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Protein

Arsenical-resistance protein 3

Gene

ARR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane transporter that confers resistance to toxic metalloids by mediating extrusion of arsenite (As(III)) and antimonite (Sb(III)) out of cells. Displays low-affinity As(III)/H+ and Sb(III)/H+ exchange activity.8 Publications

Kineticsi

Vmax for arsenite is approximately 3 times higher than for antimonite.1 Publication

Manual assertion based on experiment ini

  1. KM=2 mM for arsenite export1 Publication
  2. KM=2 mM for antimonite export1 Publication

    GO - Molecular functioni

    • antimonite transmembrane transporter activity Source: SGD
    • antiporter activity Source: SGD
    • arsenite transmembrane transporter activity Source: SGD
    • organic acid transmembrane transporter activity Source: GO_Central

    GO - Biological processi

    • antimonite transport Source: SGD
    • arsenite transport Source: SGD
    • response to arsenic-containing substance Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Biological processi

    Antiport, Arsenical resistance, Ion transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34321-MONOMER.

    Protein family/group databases

    TCDBi2.A.59.1.1. the arsenical resistance-3 (acr3) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arsenical-resistance protein 3Curated
    Alternative name(s):
    Arsenic compounds resistance protein 31 Publication
    As(III)/H(+) and Sb(III)/H(+)antiporterCurated
    Gene namesi
    Name:ARR3Imported
    Synonyms:ACR31 Publication
    Ordered Locus Names:YPR201WImported
    ORF Names:P9677.2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPR201W.
    SGDiS000006405. ARR3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 34Cytoplasmic1 PublicationAdd BLAST34
    Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
    Topological domaini56 – 69Extracellular1 PublicationAdd BLAST14
    Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
    Topological domaini91 – 113Cytoplasmic1 PublicationAdd BLAST23
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Topological domaini135 – 141Extracellular1 Publication7
    Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
    Topological domaini163 – 169Cytoplasmic1 Publication7
    Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
    Topological domaini191 – 216Extracellular1 PublicationAdd BLAST26
    Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
    Topological domaini238 – 245Cytoplasmic1 Publication8
    Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
    Topological domaini267 – 280Extracellular1 PublicationAdd BLAST14
    Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
    Topological domaini302 – 343Cytoplasmic1 PublicationAdd BLAST42
    Transmembranei344 – 364HelicalSequence analysisAdd BLAST21
    Topological domaini365 – 369Extracellular1 Publication5
    Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
    Topological domaini391 – 404Cytoplasmic1 PublicationAdd BLAST14

    GO - Cellular componenti

    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Biotechnological usei

    Heterologous expression endows plants with greater arsenic resistance by enhancing arsenite efflux. Reduces arsenic accumulation in rice grains (PubMed:22107880). Does not lower significantly arsenic tissue levels in Arabidopsis (PubMed:22380876).2 Publications

    Disruption phenotypei

    Leads to sensitivity to antimony, tellurite, cadmium, and phenylarsine oxide.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi90C → A: Leads to ER retention and arsenite sensitivity. 1 Publication1
    Mutagenesisi117N → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication1
    Mutagenesisi150R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi151C → V: Leads to complete loss of metalloid transport function. 1 Publication1
    Mutagenesisi158W → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication1
    Mutagenesisi169C → A: Greatly reduces arsenite efflux. 1 Publication1
    Mutagenesisi176N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi192C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication1
    Mutagenesisi230R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi266F → A: Impairs arsenite resistance. 1 Publication1
    Mutagenesisi283C → A: Does not alter the arsenite/proton exchange across the plasma membrane. 1 Publication1
    Mutagenesisi290Y → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi316C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication1
    Mutagenesisi318C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication1
    Mutagenesisi333C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication1
    Mutagenesisi344C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication1
    Mutagenesisi345F → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi349S → A: Impairs arsenite resistance. 1 Publication1
    Mutagenesisi351N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication1
    Mutagenesisi352F → A: Impairs arsenite resistance. 1 Publication1
    Mutagenesisi353E → A: Impairs arsenite resistance. 1 Publication1
    Mutagenesisi380E → A: Impairs arsenite resistance. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000644401 – 404Arsenical-resistance protein 3Add BLAST404

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi201N-linked (GlcNAc...)PROSITE-ProRule annotation1
    Glycosylationi365N-linked (GlcNAc...)PROSITE-ProRule annotation1

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    Expression is highly induced by arsenite and antimonite.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi36371. 21 interactors.
    DIPiDIP-3797N.
    IntActiQ06598. 3 interactors.
    MINTiMINT-560243.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06598.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    InParanoidiQ06598.
    KOiK03325.
    OMAiVVDITMA.
    OrthoDBiEOG092C3QM8.

    Family and domain databases

    InterProiIPR004706. Arsenical-R_Acr3.
    IPR002657. BilAc:Na_symport/Acr3.
    [Graphical view]
    PANTHERiPTHR10361. PTHR10361. 2 hits.
    PTHR10361:SF31. PTHR10361:SF31. 2 hits.
    PfamiPF01758. SBF. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005508. Acr3. 1 hit.
    TIGRFAMsiTIGR00832. acr3. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q06598-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEDQKSENS VPSKVNMVNR TDILTTIKSL SWLDLMLPFT IILSIIIAVI
    60 70 80 90 100
    ISVYVPSSRH TFDAEGHPNL MGVSIPLTVG MIVMMIPPIC KVSWESIHKY
    110 120 130 140 150
    FYRSYIRKQL ALSLFLNWVI GPLLMTALAW MALFDYKEYR QGIIMIGVAR
    160 170 180 190 200
    CIAMVLIWNQ IAGGDNDLCV VLVITNSLLQ MVLYAPLQIF YCYVISHDHL
    210 220 230 240 250
    NTSNRVLFEE VAKSVGVFLG IPLGIGIIIR LGSLTIAGKS NYEKYILRFI
    260 270 280 290 300
    SPWAMIGFHY TLFVIFISRG YQFIHEIGSA ILCFVPLVLY FFIAWFLTFA
    310 320 330 340 350
    LMRYLSISRS DTQRECSCDQ ELLLKRVWGR KSCEASFSIT MTQCFTMASN
    360 370 380 390 400
    NFELSLAIAI SLYGNNSKQA IAATFGPLLE VPILLILAIV ARILKPYYIW

    NNRN
    Length:404
    Mass (Da):45,848
    Last modified:November 1, 1996 - v1
    Checksum:i1BE290E26772B4CA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U25841 Genomic DNA. Translation: AAB64629.1.
    BK006949 Genomic DNA. Translation: DAA11615.1.
    PIRiS58830.
    RefSeqiNP_015527.1. NM_001184298.1.

    Genome annotation databases

    EnsemblFungiiYPR201W; YPR201W; YPR201W.
    GeneIDi856331.
    KEGGisce:YPR201W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U25841 Genomic DNA. Translation: AAB64629.1.
    BK006949 Genomic DNA. Translation: DAA11615.1.
    PIRiS58830.
    RefSeqiNP_015527.1. NM_001184298.1.

    3D structure databases

    ProteinModelPortaliQ06598.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi36371. 21 interactors.
    DIPiDIP-3797N.
    IntActiQ06598. 3 interactors.
    MINTiMINT-560243.

    Protein family/group databases

    TCDBi2.A.59.1.1. the arsenical resistance-3 (acr3) family.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYPR201W; YPR201W; YPR201W.
    GeneIDi856331.
    KEGGisce:YPR201W.

    Organism-specific databases

    EuPathDBiFungiDB:YPR201W.
    SGDiS000006405. ARR3.

    Phylogenomic databases

    InParanoidiQ06598.
    KOiK03325.
    OMAiVVDITMA.
    OrthoDBiEOG092C3QM8.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34321-MONOMER.

    Miscellaneous databases

    PROiQ06598.

    Family and domain databases

    InterProiIPR004706. Arsenical-R_Acr3.
    IPR002657. BilAc:Na_symport/Acr3.
    [Graphical view]
    PANTHERiPTHR10361. PTHR10361. 2 hits.
    PTHR10361:SF31. PTHR10361:SF31. 2 hits.
    PfamiPF01758. SBF. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005508. Acr3. 1 hit.
    TIGRFAMsiTIGR00832. acr3. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiARR3_YEAST
    AccessioniPrimary (citable) accession number: Q06598
    Secondary accession number(s): D6W4J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.