Protein BSP1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q06604 (BSP1_YEAST)

Last modified November 3, 2009. Version 67. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Protein BSP1
Alternative name(s):
Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1

Gene names

Name:	BSP1
Ordered Locus Names:	YPR171W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Cortical patch protein involved in endocytosis. May serve as an adapter to link INP52 and INP53 to the cortical actin cytoskeleton. Ref.3
Subunit structure	Interacts with CAP1, RVS167, SLA1, INP52 and INP53. The INP52 and INP53 C-terminal Sac1 domains are required for the binding with BSP1. Ref.3 Ref.2
Subcellular location	Cell membrane; Peripheral membrane protein. Cytoplasm › cytoskeleton › actin patch. Note: Peripheral membrane protein in a PtdIns₄P and PtdIns(4,5)P2 manner. Ref.3 Ref.2 Ref.4
Post-translational modification	Phosphorylated by CDC28. Ref.6 Ref.7 Ref.8 Ref.9
Miscellaneous	Present with 704 molecules/cell in log phase SD medium. Ref.5

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Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cortical patch localization Ref.3 Inferred from mutant phenotype. Source: SGD endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	actin cortical patch Ref.3 Inferred from direct assay. Source: SGD cellular bud neck Ref.3 Inferred from direct assay. Source: SGD cellular bud tip Ref.3 Inferred from direct assay. Source: SGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay. Source: SGD membrane fraction Ref.3 Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding, bridging Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
BOI1	P38041	1	EBI-37047,EBI-3719
INP52	P50942	4	EBI-37047,EBI-28834
LSB3	P43603	2	EBI-37047,EBI-22980
RVS167	P39743	2	EBI-37047,EBI-14500
VAM7	P32912	1	EBI-37047,EBI-20232
YSC84	P32793	2	EBI-37047,EBI-24460

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 576

576

Protein BSP1

PRO_0000228137

Amino acid modifications

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.7

Modified residue

183

Phosphoserine Ref.9

Modified residue

184

Phosphoserine Ref.9

Modified residue

185

Phosphoserine Ref.9

Modified residue

220

Phosphoserine Ref.9

Modified residue

293

Phosphothreonine Ref.9

Modified residue

309

Phosphoserine Ref.7

Modified residue

320

Phosphoserine Ref.7

Modified residue

434

Phosphoserine Ref.9

Modified residue

535

Phosphothreonine Ref.8

Modified residue

539

Phosphothreonine Ref.8

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Sequences

Sequence

Length

Mass (Da)

Tools

Q06604-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7A33A775FCA1F3CD
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FASTA

576

64,666

        10         20         30         40         50         60 
MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR RADMMTGKNV 

        70         80         90        100        110        120 
EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD LEGSASQKEK KFLISEEDYL 

       130        140        150        160        170        180 
LLQKLKASQT YNDSNADKNL PSFEKGPRMP SRGRPRPREK EIITIQYDFE LPGRADIPSS 

       190        200        210        220        230        240 
SSSSSPPPLP TRRDHIKITD GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP 

       250        260        270        280        290        300 
PVSRSTKPAS FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK 

       310        320        330        340        350        360 
NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL AAHKTKPSIP 

       370        380        390        400        410        420 
PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN IKKEEEDSIP EAIKGIQNLK 

       430        440        450        460        470        480 
KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD INKLNDEIEA LSLRNNLKKR PPTAPQRKIS 

       490        500        510        520        530        540 
LPEALRKVEL MKKSKTEPVL ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS 

       550        560        570 
DKYTTSRDET VKETKPLVHP NKNRTRGPRR KLPTRV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A protein interaction map for cell polarity development." Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. , Pringle J.R., Davis T.N., Fields S., Drubin D.G. J. Cell Biol. 154:549-571(2001) [PubMed: 11489916] [Abstract] Cited for: INTERACTION WITH CAP1; RVS167 AND SLA1, SUBCELLULAR LOCATION.
[3]	"Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast." Wicky S., Frischmuth S., Singer-Krueger B. FEBS Lett. 537:35-41(2003) [PubMed: 12606027] [Abstract] Cited for: FUNCTION, INTERACTION WITH INP52 AND INP53, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-309 AND SER-320, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; THR-535 AND THR-539, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-183; SER-184; SER-185; SER-220; THR-293 AND SER-434, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U25842 Genomic DNA. Translation: AAB68105.1.
PIR	S59829.
RefSeq	NP_015497.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2756N.
IntAct	Q06604. 18 interactions.
STRING	Q06604.
Genome annotation databases
Ensembl	YPR171W; YPR171W; YPR171W; Saccharomyces cerevisiae. [Genome view]
GeneID	856301.
GenomeReviews	Gene locus YPR171W in contig U00094_GR.
KEGG	sce:YPR171W.
NMPDR	fig\|4932.3.peg.6646.
Organism-specific databases
CYGD	YPR171w.
SGD	S000006375. BSP1.
Phylogenomic databases
HOGENOM	Q06604.
OMA	RASSENV.
Gene expression databases
ArrayExpress	Q06604.
Genevestigator	Q06604.
GermOnline	YPR171W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	981658.

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Entry information

Entry name

BSP1_YEAST

Accession

Primary (citable) accession number: Q06604

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents