Reviewed,
UniProtKB/Swiss-Prot Q06604 (BSP1_YEAST)
Last modified
November 3, 2009.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein BSP1 Alternative name(s): Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 576 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Cortical patch protein involved in endocytosis. May serve as an adapter to link INP52 and INP53 to the cortical actin cytoskeleton. Ref.3 |
| Subunit structure | Interacts with CAP1, RVS167, SLA1, INP52 and INP53. The INP52 and INP53 C-terminal Sac1 domains are required for the binding with BSP1. Ref.3 Ref.2 |
| Subcellular location | Cell membrane; Peripheral membrane protein. Cytoplasm › cytoskeleton › actin patch. Note: Peripheral membrane protein in a PtdIns4P and PtdIns(4,5)P2 manner. Ref.3 Ref.2 Ref.4 |
| Post-translational modification | |
| Miscellaneous | Present with 704 molecules/cell in log phase SD medium. Ref.5 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Endocytosis |
| Cellular component | Cell membrane Cytoplasm Cytoskeleton Membrane |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | actin cortical patch localization Ref.3 Inferred from mutant phenotype. Source: SGD endocytosisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | actin cortical patch Ref.3 Inferred from direct assay. Source: SGD cellular bud neck Ref.3Inferred from direct assay. Source: SGD cellular bud tip Ref.3Inferred from direct assay. Source: SGD extrinsic to membraneInferred from electronic annotation. Source: UniProtKB-SubCell mating projection tipInferred from direct assay. Source: SGD membrane fraction Ref.3Inferred from direct assay. Source: SGD plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | protein binding, bridging Ref.3 Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BOI1 | P38041 | 1 | EBI-37047,EBI-3719 | |
| INP52 | P50942 | 4 | EBI-37047,EBI-28834 | |
| LSB3 | P43603 | 2 | EBI-37047,EBI-22980 | |
| RVS167 | P39743 | 2 | EBI-37047,EBI-14500 | |
| VAM7 | P32912 | 1 | EBI-37047,EBI-20232 | |
| YSC84 | P32793 | 2 | EBI-37047,EBI-24460 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 576 | 576 | Protein BSP1 | PRO_0000228137 | |||||
Amino acid modifications | |||||||||
| Modified residue | 39 | 1 | Phosphoserine Ref.8 Ref.9 | ||||||
| Modified residue | 46 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 183 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 184 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 185 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 220 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 293 | 1 | Phosphothreonine Ref.9 | ||||||
| Modified residue | 309 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 320 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 434 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 535 | 1 | Phosphothreonine Ref.8 | ||||||
| Modified residue | 539 | 1 | Phosphothreonine Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "A protein interaction map for cell polarity development." Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. Drubin D.G.J. Cell Biol. 154:549-571(2001) [PubMed: 11489916] [Abstract] Cited for: INTERACTION WITH CAP1; RVS167 AND SLA1, SUBCELLULAR LOCATION. |
| [3] | "Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast." Wicky S., Frischmuth S., Singer-Krueger B. FEBS Lett. 537:35-41(2003) [PubMed: 12606027] [Abstract] Cited for: FUNCTION, INTERACTION WITH INP52 AND INP53, SUBCELLULAR LOCATION. |
| [4] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28. |
| [7] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-309 AND SER-320, MASS SPECTROMETRY. |
| [8] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; THR-535 AND THR-539, MASS SPECTROMETRY. |
| [9] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-183; SER-184; SER-185; SER-220; THR-293 AND SER-434, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U25842 Genomic DNA. Translation: AAB68105.1. | |
| PIR | S59829. |
| RefSeq | NP_015497.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2756N. |
| IntAct | Q06604. 18 interactions. |
| STRING | Q06604. |
Genome annotation databases | |
| Ensembl | YPR171W; YPR171W; YPR171W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856301. |
| GenomeReviews | Gene locus YPR171W in contig U00094_GR. |
| KEGG | sce:YPR171W. |
| NMPDR | fig|4932.3.peg.6646. |
Organism-specific databases | |
| CYGD | YPR171w. |
| SGD | S000006375. BSP1. |
Phylogenomic databases | |
| HOGENOM | Q06604. |
| OMA | RASSENV. |
Gene expression databases | |
| ArrayExpress | Q06604. |
| Genevestigator | Q06604. |
| GermOnline | YPR171W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 981658. |
Entry information
| Entry name | BSP1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q06604 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||

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