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Q06611 (PIP12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aquaporin PIP1-2

Short name=AtPIP1;2
Alternative name(s):
Plasma membrane intrinsic protein 1b
Short name=PIP1b
Transmembrane protein A
Short name=AthH2
Short name=TMP-A
Gene names
Name:PIP1-2
Synonyms:PIP1B, TMPA
Ordered Locus Names:At2g45960
ORF Names:F4I18.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Water channel required to facilitate the transport of water across cell membrane. Essential for the water permeability of the plasma membrane and for the morphology of the root system. Its function is impaired by Hg2+. Inhibited by cytosolic acidosis which occurs during anoxia in roots. Ref.10

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.8.

Tissue specificity

Widely expressed. Expressed in roots and above ground. Ref.9

Induction

By blue light and abscisic acid (ABA).

Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Transmembrane
Transmembrane helix
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q06611-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Aquaporin PIP1-2
PRO_0000064047

Regions

Topological domain1 – 5454Cytoplasmic Potential
Transmembrane55 – 7521Helical; Name=1; Potential
Topological domain76 – 816Extracellular Potential
Transmembrane82 – 10221Helical; Name=2; Potential
Topological domain103 – 13230Cytoplasmic Potential
Transmembrane133 – 15321Helical; Name=3; Potential
Topological domain154 – 17421Extracellular Potential
Transmembrane175 – 19521Helical; Name=4; Potential
Topological domain196 – 20813Cytoplasmic Potential
Transmembrane209 – 22921Helical; Name=5; Potential
Topological domain230 – 25627Extracellular Potential
Transmembrane257 – 27721Helical; Name=6; Potential
Topological domain278 – 2869Cytoplasmic Potential
Motif114 – 1163NPA 1
Motif235 – 2373NPA 2

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11
Modified residue2841Phosphoserine By similarity

Experimental info

Sequence conflict291Missing in AAM61041. Ref.6
Sequence conflict401F → L in CAB37860. Ref.2
Sequence conflict2791A → T in AAM61041. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 80F12775B6809013

FASTA28630,598
        10         20         30         40         50         60 
MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPLF EPGELASWSF WRAGIAEFIA 

        70         80         90        100        110        120 
TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 

       130        140        150        160        170        180 
LFLARKLSLT RAVYYIVMQC LGAICGAGVV KGFQPKQYQA LGGGANTIAH GYTKGSGLGA 

       190        200        210        220        230        240 
EIIGTFVLVY TVFSATDAKR NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 

       250        260        270        280 
GAAIIFNKDN AWDDHWVFWV GPFIGAALAA LYHVIVIRAI PFKSRS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of an Arabidopsis thaliana turgor-responsive cDNA clone encoding TMP-A, a transmembrane protein containing the major intrinsic protein motif."
Shagan T., Bar-Zvi D.
Plant Physiol. 101:1397-1398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Etiolated seedling.
[2]"A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein."
Kaldenhoff R., Koelling A., Richter G.
Plant Mol. Biol. 23:1187-1198(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. C24.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-286.
Strain: cv. Columbia.
Tissue: Seedling.
[8]"Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency."
Cutler S.R., Ehrhardt D.W., Griffitts J.S., Somerville C.R.
Proc. Natl. Acad. Sci. U.S.A. 97:3718-3723(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"From genome to function: the Arabidopsis aquaporins."
Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.
Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[10]"Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins."
Tournaire-Roux C., Sutka M., Javot H., Gout E., Gerbeau P., Luu D.-T., Bligny R., Maurel C.
Nature 425:393-397(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Methylation of aquaporins in plant plasma membrane."
Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C.
Biochem. J. 400:189-197(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68293 mRNA. Translation: CAA48356.1.
Z17424 mRNA. Translation: CAB37860.1.
AC004665 Genomic DNA. Translation: AAC28529.1.
CP002685 Genomic DNA. Translation: AEC10622.1.
AY063931 mRNA. Translation: AAL36287.1.
AY048294 mRNA. Translation: AAK82556.1.
AY091254 mRNA. Translation: AAM14193.1.
AY084470 mRNA. Translation: AAM61041.1.
Z25965 mRNA. Translation: CAA81120.1.
PIRT02451.
RefSeqNP_182120.1. NM_130159.3.
UniGeneAt.20518.
At.24183.

3D structure databases

ProteinModelPortalQ06611.
SMRQ06611. Positions 43-276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4540. 10 interactions.
IntActQ06611. 9 interactions.

Proteomic databases

PaxDbQ06611.
PRIDEQ06611.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G45960.1; AT2G45960.1; AT2G45960. [Q06611-1]
GeneID819204.
KEGGath:AT2G45960.

Organism-specific databases

GeneFarm4863. 182.
TAIRAT2G45960.

Phylogenomic databases

eggNOGCOG0580.
HOGENOMHOG000288286.
InParanoidQ06611.
KOK09872.
PhylomeDBQ06611.
ProtClustDBCLSN2682869.

Gene expression databases

ArrayExpressQ06611.
GenevestigatorQ06611.

Family and domain databases

Gene3D1.20.1080.10. 1 hit.
InterProIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERPTHR19139. PTHR19139. 1 hit.
PfamPF00230. MIP. 1 hit.
[Graphical view]
PRINTSPR00783. MINTRINSICP.
SUPFAMSSF81338. SSF81338. 1 hit.
TIGRFAMsTIGR00861. MIP. 1 hit.
PROSITEPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePIP12_ARATH
AccessionPrimary (citable) accession number: Q06611
Secondary accession number(s): Q8L5V4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names