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Protein

Uncharacterized ATP-dependent helicase IRC20

Gene

IRC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is probably involved in a pathway contributing to genomic integrity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi391 – 3988ATPPROSITE-ProRule annotation
Zinc fingeri1239 – 127739RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: SGD
  • ubiquitin-protein transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • double-strand break repair via nonhomologous end joining Source: SGD
  • double-strand break repair via synthesis-dependent strand annealing Source: SGD
  • protein ubiquitination Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32352-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized ATP-dependent helicase IRC20 (EC:3.6.1.-)
Alternative name(s):
Increased recombination centers protein 20
Gene namesi
Name:IRC20
Ordered Locus Names:YLR247C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR247C.
SGDiS000004237. IRC20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Displays increased levels of spontaneous RAD52 foci in proliferating diploid cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 15561555Uncharacterized ATP-dependent helicase IRC20PRO_0000268181Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei810 – 8101PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ06554.

PTM databases

iPTMnetiQ06554.

Expressioni

Inductioni

By heat shock and UV radiation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31515. 82 interactions.
DIPiDIP-6373N.
IntActiQ06554. 1 interaction.
MINTiMINT-700566.

Structurei

3D structure databases

ProteinModelPortaliQ06554.
SMRiQ06554. Positions 389-579, 1235-1285, 1378-1546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini378 – 583206Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1363 – 1531169Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1239 – 127739RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00730000111123.
InParanoidiQ06554.
KOiK15710.
OMAiYITQCAH.
OrthoDBiEOG7T1RKH.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVGALLAR EYNVTAEKCD FFLENGSFDS VIAALPALNQ EQETVTQKVS
60 70 80 90 100
KNGKELINVA SVNIEIPERI SLSNNGRQLF KFIVDIEILP CENDNEATLV
110 120 130 140 150
VSSDSVSLFQ IGFKMENNSK IAVDKQLPLI LDICAHKNQE RALRNQLENR
160 170 180 190 200
KSLERKPSRK RRKKNSNVND PEKLLKSRIH ELSLSYKDFE CSPVVFRYND
210 220 230 240 250
SSLTWVLSFN LKLKFLNNKF NRFSVEANQI LDLTFSNRDE NEFERYHKHS
260 270 280 290 300
HIHSNFIQKQ FISQILEYSK DRLSKIKPFL PQSIPDLKVN LLPFQRESVE
310 320 330 340 350
WMLIKEGHGN SLSDTPTVID EVGLIDFMNE YYAYGYELIA RSPDEVGPSL
360 370 380 390 400
LWNKLTGYIL TTEDAAHLYN QYRKERLSGD YPVCAKGVLA EEMGLGKTIE
410 420 430 440 450
ILSLILLNRR KLKDSEATFI DDENRTITKT KTTLIICPNA ILKQWLEEIE
460 470 480 490 500
LHANSLKWYT YRGYNEIMKD CKTVDEAVQQ LCQYDIIVTS YNIIATEVHH
510 520 530 540 550
AEFNRSIRSR RLKSPKYDYS SPLALMQFYR IILDEVQMLR SSSTYSAKCT
560 570 580 590 600
SLLHRIHTWG VSGTPIQNIY NFRMIMSYLK LHPFCDEVDF IRTLQEEIKL
610 620 630 640 650
RNEAKDYTSN DFVCQLKGVR FSIKDCMNIF YRYDLCIRHS KANVASQIHI
660 670 680 690 700
PRQHNFIIPL EFAPIEWDNY LNLWNNFLEL SGYNSDGSGS PRVSNAFLNE
710 720 730 740 750
WLSRLRYICC HALFPEILST RQKRLHGHLS RISNIDDILI SMRMDAFDSL
760 770 780 790 800
IGYYRERFHL SIKQAQYELE ISNTPAKALE SFIKIRDDLM IHIRQKFNVE
810 820 830 840 850
DPFDKSLNLS EDEDEHMDER FGEKETSSGD ESDREINGAK NHDNHNNDGM
860 870 880 890 900
LSNHLKKKGL RAMMNLLHDC YFFLGSVYYN LGTRKLEEAD DKHRKEKTEE
910 920 930 940 950
VVYSDVFPKN ELEEIEENRL LEQENYANAE ILRKSILSSE ARKVDMTIKM
960 970 980 990 1000
ARTKFAPMTS NIPLRLINIE FDHKNDYSSN LAVSRCFKSL SKLIEGLNEQ
1010 1020 1030 1040 1050
TKNFNELLDE LLIIIYEPVH RTEDDDSTNK IIGNEEYSTS IDSQDKIFSL
1060 1070 1080 1090 1100
LGCLEIILQN RDNILTSESE VKIPKHLVPE GSIISKYQKQ LLNSLRLISG
1110 1120 1130 1140 1150
TPLRTVFDEL KNSRIVRRIS SSNESESTIQ NFEDYLLQYE VESKSLFKYN
1160 1170 1180 1190 1200
KQVRESLKIL GSIYNAKTEY YSQLQRISDS LVSLHSLSAP QLSHLIRTIN
1210 1220 1230 1240 1250
KSLGGTLDAK INNIESRLIY LKNLSRLKDT LNDNQILSCS ICLGEVEIGA
1260 1270 1280 1290 1300
IIKCGHYFCK SCILTWLRAH SKCPICKGFC SISEVYNFKF KNSTEKREKE
1310 1320 1330 1340 1350
IQEPRREGAD SSQDNSNENS IISNMSEVEK LFGNKYEQFH QINEVHQIHI
1360 1370 1380 1390 1400
KESFGAKIDF VIKLISYLRL KSEQENADPP QVILYSQKTE YLKVIGKVLK
1410 1420 1430 1440 1450
LYHIEHLACL SNTANVGETI NNFKRQPSVT CLLLNVKTLG AGLNLINAKH
1460 1470 1480 1490 1500
IFLLDPILNN SDELQAMGRN NRIGQDEETF VWNFMIRNTV EENILRYKCI
1510 1520 1530 1540 1550
LEERKRKEKS KKGDKYDEAQ DETDNEESDD AKFEISVVDQ EVSNEHLWNC

FFHGSD
Length:1,556
Mass (Da):180,344
Last modified:November 1, 1996 - v1
Checksum:i6258491CE361EAC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67400.1.
BK006945 Genomic DNA. Translation: DAA09561.1.
PIRiS59393.
RefSeqiNP_013348.1. NM_001182134.1.

Genome annotation databases

EnsemblFungiiYLR247C; YLR247C; YLR247C.
GeneIDi850949.
KEGGisce:YLR247C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67400.1.
BK006945 Genomic DNA. Translation: DAA09561.1.
PIRiS59393.
RefSeqiNP_013348.1. NM_001182134.1.

3D structure databases

ProteinModelPortaliQ06554.
SMRiQ06554. Positions 389-579, 1235-1285, 1378-1546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31515. 82 interactions.
DIPiDIP-6373N.
IntActiQ06554. 1 interaction.
MINTiMINT-700566.

PTM databases

iPTMnetiQ06554.

Proteomic databases

MaxQBiQ06554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR247C; YLR247C; YLR247C.
GeneIDi850949.
KEGGisce:YLR247C.

Organism-specific databases

EuPathDBiFungiDB:YLR247C.
SGDiS000004237. IRC20.

Phylogenomic databases

GeneTreeiENSGT00730000111123.
InParanoidiQ06554.
KOiK15710.
OMAiYITQCAH.
OrthoDBiEOG7T1RKH.

Enzyme and pathway databases

BioCyciYEAST:G3O-32352-MONOMER.

Miscellaneous databases

NextBioi967413.
PROiQ06554.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
    Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
    Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination."
    Alvaro D., Lisby M., Rothstein R.
    PLoS Genet. 3:E228-E228(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-810, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRC20_YEAST
AccessioniPrimary (citable) accession number: Q06554
Secondary accession number(s): D6VYP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 143 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.