Q06611 (PIP12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin PIP1-2
Short name=AtPIP1;2

Alternative name(s):
Plasma membrane intrinsic protein 1b
Short name=PIP1b
Transmembrane protein A
Short name=AthH2
Short name=TMP-A

Gene names

Name:	PIP1-2
Synonyms:	PIP1B, TMPA
Ordered Locus Names:	At2g45960
ORF Names:	F4I18.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across cell membrane. Essential for the water permeability of the plasma membrane and for the morphology of the root system. Its function is impaired by Hg2+. Inhibited by cytosolic acidosis which occurs during anoxia in roots. Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.8.
Tissue specificity	Widely expressed. Expressed in roots and above ground. Ref.9
Induction	By blue light and abscisic acid (ABA).
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q06611-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Aquaporin PIP1-2		PRO_0000064047
Regions
	Topological domain	1 – 54	54	Cytoplasmic Potential
	Transmembrane	55 – 75	21	Helical; Name=1; Potential
	Topological domain	76 – 81	6	Extracellular Potential
	Transmembrane	82 – 102	21	Helical; Name=2; Potential
	Topological domain	103 – 132	30	Cytoplasmic Potential
	Transmembrane	133 – 153	21	Helical; Name=3; Potential
	Topological domain	154 – 174	21	Extracellular Potential
	Transmembrane	175 – 195	21	Helical; Name=4; Potential
	Topological domain	196 – 208	13	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Helical; Name=5; Potential
	Topological domain	230 – 256	27	Extracellular Potential
	Transmembrane	257 – 277	21	Helical; Name=6; Potential
	Topological domain	278 – 286	9	Cytoplasmic Potential
	Motif	114 – 116	3	NPA 1
	Motif	235 – 237	3	NPA 2
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.11
Experimental info
	Sequence conflict	29	1	Missing in AAM61041. Ref.6
	Sequence conflict	40	1	F → L in CAB37860. Ref.2
	Sequence conflict	279	1	A → T in AAM61041. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 80F12775B6809013	FASTA	286	30,598
	10 20 30 40 50 60 MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPLF EPGELASWSF WRAGIAEFIA 70 80 90 100 110 120 TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 130 140 150 160 170 180 LFLARKLSLT RAVYYIVMQC LGAICGAGVV KGFQPKQYQA LGGGANTIAH GYTKGSGLGA 190 200 210 220 230 240 EIIGTFVLVY TVFSATDAKR NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 250 260 270 280 GAAIIFNKDN AWDDHWVFWV GPFIGAALAA LYHVIVIRAI PFKSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of an Arabidopsis thaliana turgor-responsive cDNA clone encoding TMP-A, a transmembrane protein containing the major intrinsic protein motif." Shagan T., Bar-Zvi D. Plant Physiol. 101:1397-1398(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Etiolated seedling.
[2]	"A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein." Kaldenhoff R., Koelling A., Richter G. Plant Mol. Biol. 23:1187-1198(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C24.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-286. Strain: cv. Columbia. Tissue: Seedling.
[8]	"Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency." Cutler S.R., Ehrhardt D.W., Griffitts J.S., Somerville C.R. Proc. Natl. Acad. Sci. U.S.A. 97:3718-3723(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[10]	"Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins." Tournaire-Roux C., Sutka M., Javot H., Gout E., Gerbeau P., Luu D.-T., Bligny R., Maurel C. Nature 425:393-397(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Methylation of aquaporins in plant plasma membrane." Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C. Biochem. J. 400:189-197(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68293 mRNA. Translation: CAA48356.1. Z17424 mRNA. Translation: CAB37860.1. AC004665 Genomic DNA. Translation: AAC28529.1. CP002685 Genomic DNA. Translation: AEC10622.1. AY063931 mRNA. Translation: AAL36287.1. AY048294 mRNA. Translation: AAK82556.1. AY091254 mRNA. Translation: AAM14193.1. AY084470 mRNA. Translation: AAM61041.1. Z25965 mRNA. Translation: CAA81120.1.
IPI	IPI00536778.
PIR	T02451.
RefSeq	NP_182120.1. NM_130159.3.
UniGene	At.20518. At.24183.
3D structure databases
ProteinModelPortal	Q06611.
SMR	Q06611. Positions 43-276.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06611. 8 interactions.
Proteomic databases
PaxDb	Q06611.
PRIDE	Q06611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G45960.1; AT2G45960.1; AT2G45960.
GeneID	819204.
KEGG	ath:AT2G45960.
Organism-specific databases
GeneFarm	4863. 182.
TAIR	At2g45960.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288286.
InParanoid	Q06611.
KO	K09872.
PhylomeDB	Q06611.
ProtClustDB	CLSN2682869.
Gene expression databases
ArrayExpress	Q06611.
Genevestigator	Q06611.
GermOnline	AT2G45960. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIP12_ARATH

Accession

Primary (citable) accession number: Q06611
Secondary accession number(s): Q8L5V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program