Reviewed, UniProtKB/Swiss-Prot Q06623 (HDA3_YEAST)

Names and origin

Protein names

Recommended name:
    HDA1 complex subunit 3
Alternative name(s):
    Histone deacetylase complex 1 subunit 3

Gene names

Name:	HDA3
Synonyms:	PLO1
Ordered Locus Names:	YPR179C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for activity of HDA1 histone deacetylase complex. The HDA1 histone deacetylase complex is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Ref.2 Ref.3
Subunit structure	Heterodimer with HDA2. Component of the HDA1 histone deacetylase complex composed of at least one HDA1 homodimer and one HDA2/HDA3 heterodimer. Interacts with HDA1 and HDA3. Ref.3
Subcellular location	Nucleus. Ref.4
Miscellaneous	Present with 1100 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the HDA2/3 family. HDA3 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chromatin regulator Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from mutant phenotype. Source: SGD gene silencing by RNA Inferred from mutant phenotype. Source: SGD histone deacetylation Ref.2 Ref.3 Inferred from direct assay. Source: SGD negative regulation of transcription from RNA polymerase II promoter Ref.3 Inferred from mutant phenotype. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	histone deacetylase complex Ref.2 Ref.3 Inferred from direct assay. Source: SGD
Molecular function	chromatin binding Inferred from direct assay. Source: SGD histone deacetylase activity Ref.2 Ref.3 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TUP1	P16649	1	EBI-38663,EBI-19654
YCL021W-A	Q96VH3	1	EBI-38663,EBI-2044941

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	HDA1 complex subunit 3		PRO_0000083932
Regions
	Coiled coil	482 – 632	151	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06623-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CC8F8F73D6B78E45	FASTA	655	75,463
	10 20 30 40 50 60 MDLLRILDTK PIPTIVDATT LGISGNTSGD YWLPTTMSLY QKELTDQIVS LHYSDILRYF 70 80 90 100 110 120 ETSHYKEDVI LESMKTMCLN GSLVATHPYL LIDHYMPKSL ITRDVPAHLA ENSGKFSVLR 130 140 150 160 170 180 DLINLVQEYE TETAIVCRPG RTMDLLEALL LGNKVHIKRY DGHSIKSKQK ANDFSCTVHL 190 200 210 220 230 240 FSSEGINFTK YPIKSKARFD MLICLDTTVD TSQKDIQYLL QYKRERKGLE RYAPIVRLVA 250 260 270 280 290 300 INSIDHCRLF FGKKFDKNSR EYLENVTAAM VILRDRLGTL PPDLRPIYSQ KLHYLVEWLE 310 320 330 340 350 360 NPTVPWPLPD IYPLKQYTSM DVERSLLTEV HFKKSDDQLE DAFSNCSKKR GRHGANKAAS 370 380 390 400 410 420 STVAGIEDNI TPSFYSTKRL KNDYYTNPLK QDMTQLTGIT TADNSSNVNY HLSSGIITHK 430 440 450 460 470 480 LIQSMGEVYM DICVQKQELD DYSCLDDLQN DHLKFFSNED EKIIKEYETV LRTNNENLNR 490 500 510 520 530 540 SHELEVENNL KFSQIETLEK DIETLKGSLM AQGETLSKLK DAFVKTDNVQ DEIEKEERVS 550 560 570 580 590 600 VSRDTEKKYM EQEIKRAVDA IRENEEETHK LNEKQNGLES ELKLKFEKSE ISTKELNEKI 610 620 630 640 650 GFLKKELKLE NDLNEELVGQ LSKTMDNLEN LTIPRVRTQN GNTKKKSRAK KPGNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"HDA1 and HDA3 are components of a yeast histone deacetylase (HDA) complex." Carmen A.A., Rundlett S.E., Grunstein M. J. Biol. Chem. 271:15837-15844(1996) [PubMed: 8663039] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE HDA1 HISTONE DEACETYLASE COMPLEX.
[3]	"HDA2 and HDA3 are related proteins that interact with and are essential for the activity of the yeast histone deacetylase HDA1." Wu J., Carmen A.A., Kobayashi R., Suka N., Grunstein M. Proc. Natl. Acad. Sci. U.S.A. 98:4391-4396(2001) [PubMed: 11287668] [Abstract] Cited for: FUNCTION, INTERACTION WITH HDA1 AND HDA3.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U25842 Genomic DNA. Translation: AAB68112.1.
PIR	S59836.
RefSeq	NP_015505.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4636N.
IntAct	Q06623. 13 interactions.
Proteomic databases
PeptideAtlas	Q06623.
Genome annotation databases
Ensembl	YPR179C. Saccharomyces cerevisiae. [Contig view]
GeneID	856309.
GenomeReviews	Gene locus YPR179C in contig U00094_GR.
KEGG	sce:YPR179C.
NMPDR	fig|4932.3.peg.6654.
Organism-specific databases
CYGD	YPR179c.
SGD	S000006383. HDA3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06623.
OMA	Q06623. YQKELTD.
Gene expression databases
ArrayExpress	Q06623.
GermOnline	YPR179C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	981679.

Entry information

Entry name

HDA3_YEAST

Accession

Primary (citable) accession number: Q06623

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)