Q06584 (PYS2_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyocin-S2
EC=3.1.-.-

Alternative name(s):
Killer protein

Gene names

Name:	pys2
Ordered Locus Names:	PA1150

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells.
Subunit structure	Purified pyocin S2 makes up a complex of the two (large and small) proteins. The large protein, but not the pyocin complex, shows in vitro DNase activity.
Miscellaneous	Pyocins contain N-terminal receptor-binding domain, translocation domain and C-terminal DNase domain.
Sequence similarities	Belongs to the colicin/pyosin nuclease family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Antibiotic Antimicrobial Bacteriocin Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: InterPro
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 689	688	Pyocin-S2		PRO_0000218686
Sites
	Metal binding	656	1	Zinc By similarity
	Metal binding	681	1	Zinc By similarity
	Metal binding	685	1	Zinc By similarity
Experimental info
	Sequence conflict	605	1	R → RR AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q06584 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3235FAB8592D0EF0	FASTA	689	73,854
	10 20 30 40 50 60 MAVNDYEPGS MVITHVQGGG RDIIQYIPAR SSYGTPPFVP PGPSPYVGTG MQEYRKLRST 70 80 90 100 110 120 LDKSHSELKK NLKNETLKEV DELKSEAGLP GKAVSANDIR DEKSIVDALM DAKAKSLKAI 130 140 150 160 170 180 EDRPANLYTA SDFPQKSESM YQSQLLASRK FYGEFLDRHM SELAKAYSAD IYKAQIAILK 190 200 210 220 230 240 QTSQELENKA RSLEAEAQRA AAEVEADYKA RKANVEKKVQ SELDQAGNAL PQLTNPTPEQ 250 260 270 280 290 300 WLERATQLVT QAIANKKKLQ TANNALIAKA PNALEKQKAT YNADLLVDEI ASLQARLDKL 310 320 330 340 350 360 NAETARRKEI ARQAAIRAAN TYAMPANGSV VATAAGRGLI QVAQGAASLA QAISDAIAVL 370 380 390 400 410 420 GRVLASAPSV MAVGFASLTY SSRTAEQWQD QTPDSVRYAL GMDAAKLGLP PSVNLNAVAK 430 440 450 460 470 480 ASGTVDLPMR LTNEARGNTT TLSVVSTDGV SVPKAVPVRM AAYNATTGLY EVTVPSTTAE 490 500 510 520 530 540 APPLILTWTP ASPPGNQNPS STTPVVPKPV PVYEGATLTP VKATPETYPG VITLPEDLII 550 560 570 580 590 600 GFPADSGIKP IYVMFRDPRD VPGAATGKGQ PVSGNWLGAA SQGEGAPIPS QIADKLRGKT 610 620 630 640 650 660 FKNWRDFREQ FWIAVANDPE LSKQFNPGSL AVMRDGGAPY VRESEQAGGR IKIEIHHKVR 670 680 IADGGGVYNM GNLVAVTPKR HIEIHKGGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular structures and functions of pyocins S1 and S2 in Pseudomonas aeruginosa." Sano Y., Matsui H., Kobayashi M., Kageyama M. J. Bacteriol. 175:2907-2916(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-18. Strain: PAO.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12708 Genomic DNA. Translation: BAA02203.1. AE004091 Genomic DNA. Translation: AAG04539.1.
PIR	C36907. D83501.
RefSeq	NP_249841.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q06584.
SMR	Q06584. Positions 559-687.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA1150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878080.
KEGG	pae:PA1150.
PATRIC	19836620. VBIPseAer58763_1196.
Organism-specific databases
PseudoCAP	PA1150.
Phylogenomic databases
eggNOG	NOG13622.
HOGENOM	HOG000081584.
OMA	WLERATQ.
ProtClustDB	CLSK2392921.
Family and domain databases
Gene3D	3.90.540.10. 1 hit.
InterPro	IPR024622. Colicin/pyocin_DNase_dom. IPR003615. HNH_nuc. IPR003060. Pyocin_killer. IPR016128. Pyosin/cloacin_T_dom. [Graphical view]
Pfam	PF03515. Cloacin. 1 hit. PF12639. Colicin-DNase. 1 hit. PF06958. Pyocin_S. 1 hit. [Graphical view]
PRINTS	PR01300. PYOCINKILLER.
SMART	SM00507. HNHc. 1 hit. [Graphical view]
SUPFAM	SSF69369. Pyoson/cloacin_translocation. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PYS2_PSEAE

Accession

Primary (citable) accession number: Q06584

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program