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Q06685

- VIP1_YEAST

UniProt

Q06685 - VIP1_YEAST

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Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase

Gene

VIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 (By similarity). Required for maintaining cellular integrity, normal growth and interactions with the ARP complex. Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability. Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth.By similarity3 Publications

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei278 – 2781ATPBy similarity
Binding sitei351 – 3511ATPBy similarity
Binding sitei358 – 3581ATPBy similarity
Binding sitei377 – 3771ATPBy similarity
Binding sitei414 – 4141SubstrateBy similarity
Binding sitei428 – 4281SubstrateBy similarity
Binding sitei430 – 4301ATPBy similarity
Binding sitei475 – 4751ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi402 – 4054ATPBy similarity
Nucleotide bindingi412 – 4143ATPBy similarity
Nucleotide bindingi487 – 4893ATPBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB-EC
  4. inositol heptakisphosphate kinase activity Source: SGD
  5. inositol hexakisphosphate 1-kinase activity Source: SGD
  6. inositol hexakisphosphate 3-kinase activity Source: SGD
  7. inositol hexakisphosphate 4-kinase activity Source: SGD
  8. inositol hexakisphosphate 5-kinase activity Source: UniProtKB-EC
  9. inositol hexakisphosphate 6-kinase activity Source: SGD

GO - Biological processi

  1. inositol phosphate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32472-MONOMER.
YEAST:MONOMER3O-224.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase (EC:2.7.4.21, EC:2.7.4.24)
Alternative name(s):
InsP6 and PP-IP5 kinase
Gene namesi
Name:VIP1
Ordered Locus Names:YLR410W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR410w.
SGDiS000004402. VIP1.

Subcellular locationi

Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi487 – 4871D → A: Abolishes enzyme activity. 1 Publication
Mutagenesisi548 – 5481H → A: Does not affect enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11461146Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinasePRO_0000270924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311Phosphoserine2 Publications
Modified residuei54 – 541Phosphoserine1 Publication
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei895 – 8951Phosphoserine1 Publication
Modified residuei1107 – 11071Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06685.
PaxDbiQ06685.

Expressioni

Gene expression databases

GenevestigatoriQ06685.

Interactioni

Protein-protein interaction databases

BioGridi31667. 199 interactions.
IntActiQ06685. 31 interactions.
MINTiMINT-2784338.
STRINGi4932.YLR410W.

Structurei

3D structure databases

ProteinModelPortaliQ06685.
SMRiQ06685. Positions 190-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 1982Substrate bindingBy similarity
Regioni377 – 3782Substrate bindingBy similarity
Regioni492 – 4954Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG245915.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
InParanoidiQ06685.
OMAiYPINILA.
OrthoDBiEOG7673KF.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.1240. 4 hits.
InterProiIPR013651. ATP-grasp_RimK-type.
IPR013816. ATP_grasp_subdomain_2.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 4 hits.

Sequencei

Sequence statusi: Complete.

Q06685 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGIKKEPIE SDEVPQQETK NNLPSAPSEM SPLFLNKNTQ KAMQSIAPIL
60 70 80 90 100
EGFSPKTSAS ENMSLKLPPP GIQDDHSEEN LTVHDTLQRT ISTALGNGNN
110 120 130 140 150
TNTVTTSGLK KADSESKSEA DPEGLSNSNI VNDADNINSI SKTGSPHLPQ
160 170 180 190 200
GTMDAEQTNM GTNSVPTSSA SSRKSSTSHP KPRLPKVGKI GVCAMDAKVL
210 220 230 240 250
SKPMRHILNR LIEHGEFETV IFGDKVILDE RIENWPTCDF LISFFSSGFP
260 270 280 290 300
LDKAIKYVKL RKPFIINDLI MQKILWDRRL CLQVLEAYNV PTPPRLEISR
310 320 330 340 350
DGGPRANEEL RAKLREHGVE VKPVEEPEWK MVDDDTLEVD GKTMTKPFVE
360 370 380 390 400
KPVDGEDHNI YIYYHSKNGG GGRRLFRKVG NKSSEFDPTL VHPRTEGSYI
410 420 430 440 450
YEQFMDTDNF EDVKAYTIGE NFCHAETRKS PVVDGIVRRN THGKEVRYIT
460 470 480 490 500
ELSDEEKTIA GKVSKAFSQM ICGFDLLRVS GKSYVIDVNG FSFVKDNKAY
510 520 530 540 550
YDSCANILRS TFIEAKKKMD MEKKNLPIIR EEKEQKWVFK GLAIIIRHAD
560 570 580 590 600
RTPKQKFKHS FTSPIFISLL KGHKEEVVIR NVNDLKIVLQ ALRIALDEKA
610 620 630 640 650
GNPAKIKVLA NALEKKLNFP GTKIQLKPVL NKENEVEKVQ FILKWGGEPT
660 670 680 690 700
HSAKYQATEL GEQMRQDFDL LNKSILQNIK IFSSSERRVL HTAQYWTRAL
710 720 730 740 750
FGADELGSDE ISIRKDLLDD SNAAKDLMDK VKKKLKPLLR EGKEAPPQFA
760 770 780 790 800
WPSKMPEPYL VIKRVVELMN YHKKIMDNNF AKKDVNSMQT RWCTSEDPSL
810 820 830 840 850
FKERWDKLFK EFNNAEKVDP SKISELYDTM KYDALHNRQF LENIFDPGLP
860 870 880 890 900
NEAIADELGS HSLVDRYPIN VLAKNNFKII DSHSMNNSGK NSSNSVGSLG
910 920 930 940 950
WVLESGKTST ARNPKSSSQF DEPRFMQLRE LYKLAKVLFD FICPKEYGIS
960 970 980 990 1000
DAEKLDIGLL TSLPLAKQIL NDIGDMKNRE TPACVAYFTK ESHIYTLLNI
1010 1020 1030 1040 1050
IYESGIPMRI ARNALPELDY LSQITFELYE STDASGQKSH SIRLKMSPGC
1060 1070 1080 1090 1100
HTQDPLDVQL DDRHYISCIP KISLTKHLDM DYVQQKLRNK FTRVIMPPKF
1110 1120 1130 1140
TPVNITSPNL SFQKRKTRRK SVSVEKLKRP ASSGSSSSTS VNKTLD
Length:1,146
Mass (Da):129,755
Last modified:November 1, 1996 - v1
Checksum:iD69E44EAD16490F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20162 Genomic DNA. Translation: AAB67497.1.
BK006945 Genomic DNA. Translation: DAA09710.1.
PIRiS59376.
RefSeqiNP_013514.1. NM_001182298.1.

Genome annotation databases

EnsemblFungiiYLR410W; YLR410W; YLR410W.
GeneIDi851126.
KEGGisce:YLR410W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20162 Genomic DNA. Translation: AAB67497.1 .
BK006945 Genomic DNA. Translation: DAA09710.1 .
PIRi S59376.
RefSeqi NP_013514.1. NM_001182298.1.

3D structure databases

ProteinModelPortali Q06685.
SMRi Q06685. Positions 190-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31667. 199 interactions.
IntActi Q06685. 31 interactions.
MINTi MINT-2784338.
STRINGi 4932.YLR410W.

Proteomic databases

MaxQBi Q06685.
PaxDbi Q06685.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR410W ; YLR410W ; YLR410W .
GeneIDi 851126.
KEGGi sce:YLR410W.

Organism-specific databases

CYGDi YLR410w.
SGDi S000004402. VIP1.

Phylogenomic databases

eggNOGi NOG245915.
GeneTreei ENSGT00390000009048.
HOGENOMi HOG000177917.
InParanoidi Q06685.
OMAi YPINILA.
OrthoDBi EOG7673KF.

Enzyme and pathway databases

BioCyci YEAST:G3O-32472-MONOMER.
YEAST:MONOMER3O-224.

Miscellaneous databases

NextBioi 967860.
PROi Q06685.

Gene expression databases

Genevestigatori Q06685.

Family and domain databases

Gene3Di 3.30.470.20. 1 hit.
3.40.50.1240. 4 hits.
InterProi IPR013651. ATP-grasp_RimK-type.
IPR013816. ATP_grasp_subdomain_2.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
PF08443. RimK. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin."
    Feoktistova A., McCollum D., Ohi R., Gould K.L.
    Genetics 152:895-908(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A conserved family of enzymes that phosphorylate inositol hexakisphosphate."
    Mulugu S., Bai W., Fridy P.C., Bastidas R.J., Otto J.C., Dollins D.E., Haystead T.A., Ribeiro A.A., York J.D.
    Science 316:106-109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-487 AND HIS-548.
  9. "Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates."
    Lee Y.-S., Mulugu S., York J.D., O'Shea E.K.
    Science 316:109-112(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-895 AND SER-1107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVIP1_YEAST
AccessioniPrimary (citable) accession number: Q06685
Secondary accession number(s): D6VZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8810 molecules/cell in log phase SD medium.1 Publication

Caution

Although related to histidine acid phosphatase proteins, it lacks the conserved active sites, suggesting that it has no phosphatase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3