SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q06625

- GDE_YEAST

UniProt

Q06625 - GDE_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glycogen debranching enzyme

Gene
GDB1, YPR184W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.2 Publications

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.

Enzyme regulationi

Activity is inhibited by IGD1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei535 – 5351 By similarity
Active sitei538 – 5381 By similarity
Active sitei670 – 6701 By similarity

GO - Molecular functioni

  1. 4-alpha-glucanotransferase activity Source: SGD
  2. amylo-alpha-1,6-glucosidase activity Source: SGD

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-KW
  2. glycogen catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13683.
YEAST:YPR184W-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme
Alternative name(s):
Glycogen debrancher
Including the following 2 domains:
4-alpha-glucanotransferase (EC:2.4.1.25)
Alternative name(s):
Oligo-1,4-1,4-glucantransferase
Amylo-alpha-1,6-glucosidase (EC:3.2.1.33)
Short name:
Amylo-1,6-glucosidase
Alternative name(s):
Dextrin 6-alpha-D-glucosidase
Gene namesi
Name:GDB1
Ordered Locus Names:YPR184W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR184w.
SGDiS000006388. GDB1.

Subcellular locationi

Mitochondrion. Cytoplasm 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15361536Glycogen debranching enzymePRO_0000087452Add
BLAST

Proteomic databases

MaxQBiQ06625.
PaxDbiQ06625.
PeptideAtlasiQ06625.
PRIDEiQ06625.

Expressioni

Gene expression databases

GenevestigatoriQ06625.

Interactioni

Subunit structurei

Interacts with IGD1.1 Publication

Protein-protein interaction databases

BioGridi36356. 26 interactions.
DIPiDIP-2578N.
IntActiQ06625. 6 interactions.
MINTiMINT-532857.
STRINGi4932.YPR184W.

Structurei

3D structure databases

ProteinModelPortaliQ06625.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni? – 1536Amylo-1,6-glucosidase
Regioni1 – ?4-alpha-glucanotransferase

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3408.
GeneTreeiENSGT00390000012596.
HOGENOMiHOG000212981.
KOiK01196.
OMAiPCLRPLM.
OrthoDBiEOG74J9H3.

Family and domain databases

Gene3Di3.20.20.80. 3 hits.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10569. PTHR10569. 1 hit.
PfamiPF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 3 hits.
TIGRFAMsiTIGR01531. glyc_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06625-1 [UniParc]FASTAAdd to Basket

« Hide

MNRSLLLRLS DTGEPITSCS YGKGVLTLPP IPLPKDAPKD QPLYTVKLLV     50
SAGSPVARDG LVWTNCPPDH NTPFKRDKFY KKIIHSSFHE DDCIDLNVYA 100
PGSYCFYLSF RNDNEKLETT RKYYFVALPM LYINDQFLPL NSIALQSVVS 150
KWLGSDWEPI LSKIAAKNYN MVHFTPLQER GESNSPYSIY DQLQFDQEHF 200
KSPEDVKNLV EHIHRDLNML SLTDIVFNHT ANNSPWLVEH PEAGYNHITA 250
PHLISAIELD QELLNFSRNL KSWGYPTELK NIEDLFKIMD GIKVHVLGSL 300
KLWEYYAVNV QTALRDIKAH WNDESNESYS FPENIKDISS DFVKLASFVK 350
DNVTEPNFGT LGERNSNRIN VPKFIQLLKL INDGGSDDSE SSLATAQNIL 400
NEVNLPLYRE YDDDVSEILE QLFNRIKYLR LDDGGPKQGP VTVDVPLTEP 450
YFTRFKGKDG TDYALANNGW IWNGNPLVDF ASQNSRAYLR REVIVWGDCV 500
KLRYGKSPED SPYLWERMSK YIEMNAKIFD GFRIDNCHST PIHVGEYFLD 550
LARKYNPNLY VVAELFSGSE TLDCLFVERL GISSLIREAM QAWSEEELSR 600
LVHKHGGRPI GSYKFVPMDD FSYPADINLN EEHCFNDSND NSIRCVSEIM 650
IPKILTATPP HALFMDCTHD NETPFEKRTV EDTLPNAALV ALCSSAIGSV 700
YGYDEIFPHL LNLVTEKRHY DISTPTGSPS IGITKVKATL NSIRTSIGEK 750
AYDIEDSEMH VHHQGQYITF HRMDVKSGKG WYLIARMKFS DNDDPNETLP 800
PVVLNQSTCS LRFSYALERV GDEIPNDDKF IKGIPTKLKE LEGFDISYDD 850
SKKISTIKLP NEFPQGSIAI FETQQNGVDE SLDHFIRSGA LKATSSLTLE 900
SINSVLYRSE PEEYDVSAGE GGAYIIPNFG KPVYCGLQGW VSVLRKIVFY 950
NDLAHPLSAN LRNGHWALDY TISRLNYYSD EAGINEVQNW LRSRFDRVKK 1000
LPSYLVPSYF ALIIGILYGC CRLKAIQLMS RNIGKSTLFV QSLSMTSIQM 1050
VSRMKSTSIL PGENVPSMAA GLPHFSVNYM RCWGRDVFIS LRGMLLTTGR 1100
FDEAKAHILA FAKTLKHGLI PNLLDAGRNP RYNARDAAWF FLQAVQDYVY 1150
IVPDGEKILQ EQVTRRFPLD DTYIPVDDPR AFSYSSTLEE IIYEILSRHA 1200
KGIKFREANA GPNLDRVMTD KGFNVEIHVD WSTGLIHGGS QYNCGTWMDK 1250
MGESEKAGSV GIPGTPRDGA AIEINGLLKS ALRFVIELKN KGLFKFSDVE 1300
TQDGGRIDFT EWNQLLQDNF EKRYYVPEDP SQDADYDVSA KLGVNRRGIY 1350
RDLYKSGKPY EDYQLRPNFA IAMTVAPELF VPEHAIKAIT IADEVLRGPV 1400
GMRTLDPSDY NYRPYYNNGE DSDDFATSKG RNYHQGPEWV WLYGYFLRAF 1450
HHFHFKTSPR CQNAAKEKPS SYLYQQLYYR LKGHRKWIFE SVWAGLTELT 1500
NKDGEVCNDS SPTQAWSSAC LLDLFYDLWD AYEDDS 1536
Length:1,536
Mass (Da):174,972
Last modified:November 1, 1996 - v1
Checksum:i167270B2EE15EDF9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti368 – 3681R → K in BAA34996. 1 Publication
Sequence conflicti536 – 5361N → Y in BAA34996. 1 Publication
Sequence conflicti774 – 7741D → N in BAA34996. 1 Publication
Sequence conflicti1180 – 11801R → K in BAA34996. 1 Publication
Sequence conflicti1289 – 12891K → N in BAA34996. 1 Publication
Sequence conflicti1489 – 14891F → S in BAA34996. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018078 Genomic DNA. Translation: BAA34996.1.
U25842 Genomic DNA. Translation: AAB68117.1.
BK006949 Genomic DNA. Translation: DAA11600.1.
PIRiS59841.
RefSeqiNP_015510.1. NM_001184281.1.

Genome annotation databases

EnsemblFungiiYPR184W; YPR184W; YPR184W.
GeneIDi856314.
KEGGisce:YPR184W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB018078 Genomic DNA. Translation: BAA34996.1 .
U25842 Genomic DNA. Translation: AAB68117.1 .
BK006949 Genomic DNA. Translation: DAA11600.1 .
PIRi S59841.
RefSeqi NP_015510.1. NM_001184281.1.

3D structure databases

ProteinModelPortali Q06625.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36356. 26 interactions.
DIPi DIP-2578N.
IntActi Q06625. 6 interactions.
MINTi MINT-532857.
STRINGi 4932.YPR184W.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Proteomic databases

MaxQBi Q06625.
PaxDbi Q06625.
PeptideAtlasi Q06625.
PRIDEi Q06625.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR184W ; YPR184W ; YPR184W .
GeneIDi 856314.
KEGGi sce:YPR184W.

Organism-specific databases

CYGDi YPR184w.
SGDi S000006388. GDB1.

Phylogenomic databases

eggNOGi COG3408.
GeneTreei ENSGT00390000012596.
HOGENOMi HOG000212981.
KOi K01196.
OMAi PCLRPLM.
OrthoDBi EOG74J9H3.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13683.
YEAST:YPR184W-MONOMER.

Miscellaneous databases

NextBioi 981694.
PROi Q06625.

Gene expression databases

Genevestigatori Q06625.

Family and domain databases

Gene3Di 3.20.20.80. 3 hits.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR010401. AGL/Gdb1.
IPR029436. AGL_euk_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR006421. Glycogen_debranch_met.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10569. PTHR10569. 1 hit.
Pfami PF06202. GDE_C. 1 hit.
PF14699. hGDE_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 2 hits.
SSF51445. SSF51445. 3 hits.
TIGRFAMsi TIGR01531. glyc_debranch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "High expression of glycogen-debranching enzyme in Escherichia coli and its competent purification method."
    Nakayama A., Yamamoto K., Tabata S.
    Protein Expr. Purif. 19:298-303(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 208-216; 302-311; 351-358; 1296-1305 AND 1356-1365.
    Strain: ATCC 56960 / D-346.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae YPR184w gene encodes the glycogen debranching enzyme."
    Teste M.A., Enjalbert B., Parrou J.L., Francois J.M.
    FEMS Microbiol. Lett. 193:105-110(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme."
    Walkey C.J., Luo Z., Borchers C.H., Measday V., van Vuuren H.J.
    FEMS Yeast Res. 11:499-508(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IGD1, ENZYME REGULATION, FUNCTION.

Entry informationi

Entry nameiGDE_YEAST
AccessioniPrimary (citable) accession number: Q06625
Secondary accession number(s): D6W4I4, O93808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi