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Q06604

- BSP1_YEAST

UniProt

Q06604 - BSP1_YEAST

Protein

Protein BSP1

Gene

BSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cortical patch protein involved in endocytosis. May serve as an adapter to link INP52 and INP53 to the cortical actin cytoskeleton.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD

    GO - Biological processi

    1. actin cortical patch localization Source: SGD
    2. endocytosis Source: UniProtKB-KW

    Keywords - Biological processi

    Endocytosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34298-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BSP1
    Alternative name(s):
    Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1
    Gene namesi
    Name:BSP1
    Ordered Locus Names:YPR171W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR171w.
    SGDiS000006375. BSP1.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein. Cytoplasmcytoskeletonactin patch
    Note: Peripheral membrane protein in a PtdIns4P and PtdIns(4,5)P2 manner.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD
    2. cellular bud neck Source: SGD
    3. cellular bud tip Source: SGD
    4. membrane Source: SGD
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Protein BSP1PRO_0000228137Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 461Phosphoserine2 Publications
    Modified residuei79 – 791Phosphoserine2 Publications
    Modified residuei88 – 881Phosphoserine2 Publications
    Modified residuei185 – 1851Phosphoserine2 Publications
    Modified residuei220 – 2201Phosphoserine2 Publications
    Modified residuei309 – 3091Phosphoserine2 Publications
    Modified residuei320 – 3201Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDC28.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ06604.
    PaxDbiQ06604.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06604.

    Interactioni

    Subunit structurei

    Interacts with CAP1, RVS167, SLA1, INP52 and INP53. The INP52 and INP53 C-terminal Sac1 domains are required for the binding with BSP1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P158912EBI-37047,EBI-2036
    INP52P509424EBI-37047,EBI-28834
    LSB3P436037EBI-37047,EBI-22980
    RVS167P397435EBI-37047,EBI-14500
    SDC25P0CF342EBI-37047,EBI-6315249
    SLA1P327906EBI-37047,EBI-17313
    YSC84P327936EBI-37047,EBI-24460

    Protein-protein interaction databases

    BioGridi36344. 69 interactions.
    DIPiDIP-2756N.
    IntActiQ06604. 21 interactions.
    MINTiMINT-547483.
    STRINGi4932.YPR171W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG12793.
    OMAiTRRISKF.
    OrthoDBiEOG7FFN3X.

    Sequencei

    Sequence statusi: Complete.

    Q06604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR    50
    RADMMTGKNV EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD 100
    LEGSASQKEK KFLISEEDYL LLQKLKASQT YNDSNADKNL PSFEKGPRMP 150
    SRGRPRPREK EIITIQYDFE LPGRADIPSS SSSSSPPPLP TRRDHIKITD 200
    GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP PVSRSTKPAS 250
    FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK 300
    NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL 350
    AAHKTKPSIP PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN 400
    IKKEEEDSIP EAIKGIQNLK KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD 450
    INKLNDEIEA LSLRNNLKKR PPTAPQRKIS LPEALRKVEL MKKSKTEPVL 500
    ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS DKYTTSRDET 550
    VKETKPLVHP NKNRTRGPRR KLPTRV 576
    Length:576
    Mass (Da):64,666
    Last modified:November 1, 1996 - v1
    Checksum:i7A33A775FCA1F3CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25842 Genomic DNA. Translation: AAB68105.1.
    BK006949 Genomic DNA. Translation: DAA11588.1.
    PIRiS59829.
    RefSeqiNP_015497.1. NM_001184268.1.

    Genome annotation databases

    EnsemblFungiiYPR171W; YPR171W; YPR171W.
    GeneIDi856301.
    KEGGisce:YPR171W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25842 Genomic DNA. Translation: AAB68105.1 .
    BK006949 Genomic DNA. Translation: DAA11588.1 .
    PIRi S59829.
    RefSeqi NP_015497.1. NM_001184268.1.

    3D structure databases

    ProteinModelPortali Q06604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36344. 69 interactions.
    DIPi DIP-2756N.
    IntActi Q06604. 21 interactions.
    MINTi MINT-547483.
    STRINGi 4932.YPR171W.

    Proteomic databases

    MaxQBi Q06604.
    PaxDbi Q06604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR171W ; YPR171W ; YPR171W .
    GeneIDi 856301.
    KEGGi sce:YPR171W.

    Organism-specific databases

    CYGDi YPR171w.
    SGDi S000006375. BSP1.

    Phylogenomic databases

    eggNOGi NOG12793.
    OMAi TRRISKF.
    OrthoDBi EOG7FFN3X.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34298-MONOMER.

    Miscellaneous databases

    NextBioi 981658.

    Gene expression databases

    Genevestigatori Q06604.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: INTERACTION WITH CAP1; RVS167 AND SLA1, SUBCELLULAR LOCATION.
    4. "Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast."
      Wicky S., Frischmuth S., Singer-Krueger B.
      FEBS Lett. 537:35-41(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH INP52 AND INP53, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: PHOSPHORYLATION BY CDC28.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-309 AND SER-320, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-88 AND SER-185, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBSP1_YEAST
    AccessioniPrimary (citable) accession number: Q06604
    Secondary accession number(s): D6W4H2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 704 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3