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Q06685

- VIP1_YEAST

UniProt

Q06685 - VIP1_YEAST

Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase

Gene

VIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 By similarity. Required for maintaining cellular integrity, normal growth and interactions with the ARP complex. Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability. Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth.By similarity3 Publications

    Catalytic activityi

    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.1 Publication
    ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
    ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei278 – 2781ATPBy similarity
    Binding sitei351 – 3511ATPBy similarity
    Binding sitei358 – 3581ATPBy similarity
    Binding sitei377 – 3771ATPBy similarity
    Binding sitei414 – 4141SubstrateBy similarity
    Binding sitei428 – 4281SubstrateBy similarity
    Binding sitei430 – 4301ATPBy similarity
    Binding sitei475 – 4751ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi402 – 4054ATPBy similarity
    Nucleotide bindingi412 – 4143ATPBy similarity
    Nucleotide bindingi487 – 4893ATPBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB-EC
    4. inositol heptakisphosphate kinase activity Source: SGD
    5. inositol hexakisphosphate 1-kinase activity Source: SGD
    6. inositol hexakisphosphate 3-kinase activity Source: SGD
    7. inositol hexakisphosphate 4-kinase activity Source: SGD
    8. inositol hexakisphosphate 5-kinase activity Source: UniProtKB-EC
    9. inositol hexakisphosphate 6-kinase activity Source: SGD

    GO - Biological processi

    1. inositol phosphate biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32472-MONOMER.
    YEAST:MONOMER3O-224.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase (EC:2.7.4.21, EC:2.7.4.24)
    Alternative name(s):
    InsP6 and PP-IP5 kinase
    Gene namesi
    Name:VIP1
    Ordered Locus Names:YLR410W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR410w.
    SGDiS000004402. VIP1.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi487 – 4871D → A: Abolishes enzyme activity. 1 Publication
    Mutagenesisi548 – 5481H → A: Does not affect enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11461146Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinasePRO_0000270924Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei31 – 311Phosphoserine2 Publications
    Modified residuei54 – 541Phosphoserine1 Publication
    Modified residuei77 – 771Phosphoserine1 Publication
    Modified residuei895 – 8951Phosphoserine1 Publication
    Modified residuei1107 – 11071Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ06685.
    PaxDbiQ06685.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06685.

    Interactioni

    Protein-protein interaction databases

    BioGridi31667. 198 interactions.
    IntActiQ06685. 31 interactions.
    MINTiMINT-2784338.
    STRINGi4932.YLR410W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06685.
    SMRiQ06685. Positions 190-508.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 1982Substrate bindingBy similarity
    Regioni377 – 3782Substrate bindingBy similarity
    Regioni492 – 4954Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG245915.
    GeneTreeiENSGT00390000009048.
    HOGENOMiHOG000177917.
    OMAiYPINILA.
    OrthoDBiEOG7673KF.

    Family and domain databases

    Gene3Di3.30.470.20. 1 hit.
    3.40.50.1240. 4 hits.
    InterProiIPR013651. ATP-grasp_RimK-type.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    Q06685-1 [UniParc]FASTAAdd to Basket

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    MSGIKKEPIE SDEVPQQETK NNLPSAPSEM SPLFLNKNTQ KAMQSIAPIL     50
    EGFSPKTSAS ENMSLKLPPP GIQDDHSEEN LTVHDTLQRT ISTALGNGNN 100
    TNTVTTSGLK KADSESKSEA DPEGLSNSNI VNDADNINSI SKTGSPHLPQ 150
    GTMDAEQTNM GTNSVPTSSA SSRKSSTSHP KPRLPKVGKI GVCAMDAKVL 200
    SKPMRHILNR LIEHGEFETV IFGDKVILDE RIENWPTCDF LISFFSSGFP 250
    LDKAIKYVKL RKPFIINDLI MQKILWDRRL CLQVLEAYNV PTPPRLEISR 300
    DGGPRANEEL RAKLREHGVE VKPVEEPEWK MVDDDTLEVD GKTMTKPFVE 350
    KPVDGEDHNI YIYYHSKNGG GGRRLFRKVG NKSSEFDPTL VHPRTEGSYI 400
    YEQFMDTDNF EDVKAYTIGE NFCHAETRKS PVVDGIVRRN THGKEVRYIT 450
    ELSDEEKTIA GKVSKAFSQM ICGFDLLRVS GKSYVIDVNG FSFVKDNKAY 500
    YDSCANILRS TFIEAKKKMD MEKKNLPIIR EEKEQKWVFK GLAIIIRHAD 550
    RTPKQKFKHS FTSPIFISLL KGHKEEVVIR NVNDLKIVLQ ALRIALDEKA 600
    GNPAKIKVLA NALEKKLNFP GTKIQLKPVL NKENEVEKVQ FILKWGGEPT 650
    HSAKYQATEL GEQMRQDFDL LNKSILQNIK IFSSSERRVL HTAQYWTRAL 700
    FGADELGSDE ISIRKDLLDD SNAAKDLMDK VKKKLKPLLR EGKEAPPQFA 750
    WPSKMPEPYL VIKRVVELMN YHKKIMDNNF AKKDVNSMQT RWCTSEDPSL 800
    FKERWDKLFK EFNNAEKVDP SKISELYDTM KYDALHNRQF LENIFDPGLP 850
    NEAIADELGS HSLVDRYPIN VLAKNNFKII DSHSMNNSGK NSSNSVGSLG 900
    WVLESGKTST ARNPKSSSQF DEPRFMQLRE LYKLAKVLFD FICPKEYGIS 950
    DAEKLDIGLL TSLPLAKQIL NDIGDMKNRE TPACVAYFTK ESHIYTLLNI 1000
    IYESGIPMRI ARNALPELDY LSQITFELYE STDASGQKSH SIRLKMSPGC 1050
    HTQDPLDVQL DDRHYISCIP KISLTKHLDM DYVQQKLRNK FTRVIMPPKF 1100
    TPVNITSPNL SFQKRKTRRK SVSVEKLKRP ASSGSSSSTS VNKTLD 1146
    Length:1,146
    Mass (Da):129,755
    Last modified:November 1, 1996 - v1
    Checksum:iD69E44EAD16490F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20162 Genomic DNA. Translation: AAB67497.1.
    BK006945 Genomic DNA. Translation: DAA09710.1.
    PIRiS59376.
    RefSeqiNP_013514.1. NM_001182298.1.

    Genome annotation databases

    EnsemblFungiiYLR410W; YLR410W; YLR410W.
    GeneIDi851126.
    KEGGisce:YLR410W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20162 Genomic DNA. Translation: AAB67497.1 .
    BK006945 Genomic DNA. Translation: DAA09710.1 .
    PIRi S59376.
    RefSeqi NP_013514.1. NM_001182298.1.

    3D structure databases

    ProteinModelPortali Q06685.
    SMRi Q06685. Positions 190-508.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31667. 198 interactions.
    IntActi Q06685. 31 interactions.
    MINTi MINT-2784338.
    STRINGi 4932.YLR410W.

    Proteomic databases

    MaxQBi Q06685.
    PaxDbi Q06685.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR410W ; YLR410W ; YLR410W .
    GeneIDi 851126.
    KEGGi sce:YLR410W.

    Organism-specific databases

    CYGDi YLR410w.
    SGDi S000004402. VIP1.

    Phylogenomic databases

    eggNOGi NOG245915.
    GeneTreei ENSGT00390000009048.
    HOGENOMi HOG000177917.
    OMAi YPINILA.
    OrthoDBi EOG7673KF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32472-MONOMER.
    YEAST:MONOMER3O-224.

    Miscellaneous databases

    NextBioi 967860.
    PROi Q06685.

    Gene expression databases

    Genevestigatori Q06685.

    Family and domain databases

    Gene3Di 3.30.470.20. 1 hit.
    3.40.50.1240. 4 hits.
    InterProi IPR013651. ATP-grasp_RimK-type.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    PF08443. RimK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin."
      Feoktistova A., McCollum D., Ohi R., Gould K.L.
      Genetics 152:895-908(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A conserved family of enzymes that phosphorylate inositol hexakisphosphate."
      Mulugu S., Bai W., Fridy P.C., Bastidas R.J., Otto J.C., Dollins D.E., Haystead T.A., Ribeiro A.A., York J.D.
      Science 316:106-109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-487 AND HIS-548.
    9. "Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates."
      Lee Y.-S., Mulugu S., York J.D., O'Shea E.K.
      Science 316:109-112(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-895 AND SER-1107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVIP1_YEAST
    AccessioniPrimary (citable) accession number: Q06685
    Secondary accession number(s): D6VZ44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8810 molecules/cell in log phase SD medium.1 Publication

    Caution

    Although related to histidine acid phosphatase proteins, it lacks the conserved active sites, suggesting that it has no phosphatase activity.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3