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Protein

Aquaporin PIP1-2

Gene

PIP1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Water channel required to facilitate the transport of water across cell membrane. Essential for the water permeability of the plasma membrane and for the morphology of the root system. Its function is impaired by Hg2+. Inhibited by cytosolic acidosis which occurs during anoxia in roots.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-ATH-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin PIP1-2
Short name:
AtPIP1;2
Alternative name(s):
Plasma membrane intrinsic protein 1b
Short name:
PIP1b
Transmembrane protein A
Short name:
AthH2
Short name:
TMP-A
Gene namesi
Name:PIP1-2
Synonyms:PIP1B, TMPA
Ordered Locus Names:At2g45960
ORF Names:F4I18.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G45960.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicSequence analysisAdd BLAST54
Transmembranei55 – 75Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini76 – 81ExtracellularSequence analysis6
Transmembranei82 – 102Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini103 – 132CytoplasmicSequence analysisAdd BLAST30
Transmembranei133 – 153Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini154 – 174ExtracellularSequence analysisAdd BLAST21
Transmembranei175 – 195Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini196 – 208CytoplasmicSequence analysisAdd BLAST13
Transmembranei209 – 229Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini230 – 256ExtracellularSequence analysisAdd BLAST27
Transmembranei257 – 277Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini278 – 286CytoplasmicSequence analysis9

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000640471 – 286Aquaporin PIP1-2Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei284PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ06611.
PRIDEiQ06611.

PTM databases

SwissPalmiQ06611.

Expressioni

Tissue specificityi

Widely expressed. Expressed in roots and above ground.1 Publication

Inductioni

By blue light and abscisic acid (ABA).

Gene expression databases

ExpressionAtlasiQ06611. baseline and differential.
GenevisibleiQ06611. AT.

Interactioni

Protein-protein interaction databases

BioGridi4540. 34 interactors.
IntActiQ06611. 9 interactors.
STRINGi3702.AT3G61430.1.

Structurei

3D structure databases

ProteinModelPortaliQ06611.
SMRiQ06611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi114 – 116NPA 13
Motifi235 – 237NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiQ06611.
KOiK09872.
PhylomeDBiQ06611.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q06611-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPLF EPGELASWSF
60 70 80 90 100
WRAGIAEFIA TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA
110 120 130 140 150
LVYCTAGISG GHINPAVTFG LFLARKLSLT RAVYYIVMQC LGAICGAGVV
160 170 180 190 200
KGFQPKQYQA LGGGANTIAH GYTKGSGLGA EIIGTFVLVY TVFSATDAKR
210 220 230 240 250
NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL GAAIIFNKDN
260 270 280
AWDDHWVFWV GPFIGAALAA LYHVIVIRAI PFKSRS
Length:286
Mass (Da):30,598
Last modified:November 1, 1995 - v1
Checksum:i80F12775B6809013
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29Missing in AAM61041 (Ref. 6) Curated1
Sequence conflicti40F → L in CAB37860 (PubMed:8292783).Curated1
Sequence conflicti279A → T in AAM61041 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68293 mRNA. Translation: CAA48356.1.
Z17424 mRNA. Translation: CAB37860.1.
AC004665 Genomic DNA. Translation: AAC28529.1.
CP002685 Genomic DNA. Translation: AEC10622.1.
AY063931 mRNA. Translation: AAL36287.1.
AY048294 mRNA. Translation: AAK82556.1.
AY091254 mRNA. Translation: AAM14193.1.
AY084470 mRNA. Translation: AAM61041.1.
Z25965 mRNA. Translation: CAA81120.1.
PIRiT02451.
RefSeqiNP_182120.1. NM_130159.4. [Q06611-1]
UniGeneiAt.20518.
At.24183.

Genome annotation databases

EnsemblPlantsiAT2G45960.1; AT2G45960.1; AT2G45960. [Q06611-1]
GeneIDi819204.
GrameneiAT2G45960.1; AT2G45960.1; AT2G45960.
KEGGiath:AT2G45960.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68293 mRNA. Translation: CAA48356.1.
Z17424 mRNA. Translation: CAB37860.1.
AC004665 Genomic DNA. Translation: AAC28529.1.
CP002685 Genomic DNA. Translation: AEC10622.1.
AY063931 mRNA. Translation: AAL36287.1.
AY048294 mRNA. Translation: AAK82556.1.
AY091254 mRNA. Translation: AAM14193.1.
AY084470 mRNA. Translation: AAM61041.1.
Z25965 mRNA. Translation: CAA81120.1.
PIRiT02451.
RefSeqiNP_182120.1. NM_130159.4. [Q06611-1]
UniGeneiAt.20518.
At.24183.

3D structure databases

ProteinModelPortaliQ06611.
SMRiQ06611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4540. 34 interactors.
IntActiQ06611. 9 interactors.
STRINGi3702.AT3G61430.1.

PTM databases

SwissPalmiQ06611.

Proteomic databases

PaxDbiQ06611.
PRIDEiQ06611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45960.1; AT2G45960.1; AT2G45960. [Q06611-1]
GeneIDi819204.
GrameneiAT2G45960.1; AT2G45960.1; AT2G45960.
KEGGiath:AT2G45960.

Organism-specific databases

TAIRiAT2G45960.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiQ06611.
KOiK09872.
PhylomeDBiQ06611.

Enzyme and pathway databases

ReactomeiR-ATH-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiQ06611.

Gene expression databases

ExpressionAtlasiQ06611. baseline and differential.
GenevisibleiQ06611. AT.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIP12_ARATH
AccessioniPrimary (citable) accession number: Q06611
Secondary accession number(s): Q8L5V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.