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Q06604 (BSP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein BSP1
Alternative name(s):
Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1
Gene names
Name:BSP1
Ordered Locus Names:YPR171W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cortical patch protein involved in endocytosis. May serve as an adapter to link INP52 and INP53 to the cortical actin cytoskeleton. Ref.4

Subunit structure

Interacts with CAP1, RVS167, SLA1, INP52 and INP53. The INP52 and INP53 C-terminal Sac1 domains are required for the binding with BSP1. Ref.3 Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein. Cytoplasmcytoskeletonactin patch. Note: Peripheral membrane protein in a PtdIns4P and PtdIns(4,5)P2 manner. Ref.3 Ref.4 Ref.5

Post-translational modification

Phosphorylated by CDC28. Ref.7

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Protein BSP1
PRO_0000228137

Amino acid modifications

Modified residue461Phosphoserine Ref.9
Modified residue791Phosphoserine Ref.11
Modified residue881Phosphoserine Ref.11
Modified residue1851Phosphoserine Ref.11
Modified residue2201Phosphoserine Ref.10
Modified residue3091Phosphoserine Ref.9
Modified residue3201Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q06604 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7A33A775FCA1F3CD

FASTA57664,666
        10         20         30         40         50         60 
MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR RADMMTGKNV 

        70         80         90        100        110        120 
EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD LEGSASQKEK KFLISEEDYL 

       130        140        150        160        170        180 
LLQKLKASQT YNDSNADKNL PSFEKGPRMP SRGRPRPREK EIITIQYDFE LPGRADIPSS 

       190        200        210        220        230        240 
SSSSSPPPLP TRRDHIKITD GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP 

       250        260        270        280        290        300 
PVSRSTKPAS FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK 

       310        320        330        340        350        360 
NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL AAHKTKPSIP 

       370        380        390        400        410        420 
PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN IKKEEEDSIP EAIKGIQNLK 

       430        440        450        460        470        480 
KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD INKLNDEIEA LSLRNNLKKR PPTAPQRKIS 

       490        500        510        520        530        540 
LPEALRKVEL MKKSKTEPVL ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS 

       550        560        570 
DKYTTSRDET VKETKPLVHP NKNRTRGPRR KLPTRV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A protein interaction map for cell polarity development."
Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. expand/collapse author list , Pringle J.R., Davis T.N., Fields S., Drubin D.G.
J. Cell Biol. 154:549-571(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CAP1; RVS167 AND SLA1, SUBCELLULAR LOCATION.
[4]"Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast."
Wicky S., Frischmuth S., Singer-Krueger B.
FEBS Lett. 537:35-41(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH INP52 AND INP53, SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-309 AND SER-320, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-88 AND SER-185, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25842 Genomic DNA. Translation: AAB68105.1.
BK006949 Genomic DNA. Translation: DAA11588.1.
PIRS59829.
RefSeqNP_015497.1. NM_001184268.1.

3D structure databases

ProteinModelPortalQ06604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36344. 69 interactions.
DIPDIP-2756N.
IntActQ06604. 21 interactions.
MINTMINT-547483.
STRING4932.YPR171W.

Proteomic databases

MaxQBQ06604.
PaxDbQ06604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR171W; YPR171W; YPR171W.
GeneID856301.
KEGGsce:YPR171W.

Organism-specific databases

CYGDYPR171w.
SGDS000006375. BSP1.

Phylogenomic databases

eggNOGNOG12793.
OMATRRISKF.
OrthoDBEOG7FFN3X.

Enzyme and pathway databases

BioCycYEAST:G3O-34298-MONOMER.

Gene expression databases

GenevestigatorQ06604.

Family and domain databases

ProtoNetSearch...

Other

NextBio981658.

Entry information

Entry nameBSP1_YEAST
AccessionPrimary (citable) accession number: Q06604
Secondary accession number(s): D6W4H2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD