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Q06572 (AVP_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyrophosphate-energized vacuolar membrane proton pump
EC=3.6.1.1
Alternative name(s):
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Contributes to the transtonoplast (from cytosol to vacuole lumen) H+-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H+-ATPase on the same membrane.

Catalytic activity

Diphosphate + H2O = 2 phosphate.

Subunit structure

Monomer By similarity.

Subcellular location

Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

vacuolar membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 762762Pyrophosphate-energized vacuolar membrane proton pump
PRO_0000217042

Regions

Topological domain1 – 1010Cytoplasmic Potential
Transmembrane11 – 3222Helical; Potential
Topological domain33 – 9159Intravacuolar Potential
Transmembrane92 – 11120Helical; Potential
Topological domain112 – 13120Cytoplasmic Potential
Transmembrane132 – 15524Helical; Potential
Topological domain156 – 18429Intravacuolar Potential
Transmembrane185 – 20521Helical; Potential
Topological domain206 – 21712Cytoplasmic Potential
Transmembrane218 – 23720Helical; Potential
Topological domain238 – 31679Intravacuolar Potential
Transmembrane317 – 33721Helical; Potential
Topological domain338 – 36023Cytoplasmic Potential
Transmembrane361 – 38222Helical; Potential
Topological domain383 – 39816Intravacuolar Potential
Transmembrane399 – 41820Helical; Potential
Topological domain419 – 45436Cytoplasmic Potential
Transmembrane455 – 47824Helical; Potential
Topological domain479 – 53456Intravacuolar Potential
Transmembrane535 – 55622Helical; Potential
Topological domain557 – 57014Cytoplasmic Potential
Transmembrane571 – 59020Helical; Potential
Topological domain591 – 64959Intravacuolar
Transmembrane650 – 67324Helical; Potential
Topological domain674 – 76289Cytoplasmic Potential
Compositional bias57 – 604Poly-Glu

Sites

Active site2791Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06572 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: 90CA2A2532D2C910

FASTA76279,533
        10         20         30         40         50         60 
MAILGELGTE ILIPVCGVIG IVFAVAQWFI VSKVKVTPGA ASAAAGAKNG YGDYLIEEEE 

        70         80         90        100        110        120 
GLNDHNVVVK CAEIQTAISE GATSFLFTMY QYVGMFMVVF AAIIFLFLGS IEGFSTKGQP 

       130        140        150        160        170        180 
CTYSKGTCKP ALYTALFSTA SFLLGAITSL VSGFLGMKIA TYANARTTLE ARKGVGKAFI 

       190        200        210        220        230        240 
TAFRSGAVMG FLLSSSGLVV LYITINVFKM YYGDDWEGLF ESITGYGLGG SSMALFGRVG 

       250        260        270        280        290        300 
GGIYTKAADV GADLVGKVER NIPEDDPRNP AVIADNVGDN VGDIAGMGSD LFGSYAESSC 

       310        320        330        340        350        360 
AALVVASISS FGINHDFTAM CYPLLVSSVG IIVCLLTTLF ATDFFEIKAA NEIEPALKKQ 

       370        380        390        400        410        420 
LIISTALMTV GVAVISWLAL PAKFTIFNFG AQKEVSNWGL FFCVAVGLWA GLIIGFVTEY 

       430        440        450        460        470        480 
YTSNAYSPVQ DVADSCRTGA ATNVIFGLAL GYKSVIIPIF AIAVSIYVSF SIAAMYGIAM 

       490        500        510        520        530        540 
AALGMLSTMA TGLAIDAYGP ISDNAGGIAE MAGMSHRIRE RTDALDAAGN TTAAIGKGFA 

       550        560        570        580        590        600 
IGSAALVSLA LFGAFVSRAG VKVVDVLSPK VFIGLIVGAM LPYWFSAMTM KSVGSAALKM 

       610        620        630        640        650        660 
VEEVRRQFNT IPGLMEGTAK PDYATCVKIS TDASIKEMIP PGALVMLTPL IVGTLFGVET 

       670        680        690        700        710        720 
LSGVLAGALV SGVQIAISAS NTGGAWDNAK KYIEAGNSEH ARSLGPKGSD CHKAAVIGDT 

       730        740        750        760 
IGDPLKDTSG PSLNILIKLM AVESLVFAPF FATYGGLLFK YI

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References

[1]"Molecular cloning of cDNA for vacuolar membrane proton-translocating inorganic pyrophosphatase in Hordeum vulgare."
Tanaka Y., Chiba K., Maeda M., Maeshima M.
Biochem. Biophys. Res. Commun. 190:1110-1114(1993) [PubMed: 8382487] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Kashima.
Tissue: Root.
[2]Tanaka Y.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 41-45.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D13472 mRNA. Translation: BAA02717.2.
PIRJC1466.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ06572.

Gene expression databases

GenevestigatorQ06572.

Family and domain databases

InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAVP_HORVU
AccessionPrimary (citable) accession number: Q06572
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 14, 2001
Last modified: May 31, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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