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Q06678

- RM35_YEAST

UniProt

Q06678 - RM35_YEAST

Protein

54S ribosomal protein L35, mitochondrial

Gene

MRPL35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29879-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L35, mitochondrial
    Alternative name(s):
    YmL35
    Gene namesi
    Name:MRPL35
    Ordered Locus Names:YDR322W
    ORF Names:D9798.5
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR322w.
    SGDiS000002730. MRPL35.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2929MitochondrionSequence AnalysisAdd
    BLAST
    Chaini30 – 36733854S ribosomal protein L35, mitochondrialPRO_0000023284Add
    BLAST

    Proteomic databases

    MaxQBiQ06678.
    PaxDbiQ06678.
    PeptideAtlasiQ06678.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06678.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

    Protein-protein interaction databases

    BioGridi32379. 45 interactions.
    DIPiDIP-6832N.
    IntActiQ06678. 12 interactions.
    MINTiMINT-640904.
    STRINGi4932.YDR322W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW4electron microscopy3.2011-367[»]
    ProteinModelPortaliQ06678.
    SMRiQ06678. Positions 156-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG323873.
    HOGENOMiHOG000001057.
    KOiK17439.
    OMAiFPFSTGV.
    OrthoDBiEOG7ZWDCC.

    Family and domain databases

    Gene3Di3.90.280.10. 1 hit.
    InterProiIPR008914. PtdEtn-bd_prot_PEBP.
    [Graphical view]
    PfamiPF01161. PBP. 1 hit.
    [Graphical view]
    SUPFAMiSSF49777. SSF49777. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06678-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRRSIHTTK ILQKPNATSH IWSDFTTRPS SLSIQSSKVK NYLFQKKASL    50
    DPPSISRRSN RIKYSPPEHI DEIFRMSYDF LEQRSSKFYE LANKTKNPLK 100
    KDALLIKAEI NNPEVQYNFQ FNNKLNNVKD IIDYDVPVYR HLGKQHWESY 150
    GQMLLMQRLE TLAAIPDTLP TLVPRAEVNI KFPFSTGVNK WIEPGEFLSS 200
    NVTSMRPIFK IQEYELVNVE KQLYTVLIVN PDVPDLSNDS FKTALCYGLV 250
    NINLTYNDNL IDPRKFHSSN IIADYLPPVP EKNAGKQRFV VWVFRQPLIE 300
    DKQGPNMLEI DRKELSRDDF DIRQFTKKYN LTAIGAHIWR SEWDAKVAAV 350
    REKYGLPPGR VFSRVRR 367
    Length:367
    Mass (Da):42,825
    Last modified:November 1, 1996 - v1
    Checksum:iC6A2C973121FF555
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1131P → D AA sequence (PubMed:9151978)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32517 Genomic DNA. Translation: AAB64758.1.
    BK006938 Genomic DNA. Translation: DAA12164.1.
    PIRiS59788.
    RefSeqiNP_010608.1. NM_001180630.1.

    Genome annotation databases

    EnsemblFungiiYDR322W; YDR322W; YDR322W.
    GeneIDi851921.
    KEGGisce:YDR322W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32517 Genomic DNA. Translation: AAB64758.1 .
    BK006938 Genomic DNA. Translation: DAA12164.1 .
    PIRi S59788.
    RefSeqi NP_010608.1. NM_001180630.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW4 electron microscopy 3.20 1 1-367 [» ]
    ProteinModelPortali Q06678.
    SMRi Q06678. Positions 156-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32379. 45 interactions.
    DIPi DIP-6832N.
    IntActi Q06678. 12 interactions.
    MINTi MINT-640904.
    STRINGi 4932.YDR322W.

    Proteomic databases

    MaxQBi Q06678.
    PaxDbi Q06678.
    PeptideAtlasi Q06678.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR322W ; YDR322W ; YDR322W .
    GeneIDi 851921.
    KEGGi sce:YDR322W.

    Organism-specific databases

    CYGDi YDR322w.
    SGDi S000002730. MRPL35.

    Phylogenomic databases

    eggNOGi NOG323873.
    HOGENOMi HOG000001057.
    KOi K17439.
    OMAi FPFSTGV.
    OrthoDBi EOG7ZWDCC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29879-MONOMER.

    Miscellaneous databases

    NextBioi 969965.

    Gene expression databases

    Genevestigatori Q06678.

    Family and domain databases

    Gene3Di 3.90.280.10. 1 hit.
    InterProi IPR008914. PtdEtn-bd_prot_PEBP.
    [Graphical view ]
    Pfami PF01161. PBP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49777. SSF49777. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism."
      Dziembowski A., Piwowarski J., Hoser R., Minczuk M., Dmochowska A., Siep M., van der Spek H., Grivell L.A., Stepien P.P.
      J. Biol. Chem. 278:1603-1611(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 64-84; 130-140; 159-175 AND 289-295.
    4. "Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae."
      Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K.
      Eur. J. Biochem. 245:449-456(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 108-116, SUBUNIT.
      Strain: 07173.
    5. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
      Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
      Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiRM35_YEAST
    AccessioniPrimary (citable) accession number: Q06678
    Secondary accession number(s): D6VSV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3