Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Palmitoyltransferase ERF2

Gene

ERF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The ERF2-SHR5 complex is a palmitoyltransferase specific for Ras proteins. Palmitoylates RAS2, which is required for its proper plasma membrane localization.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • protein targeting to membrane Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-32351-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ERF2 (EC:2.3.1.225)
Alternative name(s):
DHHC cysteine-rich domain-containing protein ERF2
Ras protein acyltransferase
Gene namesi
Name:ERF2
Ordered Locus Names:YLR246W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR246W
SGDiS000004236 ERF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 104LumenalSequence analysis8
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 217CytoplasmicSequence analysisAdd BLAST92
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Topological domaini239 – 250LumenalSequence analysisAdd BLAST12
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21
Topological domaini272 – 359CytoplasmicSequence analysisAdd BLAST88

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173K → E in ERF2-1; loss of function. 1 Publication1
Mutagenesisi182R → Q in ERF2-7; loss of function. 1 Publication1
Mutagenesisi189C → S: Abolishes palmitoyltransferase activity and interaction with SHR5. 2 Publications1
Mutagenesisi200D → A: Loss of function. 1 Publication1
Mutagenesisi201H → A: Abolishes palmitoyltransferase activity. 1 Publication1
Mutagenesisi203C → S: Abolishes palmitoyltransferase activity. 1 Publication1
Mutagenesisi218F → S in ERF2-2; loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129481 – 359Palmitoyltransferase ERF2Add BLAST359

Post-translational modificationi

Autopalmitoylated.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ06551
PRIDEiQ06551

Interactioni

Subunit structurei

Interacts with SHR5.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31513, 78 interactors
DIPiDIP-4783N
IntActiQ06551, 3 interactors
MINTiQ06551
STRINGi4932.YLR246W

Structurei

3D structure databases

ProteinModelPortaliQ06551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 223DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074293
HOGENOMiHOG000248576
InParanoidiQ06551
KOiK16675
OMAiRYFYLFT
OrthoDBiEOG092C4JRP

Family and domain databases

InterProiView protein in InterPro
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF01529 DHHC, 1 hit
PROSITEiView protein in PROSITE
PS50216 DHHC, 1 hit

Sequencei

Sequence statusi: Complete.

Q06551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVSRRSTR SESTSITKEE HTGEGSLTKL FFRWLVTLEG DQDINDGKGY
60 70 80 90 100
ISLPNVSNYI FFLGGRFRTV KGAKPLWLGV LLAIVCPMVL FSIFEAHKLW
110 120 130 140 150
HTQNGYKVLV IFFYYFWVIT LASFIRTATS DPGVLPRNIH LSQLRNNYQI
160 170 180 190 200
PQEYYNLITL PTHSSISKDI TIKYCPSCRI WRPPRSSHCS TCNVCVMVHD
210 220 230 240 250
HHCIWVNNCI GKRNYRFFLI FLLGAILSSV ILLTNCAIHI ARESGGPRDC
260 270 280 290 300
PVAILLLCYA GLTLWYPAIL FTYHIFMAGN QQTTREFLKG IGSKKNPVFH
310 320 330 340 350
RVVKEENIYN KGSFLKNMGH LMLEPRGPSF VSARKPHEAG DWRFMDLSPA

HSFEKIQKI
Length:359
Mass (Da):41,099
Last modified:November 1, 1996 - v1
Checksum:iB1FF42A2370F064B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68R → M in AAT92932 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA Translation: AAB67393.1
AY692913 Genomic DNA Translation: AAT92932.1
BK006945 Genomic DNA Translation: DAA09560.1
PIRiS59392
RefSeqiNP_013347.1, NM_001182133.1

Genome annotation databases

EnsemblFungiiYLR246W; YLR246W; YLR246W
GeneIDi850947
KEGGisce:YLR246W

Similar proteinsi

Entry informationi

Entry nameiERFB_YEAST
AccessioniPrimary (citable) accession number: Q06551
Secondary accession number(s): D6VYP4, Q6B217
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health