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Q06580 (MLC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin light chain 2
Alternative name(s):
Calmodulin-like myosin light chain MLC2
MYO1 light chain 2
Myosin-1 light chain
Gene names
Name:MLC2
Ordered Locus Names:YPR188C
ORF Names:P9677.10
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory light chain for the class II conventional myosin MYO1. May play a role in the disassembly of the MYO1 ring at the bud neck at the end of its contraction during cytokinesis. Ref.4

Subunit structure

Interacts with the IQ domain of MYO1. Ref.4

Subcellular location

Bud neck. Note: Forms a ring at the bud neck in a MYO1-dependent manner. Ref.4 Ref.5

Miscellaneous

Present with 1127 molecules/cell in log phase SD medium.

This chain binds calcium Potential.

Sequence similarities

Contains 2 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163Myosin light chain 2
PRO_0000198766

Regions

Domain15 – 5036EF-hand 1
Domain92 – 12736EF-hand 2
Calcium binding28 – 3912 Potential

Sequences

Sequence LengthMass (Da)Tools
Q06580 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6BAE6EA0ADF66B9B

FASTA16318,521
        10         20         30         40         50         60 
MDHSESLTFN QLTQDYINKL KDAFQMLDED EDGLISRGDL TKIYATLGKT LTDEEWSKMV 

        70         80         90        100        110        120 
PDNDTSTAEV GEEGVSFPIF LSIMGKNLSQ FPEREELEES LKAIGRGHDL NVPLNEVIDS 

       130        140        150        160 
LKEAGFENPE EEFAKLFKLF TTNQQATEER TFRGKLFLDS ITD 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae."
Luo J., Vallen E.A., Dravis C., Tcheperegine S.E., Drees B., Bi E.
J. Cell Biol. 165:843-855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MYO1.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25841 Genomic DNA. Translation: AAB64617.1.
AY558057 Genomic DNA. Translation: AAS56383.1.
BK006949 Genomic DNA. Translation: DAA11604.1.
PIRS58818.
RefSeqNP_015514.1. NM_001184285.1.

3D structure databases

ProteinModelPortalQ06580.
SMRQ06580. Positions 7-123.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36360. 22 interactions.
DIPDIP-2767N.
IntActQ06580. 7 interactions.
MINTMINT-514040.
STRING4932.YPR188C.

Proteomic databases

PaxDbQ06580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR188C; YPR188C; YPR188C.
GeneID856318.
KEGGsce:YPR188C.

Organism-specific databases

CYGDYPR188c.
SGDS000006392. MLC2.

Phylogenomic databases

eggNOGCOG5126.
HOGENOMHOG000066016.
OMAQATEERT.
OrthoDBEOG73FR0X.

Enzyme and pathway databases

BioCycYEAST:G3O-34311-MONOMER.

Gene expression databases

GenevestigatorQ06580.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio981706.

Entry information

Entry nameMLC2_YEAST
AccessionPrimary (citable) accession number: Q06580
Secondary accession number(s): D6W4I8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families