitin.1 Publication

Sequence similaritiesi

Belongs to the VPS36 family.Curated
Contains 1 GLUE C-terminal domain.PROSITE-ProRule annotation
Contains 1 GLUE N-terminal domain.PROSITE-ProRule annotation
Contains 2 RanBP2-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri114 – 15138RanBP2-type 1; degenerateAdd
BLAST
Zinc fingeri177 – 20529RanBP2-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000067728.
InParanoidiQ06696.
KOiK12190.
OMAiKCTFINH.
OrthoDBiEOG7FV406.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR021648. GLUE_dom.
IPR011993. PH_dom-like.
IPR031558. Vps36-NZF-N.
IPR011991. WHTH_DNA-bd_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF04157. EAP30. 1 hit.
PF16988. Vps36-NZF-N. 1 hit.
PF11605. Vps36_ESCRT-II. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS51495. GLUE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYWHYVETT SSGQPLLREG EKDIFIDQSV GLYHGKSKIL QRQRGRIFLT
60 70 80 90 100
SQRIIYIDDA KPTQNSLGLE LDDLAYVNYS SGFLTRSPRL ILFFKDPSSK
110 120 130 140 150
DELGKSAETA SADVVSTWVC PICMVSNETQ GEFTKDTLPT PICINCGVPA
160 170 180 190 200
DYELTKSSIN CSNAIDPNAN PQNQFGVNSE NICPACTFAN HPQIGNCEIC
210 220 230 240 250
GHRLPNASKV RSKLNRLNFH DSRVHIELEK NSLARNKSSH SALSSSSSTG
260 270 280 290 300
SSTEFVQLSF RKSDGVLFSQ ATERALENIL TEKNKHIFNQ NVVSVNGVDM
310 320 330 340 350
RKGASSHEYN NEVPFIETKL SRIGISSLEK SRENQLLNND ILFNNALTDL
360 370 380 390 400
NKLMSLATSI ERLYKNSNIT MKTKTLNLQD ESTVNEPKTR RPLLILDREK
410 420 430 440 450
FLNKELFLDE IAREIYEFTL SEFKDLNSDT NYMIITLVDL YAMYNKSMRI
460 470 480 490 500
GTGLISPMEM REACERFEHL GLNELKLVKV NKRILCVTSE KFDVVKEKLV
510 520 530 540 550
DLIGDNPGSD LLRLTQILSS NNSKSNWTLG ILMEVLQNCV DEGDLLIDKQ
560
LSGIYYYKNS YWPSHI
Length:566
Mass (Da):64,018
Last modified:November 1, 1996 - v1
Checksum:i60DDD4EA3A620409
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20162 Genomic DNA. Translation: AAB67493.1.
BK006945 Genomic DNA. Translation: DAA09719.1.
PIRiS59382.
RefSeqiNP_013521.3. NM_001182305.3.

Genome annotation databases

EnsemblFungiiYLR417W; YLR417W; YLR417W.
GeneIDi851135.
KEGGisce:YLR417W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20162 Genomic DNA. Translation: AAB67493.1.
BK006945 Genomic DNA. Translation: DAA09719.1.
PIRiS59382.
RefSeqiNP_013521.3. NM_001182305.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60B396-564[»]
1W7PX-ray3.60D1-566[»]
2CAYX-ray1.90A/B1-289[»]
2J9UX-ray2.00B/D110-171[»]
ProteinModelPortaliQ06696.
SMRiQ06696. Positions 1-103, 115-161, 251-281, 396-566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31675. 80 interactions.
DIPiDIP-1744N.
IntActiQ06696. 8 interactions.
MINTiMINT-401127.

Proteomic databases

MaxQBiQ06696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR417W; YLR417W; YLR417W.
GeneIDi851135.
KEGGisce:YLR417W.

Organism-specific databases

EuPathDBiFungiDB:YLR417W.
SGDiS000004409. VPS36.

Phylogenomic databases

HOGENOMiHOG000067728.
InParanoidiQ06696.
KOiK12190.
OMAiKCTFINH.
OrthoDBiEOG7FV406.

Enzyme and pathway databases

BioCyciYEAST:G3O-32478-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06696.
PROiQ06696.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR021648. GLUE_dom.
IPR011993. PH_dom-like.
IPR031558. Vps36-NZF-N.
IPR011991. WHTH_DNA-bd_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF04157. EAP30. 1 hit.
PF16988. Vps36-NZF-N. 1 hit.
PF11605. Vps36_ESCRT-II. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS51495. GLUE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body."
    Babst M., Katzmann D.J., Snyder W.B., Wendland B., Emr S.D.
    Dev. Cell 3:283-289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: DOMAIN, MUTAGENESIS OF 187-THR-PHE-188.
  6. "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes."
    Teo H., Perisic O., Gonzalez B., Williams R.L.
    Dev. Cell 7:559-569(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH VPS25 AND SNF8.
  7. "Structure of the ESCRT-II endosomal trafficking complex."
    Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H.
    Nature 431:221-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH VPS25 AND SNF8.

Entry informationi

Entry nameiVPS36_YEAST
AccessioniPrimary (citable) accession number: Q06696
Secondary accession number(s): D6VZ53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.