Q06648 (GIC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase-interacting component 2

Gene names

Name:	GIC2
Ordered Locus Names:	YDR309C
ORF Names:	D9740.18

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for cell size and shape control, bud site selection, bud emergence, actin cytoskeletal organization, mitotic spindle orientation/positioning, and mating projection formation in response to mating pheromone.
Subunit structure	Interacts with GTP-bound CDC42.
Subcellular location	Bud neck By similarity. Bud tip By similarity. Cytoplasm › cell cortex By similarity. Cytoplasm › cytoskeleton By similarity.
Miscellaneous	Present with 1130 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the BORG/CEP family. Contains 1 CRIB domain.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cytoplasm Cytoskeleton
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD axial cellular bud site selection Traceable author statement. Source: SGD regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of exit from mitosis Inferred from physical interaction. Source: SGD
Cellular component	actin cap Traceable author statement. Source: SGD cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud tip Inferred from direct assay Ref.5. Source: SGD incipient cellular bud site Inferred from direct assay Ref.5. Source: SGD mating projection tip Inferred from direct assay Ref.5. Source: SGD plasma membrane Inferred from genetic interaction. Source: SGD
Molecular function	Rho GTPase activator activity Inferred from direct assay Ref.5. Source: SGD Rho GTPase binding Inferred from physical interaction Ref.5. Source: SGD phosphatidylinositol-4,5-bisphosphate binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC11	P32458	2	EBI-7585,EBI-4178
CDC12	P32468	5	EBI-7585,EBI-4182
CDC3	P32457	3	EBI-7585,EBI-4429

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	GTPase-interacting component 2		PRO_0000212660
Regions
	Domain	134 – 147	14	CRIB
	Compositional bias	74 – 102	29	Ser-rich
Amino acid modifications
	Modified residue	99	1	Phosphoserine Ref.9
	Modified residue	254	1	Phosphoserine Ref.7
	Modified residue	258	1	Phosphoserine Ref.7
	Modified residue	337	1	Phosphoserine Ref.9 Ref.10
	Modified residue	345	1	Phosphoserine Ref.8
	Modified residue	367	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q06648 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D1BE002BCD48EB03	FASTA	383	42,852
	10 20 30 40 50 60 MTSASITNTG NETMNLPQMR SIWLDEDEEA EKLYGLQAQQ FMGSDDEENL GITFINSDKP 70 80 90 100 110 120 VLSNKKNIEL PPLSPNSHPS CHHRRSNSNS AKSKESSSSS SSANKTNHKK VFLKLNLLKK 130 140 150 160 170 180 KLLGAQPDIR GKGISTPFDF QHISHADTRN GFQDEQLQEP SSLSTEIKDD YTSSSSKRDS 190 200 210 220 230 240 KSLNKAFVTE RIPANRESKL ISRSHENKTS RLSVARSISV TSSNYSKNTQ GNNHSINGRV 250 260 270 280 290 300 VSTSTMATSI FEYSPNASPK QFKNKSHALG HRYTNSTDSS ESSLDFLKNY NFPTLLEDKP 310 320 330 340 350 360 ILDFLPRSQR SSAYRSLLET PNSNKDSAKA FFPSRQSPLP KRRNSIATPS PQSKFSYSDS 370 380 PVNHRKSFDD VLYSFNQLEP LQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae." Chen G.C., Kim Y.J., Chan C.S. Genes Dev. 11:2958-2971(1997) [PubMed: 9367979] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast." Brown J.L., Jaquenoud M., Gulli M.P., Chant J., Peter M. Genes Dev. 11:2972-2982(1997) [PubMed: 9367980] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254; SER-258 AND SER-367, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-337, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28374 Genomic DNA. Translation: AAB64745.1. AY557791 Genomic DNA. Translation: AAS56117.1. BK006938 Genomic DNA. Translation: DAA12148.1.
PIR	S61195.
RefSeq	NP_010595.1. NM_001180617.1.
3D structure databases
ProteinModelPortal	Q06648.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2277N.
IntAct	Q06648. 23 interactions.
MINT	MINT-558890.
STRING	Q06648.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR309C; YDR309C; YDR309C.
GeneID	851904.
KEGG	sce:YDR309C.
NMPDR	fig|4932.3.peg.1357.
Organism-specific databases
CYGD	YDR309c.
SGD	S000002717. GIC2.
Phylogenomic databases
eggNOG	fuNOG11722.
GeneTree	EFGT00050000008595.
HOGENOM	HBG203246.
OMA	QHISHAD.
OrthoDB	EOG40S3R8.
Gene expression databases
ArrayExpress	Q06648.
Genevestigator	Q06648.
GermOnline	YDR309C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000095. PAK_box_Rho-bd. [Graphical view]
Pfam	PF00786. PBD. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969915.

Entry information

Entry name

GIC2_YEAST

Accession

Primary (citable) accession number: Q06648
Secondary accession number(s): D6VST8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program