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Q06580

- MLC2_YEAST

UniProt

Q06580 - MLC2_YEAST

Protein

Myosin light chain 2

Gene

MLC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Regulatory light chain for the class II conventional myosin MYO1. May play a role in the disassembly of the MYO1 ring at the bud neck at the end of its contraction during cytokinesis.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi28 – 3912PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. myosin II binding Source: SGD

    GO - Biological processi

    1. actomyosin contractile ring contraction Source: SGD

    Keywords - Molecular functioni

    Motor protein, Myosin

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34311-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain 2
    Alternative name(s):
    Calmodulin-like myosin light chain MLC2
    MYO1 light chain 2
    Myosin-1 light chain
    Gene namesi
    Name:MLC2
    Ordered Locus Names:YPR188C
    ORF Names:P9677.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR188c.
    SGDiS000006392. MLC2.

    Subcellular locationi

    Bud neck 2 Publications
    Note: Forms a ring at the bud neck in a MYO1-dependent manner.

    GO - Cellular componenti

    1. cellular bud neck contractile ring Source: SGD
    2. myosin II complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 163163Myosin light chain 2PRO_0000198766Add
    BLAST

    Proteomic databases

    MaxQBiQ06580.
    PaxDbiQ06580.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06580.

    Interactioni

    Subunit structurei

    Interacts with the IQ domain of MYO1.1 Publication

    Protein-protein interaction databases

    BioGridi36360. 22 interactions.
    DIPiDIP-2767N.
    IntActiQ06580. 7 interactions.
    MINTiMINT-514040.
    STRINGi4932.YPR188C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06580.
    SMRiQ06580. Positions 7-123.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 5036EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini92 – 12736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    HOGENOMiHOG000066016.
    OMAiQATEERT.
    OrthoDBiEOG73FR0X.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06580-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDHSESLTFN QLTQDYINKL KDAFQMLDED EDGLISRGDL TKIYATLGKT    50
    LTDEEWSKMV PDNDTSTAEV GEEGVSFPIF LSIMGKNLSQ FPEREELEES 100
    LKAIGRGHDL NVPLNEVIDS LKEAGFENPE EEFAKLFKLF TTNQQATEER 150
    TFRGKLFLDS ITD 163
    Length:163
    Mass (Da):18,521
    Last modified:November 1, 1996 - v1
    Checksum:i6BAE6EA0ADF66B9B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25841 Genomic DNA. Translation: AAB64617.1.
    AY558057 Genomic DNA. Translation: AAS56383.1.
    BK006949 Genomic DNA. Translation: DAA11604.1.
    PIRiS58818.
    RefSeqiNP_015514.1. NM_001184285.1.

    Genome annotation databases

    EnsemblFungiiYPR188C; YPR188C; YPR188C.
    GeneIDi856318.
    KEGGisce:YPR188C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25841 Genomic DNA. Translation: AAB64617.1 .
    AY558057 Genomic DNA. Translation: AAS56383.1 .
    BK006949 Genomic DNA. Translation: DAA11604.1 .
    PIRi S58818.
    RefSeqi NP_015514.1. NM_001184285.1.

    3D structure databases

    ProteinModelPortali Q06580.
    SMRi Q06580. Positions 7-123.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36360. 22 interactions.
    DIPi DIP-2767N.
    IntActi Q06580. 7 interactions.
    MINTi MINT-514040.
    STRINGi 4932.YPR188C.

    Proteomic databases

    MaxQBi Q06580.
    PaxDbi Q06580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR188C ; YPR188C ; YPR188C .
    GeneIDi 856318.
    KEGGi sce:YPR188C.

    Organism-specific databases

    CYGDi YPR188c.
    SGDi S000006392. MLC2.

    Phylogenomic databases

    eggNOGi COG5126.
    HOGENOMi HOG000066016.
    OMAi QATEERT.
    OrthoDBi EOG73FR0X.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34311-MONOMER.

    Miscellaneous databases

    NextBioi 981706.

    Gene expression databases

    Genevestigatori Q06580.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae."
      Luo J., Vallen E.A., Dravis C., Tcheperegine S.E., Drees B., Bi E.
      J. Cell Biol. 165:843-855(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MYO1.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMLC2_YEAST
    AccessioniPrimary (citable) accession number: Q06580
    Secondary accession number(s): D6W4I8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1127 molecules/cell in log phase SD medium.1 Publication
    This chain binds calcium.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3