Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q06611

- PIP12_ARATH

UniProt

Q06611 - PIP12_ARATH

Protein

Aquaporin PIP1-2

Gene

PIP1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Water channel required to facilitate the transport of water across cell membrane. Essential for the water permeability of the plasma membrane and for the morphology of the root system. Its function is impaired by Hg2+. Inhibited by cytosolic acidosis which occurs during anoxia in roots.1 Publication

    GO - Molecular functioni

    1. transporter activity Source: InterPro

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_187715. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_187717. Erythrocytes take up oxygen and release carbon dioxide.
    REACT_206372. Regulation of water balance by renal Aquaporins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aquaporin PIP1-2
    Short name:
    AtPIP1;2
    Alternative name(s):
    Plasma membrane intrinsic protein 1b
    Short name:
    PIP1b
    Transmembrane protein A
    Short name:
    AthH2
    Short name:
    TMP-A
    Gene namesi
    Name:PIP1-2
    Synonyms:PIP1B, TMPA
    Ordered Locus Names:At2g45960
    ORF Names:F4I18.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G45960.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286Aquaporin PIP1-2PRO_0000064047Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei284 – 2841PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ06611.
    PRIDEiQ06611.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in roots and above ground.1 Publication

    Inductioni

    By blue light and abscisic acid (ABA).

    Gene expression databases

    ArrayExpressiQ06611.
    GenevestigatoriQ06611.

    Interactioni

    Protein-protein interaction databases

    BioGridi4540. 10 interactions.
    IntActiQ06611. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06611.
    SMRiQ06611. Positions 43-276.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5454CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini76 – 816ExtracellularSequence Analysis
    Topological domaini103 – 13230CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini154 – 17421ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini196 – 20813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini230 – 25627ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini278 – 2869CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei55 – 7521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei257 – 27721Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi114 – 1163NPA 1
    Motifi235 – 2373NPA 2

    Domaini

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0580.
    HOGENOMiHOG000288286.
    InParanoidiQ06611.
    KOiK09872.
    PhylomeDBiQ06611.

    Family and domain databases

    Gene3Di1.20.1080.10. 1 hit.
    InterProiIPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view]
    PANTHERiPTHR19139. PTHR19139. 1 hit.
    PfamiPF00230. MIP. 1 hit.
    [Graphical view]
    PRINTSiPR00783. MINTRINSICP.
    SUPFAMiSSF81338. SSF81338. 1 hit.
    TIGRFAMsiTIGR00861. MIP. 1 hit.
    PROSITEiPS00221. MIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q06611-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPLF EPGELASWSF    50
    WRAGIAEFIA TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA 100
    LVYCTAGISG GHINPAVTFG LFLARKLSLT RAVYYIVMQC LGAICGAGVV 150
    KGFQPKQYQA LGGGANTIAH GYTKGSGLGA EIIGTFVLVY TVFSATDAKR 200
    NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL GAAIIFNKDN 250
    AWDDHWVFWV GPFIGAALAA LYHVIVIRAI PFKSRS 286
    Length:286
    Mass (Da):30,598
    Last modified:November 1, 1995 - v1
    Checksum:i80F12775B6809013
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291Missing in AAM61041. 1 PublicationCurated
    Sequence conflicti40 – 401F → L in CAB37860. (PubMed:8292783)Curated
    Sequence conflicti279 – 2791A → T in AAM61041. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68293 mRNA. Translation: CAA48356.1.
    Z17424 mRNA. Translation: CAB37860.1.
    AC004665 Genomic DNA. Translation: AAC28529.1.
    CP002685 Genomic DNA. Translation: AEC10622.1.
    AY063931 mRNA. Translation: AAL36287.1.
    AY048294 mRNA. Translation: AAK82556.1.
    AY091254 mRNA. Translation: AAM14193.1.
    AY084470 mRNA. Translation: AAM61041.1.
    Z25965 mRNA. Translation: CAA81120.1.
    PIRiT02451.
    RefSeqiNP_182120.1. NM_130159.3. [Q06611-1]
    UniGeneiAt.20518.
    At.24183.

    Genome annotation databases

    EnsemblPlantsiAT2G45960.1; AT2G45960.1; AT2G45960. [Q06611-1]
    GeneIDi819204.
    KEGGiath:AT2G45960.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68293 mRNA. Translation: CAA48356.1 .
    Z17424 mRNA. Translation: CAB37860.1 .
    AC004665 Genomic DNA. Translation: AAC28529.1 .
    CP002685 Genomic DNA. Translation: AEC10622.1 .
    AY063931 mRNA. Translation: AAL36287.1 .
    AY048294 mRNA. Translation: AAK82556.1 .
    AY091254 mRNA. Translation: AAM14193.1 .
    AY084470 mRNA. Translation: AAM61041.1 .
    Z25965 mRNA. Translation: CAA81120.1 .
    PIRi T02451.
    RefSeqi NP_182120.1. NM_130159.3. [Q06611-1 ]
    UniGenei At.20518.
    At.24183.

    3D structure databases

    ProteinModelPortali Q06611.
    SMRi Q06611. Positions 43-276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4540. 10 interactions.
    IntActi Q06611. 9 interactions.

    Proteomic databases

    PaxDbi Q06611.
    PRIDEi Q06611.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G45960.1 ; AT2G45960.1 ; AT2G45960 . [Q06611-1 ]
    GeneIDi 819204.
    KEGGi ath:AT2G45960.

    Organism-specific databases

    GeneFarmi 4863. 182.
    TAIRi AT2G45960.

    Phylogenomic databases

    eggNOGi COG0580.
    HOGENOMi HOG000288286.
    InParanoidi Q06611.
    KOi K09872.
    PhylomeDBi Q06611.

    Enzyme and pathway databases

    Reactomei REACT_187715. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_187717. Erythrocytes take up oxygen and release carbon dioxide.
    REACT_206372. Regulation of water balance by renal Aquaporins.

    Gene expression databases

    ArrayExpressi Q06611.
    Genevestigatori Q06611.

    Family and domain databases

    Gene3Di 1.20.1080.10. 1 hit.
    InterProi IPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view ]
    PANTHERi PTHR19139. PTHR19139. 1 hit.
    Pfami PF00230. MIP. 1 hit.
    [Graphical view ]
    PRINTSi PR00783. MINTRINSICP.
    SUPFAMi SSF81338. SSF81338. 1 hit.
    TIGRFAMsi TIGR00861. MIP. 1 hit.
    PROSITEi PS00221. MIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of an Arabidopsis thaliana turgor-responsive cDNA clone encoding TMP-A, a transmembrane protein containing the major intrinsic protein motif."
      Shagan T., Bar-Zvi D.
      Plant Physiol. 101:1397-1398(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Etiolated seedling.
    2. "A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein."
      Kaldenhoff R., Koelling A., Richter G.
      Plant Mol. Biol. 23:1187-1198(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-286.
      Strain: cv. Columbia.
      Tissue: Seedling.
    8. "Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency."
      Cutler S.R., Ehrhardt D.W., Griffitts J.S., Somerville C.R.
      Proc. Natl. Acad. Sci. U.S.A. 97:3718-3723(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "From genome to function: the Arabidopsis aquaporins."
      Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.
      Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
    10. "Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins."
      Tournaire-Roux C., Sutka M., Javot H., Gout E., Gerbeau P., Luu D.-T., Bligny R., Maurel C.
      Nature 425:393-397(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPIP12_ARATH
    AccessioniPrimary (citable) accession number: Q06611
    Secondary accession number(s): Q8L5V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3