Q06580 (MLC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain 2

Alternative name(s):
Calmodulin-like myosin light chain MLC2
MYO1 light chain 2
Myosin-1 light chain

Gene names

Name:	MLC2
Ordered Locus Names:	YPR188C
ORF Names:	P9677.10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory light chain for the class II conventional myosin MYO1. May play a role in the disassembly of the MYO1 ring at the bud neck at the end of its contraction during cytokinesis. Ref.4
Subunit structure	Interacts with the IQ domain of MYO1. Ref.4
Subcellular location	Bud neck. Note: Forms a ring at the bud neck in a MYO1-dependent manner. Ref.4 Ref.5
Miscellaneous	Present with 1127 molecules/cell in log phase SD medium. This chain binds calcium Potential.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Motor protein Myosin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actomyosin contractile ring contraction Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	cellular bud neck contractile ring Inferred from direct assay Ref.4. Source: SGD myosin complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Myosin light chain 2		PRO_0000198766
Regions
	Domain	15 – 50	36	EF-hand 1
	Domain	92 – 127	36	EF-hand 2
	Calcium binding	28 – 39	12	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06580 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6BAE6EA0ADF66B9B	FASTA	163	18,521
	10 20 30 40 50 60 MDHSESLTFN QLTQDYINKL KDAFQMLDED EDGLISRGDL TKIYATLGKT LTDEEWSKMV 70 80 90 100 110 120 PDNDTSTAEV GEEGVSFPIF LSIMGKNLSQ FPEREELEES LKAIGRGHDL NVPLNEVIDS 130 140 150 160 LKEAGFENPE EEFAKLFKLF TTNQQATEER TFRGKLFLDS ITD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae." Luo J., Vallen E.A., Dravis C., Tcheperegine S.E., Drees B., Bi E. J. Cell Biol. 165:843-855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MYO1.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25841 Genomic DNA. Translation: AAB64617.1. AY558057 Genomic DNA. Translation: AAS56383.1. BK006949 Genomic DNA. Translation: DAA11604.1.
PIR	S58818.
RefSeq	NP_015514.1. NM_001184285.1.
3D structure databases
ProteinModelPortal	Q06580.
SMR	Q06580. Positions 7-123.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2767N.
IntAct	Q06580. 8 interactions.
MINT	MINT-514040.
STRING	4932.YPR188C.
Proteomic databases
PaxDb	Q06580.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR188C; YPR188C; YPR188C.
GeneID	856318.
KEGG	sce:YPR188C.
Organism-specific databases
CYGD	YPR188c.
SGD	S000006392. MLC2.
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000066016.
OMA	QATEERT.
OrthoDB	EOG4DRDNQ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-34311-MONOMER.
Gene expression databases
Genevestigator	Q06580.
GermOnline	YPR188C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	981706.

Entry information

Entry name

MLC2_YEAST

Accession

Primary (citable) accession number: Q06580
Secondary accession number(s): D6W4I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program