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Protein

Palmitoyltransferase ERF2

Gene

ERF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The ERF2-SHR5 complex is a palmitoyltransferase specific for Ras proteins. Palmitoylates RAS2, which is required for its proper plasma membrane localization.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri173 – 22351DHHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ERF2 (EC:2.3.1.225)
Alternative name(s):
DHHC cysteine-rich domain-containing protein ERF2
Ras protein acyltransferase
Gene namesi
Name:ERF2
Ordered Locus Names:YLR246W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR246W.
SGDiS000004236. ERF2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575CytoplasmicSequence analysisAdd
BLAST
Transmembranei76 – 9621HelicalSequence analysisAdd
BLAST
Topological domaini97 – 1048LumenalSequence analysis
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Topological domaini126 – 21792CytoplasmicSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Topological domaini239 – 25012LumenalSequence analysisAdd
BLAST
Transmembranei251 – 27121HelicalSequence analysisAdd
BLAST
Topological domaini272 – 35988CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum palmitoyltransferase complex Source: SGD
  • integral component of endoplasmic reticulum membrane Source: SGD
  • perinuclear endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1731K → E in ERF2-1; loss of function. 1 Publication
Mutagenesisi182 – 1821R → Q in ERF2-7; loss of function. 1 Publication
Mutagenesisi189 – 1891C → S: Abolishes palmitoyltransferase activity and interaction with SHR5. 2 Publications
Mutagenesisi200 – 2001D → A: Loss of function. 1 Publication
Mutagenesisi201 – 2011H → A: Abolishes palmitoyltransferase activity. 1 Publication
Mutagenesisi203 – 2031C → S: Abolishes palmitoyltransferase activity. 1 Publication
Mutagenesisi218 – 2181F → S in ERF2-2; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Palmitoyltransferase ERF2PRO_0000212948Add
BLAST

Post-translational modificationi

Autopalmitoylated.

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Subunit structurei

Interacts with SHR5.2 Publications

Protein-protein interaction databases

BioGridi31513. 40 interactions.
DIPiDIP-4783N.
IntActiQ06551. 1 interaction.
MINTiMINT-568524.

Structurei

3D structure databases

ProteinModelPortaliQ06551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Contains 1 DHHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri173 – 22351DHHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074293.
HOGENOMiHOG000248576.
InParanoidiQ06551.
KOiK16675.
OMAiQQTTHEY.
OrthoDBiEOG7KWSTD.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. ZF_DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVSRRSTR SESTSITKEE HTGEGSLTKL FFRWLVTLEG DQDINDGKGY
60 70 80 90 100
ISLPNVSNYI FFLGGRFRTV KGAKPLWLGV LLAIVCPMVL FSIFEAHKLW
110 120 130 140 150
HTQNGYKVLV IFFYYFWVIT LASFIRTATS DPGVLPRNIH LSQLRNNYQI
160 170 180 190 200
PQEYYNLITL PTHSSISKDI TIKYCPSCRI WRPPRSSHCS TCNVCVMVHD
210 220 230 240 250
HHCIWVNNCI GKRNYRFFLI FLLGAILSSV ILLTNCAIHI ARESGGPRDC
260 270 280 290 300
PVAILLLCYA GLTLWYPAIL FTYHIFMAGN QQTTREFLKG IGSKKNPVFH
310 320 330 340 350
RVVKEENIYN KGSFLKNMGH LMLEPRGPSF VSARKPHEAG DWRFMDLSPA

HSFEKIQKI
Length:359
Mass (Da):41,099
Last modified:November 1, 1996 - v1
Checksum:iB1FF42A2370F064B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681R → M in AAT92932 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67393.1.
AY692913 Genomic DNA. Translation: AAT92932.1.
BK006945 Genomic DNA. Translation: DAA09560.1.
PIRiS59392.
RefSeqiNP_013347.1. NM_001182133.1.

Genome annotation databases

EnsemblFungiiYLR246W; YLR246W; YLR246W.
GeneIDi850947.
KEGGisce:YLR246W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67393.1.
AY692913 Genomic DNA. Translation: AAT92932.1.
BK006945 Genomic DNA. Translation: DAA09560.1.
PIRiS59392.
RefSeqiNP_013347.1. NM_001182133.1.

3D structure databases

ProteinModelPortaliQ06551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31513. 40 interactions.
DIPiDIP-4783N.
IntActiQ06551. 1 interaction.
MINTiMINT-568524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR246W; YLR246W; YLR246W.
GeneIDi850947.
KEGGisce:YLR246W.

Organism-specific databases

EuPathDBiFungiDB:YLR246W.
SGDiS000004236. ERF2.

Phylogenomic databases

GeneTreeiENSGT00550000074293.
HOGENOMiHOG000248576.
InParanoidiQ06551.
KOiK16675.
OMAiQQTTHEY.
OrthoDBiEOG7KWSTD.

Enzyme and pathway databases

BioCyciYEAST:G3O-32351-MONOMER.

Miscellaneous databases

PROiQ06551.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. ZF_DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Erf2, a novel gene product that affects the localization and palmitoylation of Ras2 in Saccharomyces cerevisiae."
    Bartels D.J., Mitchell D.A., Dong X., Deschenes R.J.
    Mol. Cell. Biol. 19:6775-6787(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-173; ARG-182; CYS-189; ASP-200 AND PHE-218, SUBCELLULAR LOCATION.
  5. "Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae."
    Lobo S., Greentree W.K., Linder M.E., Deschenes R.J.
    J. Biol. Chem. 277:41268-41273(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-189; HIS-201 AND CYS-203, INTERACTION WITH SHR5.
  6. "Erf4p and Erf2p form an endoplasmic reticulum-associated complex involved in the plasma membrane localization of yeast Ras proteins."
    Zhao L., Lobo S., Dong X., Ault A.D., Deschenes R.J.
    J. Biol. Chem. 277:49352-49359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHR5, SUBCELLULAR LOCATION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiERFB_YEAST
AccessioniPrimary (citable) accession number: Q06551
Secondary accession number(s): D6VYP4, Q6B217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.