Reviewed, UniProtKB/Swiss-Prot Q06650 (BLAC_STRCE)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

bla

Organism

Streptomyces cellulosae

Taxonomic identifier

1968 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes benzylpenicillin and cloxacillin (at 10% of the rate of benzylpenicillin).
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	NADP
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Tat-type signal Potential
	Chain	37 – 311	275	Beta-lactamase		PRO_0000017015
Regions
	Region	252 – 254	3	Substrate binding By similarity
Sites
	Active site	86	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06650-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F3578EBEEA92A3FB	FASTA	311	33,137
	10 20 30 40 50 60 MRKPTSSLTR RSVLGAGLGL GGALALGSTT ASAASAGTTP SENPAAVRRL RALEREHQAR 70 80 90 100 110 120 IGVFALNLAT GASLLHRAHE LFPMCSVFKT LAAAAVLRDL DHDGSQLARV IRYTEADVTK 130 140 150 160 170 180 SGHAPVTKDH IDTGMTIRDL CDATIRYSDN CAANLLLREL GGPTAVTRFC RSLGDPVTRL 190 200 210 220 230 240 DRWEPELNSG EPDRRTDTTS PYAIARTYQR LVLGNALNRP DRALLTDWLL RNTTTLTTFR 250 260 270 280 290 300 TGLPKGWTVA DKSGGGDTYG TRNEAAIAWT PDGAPVLLTA LTHKPSLPTA PGDTPLIIKL 310 ATVLSEAVAP A

References

[1]	"Sequence of a gene encoding beta-lactamase from Streptomyces cellulosae." Ogawara H. Gene 124:111-114(1993) [PubMed: 7916705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KCC S0127.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12653 Genomic DNA. Translation: BAA02176.1.
PIR	JN0520.
3D structure databases
SMR	Q06650. Positions 47-308.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 290663.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_STRCE

Accession

Primary (citable) accession number: Q06650

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)