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Protein

Palmitoyltransferase ERF2

Gene

ERF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The ERF2-SHR5 complex is a palmitoyltransferase specific for Ras proteins. Palmitoylates RAS2, which is required for its proper plasma membrane localization.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-32351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ERF2 (EC:2.3.1.225)
Alternative name(s):
DHHC cysteine-rich domain-containing protein ERF2
Ras protein acyltransferase
Gene namesi
Name:ERF2
Ordered Locus Names:YLR246W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR246W.
SGDiS000004236. ERF2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 104LumenalSequence analysis8
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 217CytoplasmicSequence analysisAdd BLAST92
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Topological domaini239 – 250LumenalSequence analysisAdd BLAST12
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21
Topological domaini272 – 359CytoplasmicSequence analysisAdd BLAST88

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum palmitoyltransferase complex Source: SGD
  • integral component of endoplasmic reticulum membrane Source: SGD
  • perinuclear endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173K → E in ERF2-1; loss of function. 1 Publication1
Mutagenesisi182R → Q in ERF2-7; loss of function. 1 Publication1
Mutagenesisi189C → S: Abolishes palmitoyltransferase activity and interaction with SHR5. 2 Publications1
Mutagenesisi200D → A: Loss of function. 1 Publication1
Mutagenesisi201H → A: Abolishes palmitoyltransferase activity. 1 Publication1
Mutagenesisi203C → S: Abolishes palmitoyltransferase activity. 1 Publication1
Mutagenesisi218F → S in ERF2-2; loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129481 – 359Palmitoyltransferase ERF2Add BLAST359

Post-translational modificationi

Autopalmitoylated.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PRIDEiQ06551.

Interactioni

Subunit structurei

Interacts with SHR5.2 Publications

Protein-protein interaction databases

BioGridi31513. 41 interactors.
DIPiDIP-4783N.
IntActiQ06551. 1 interactor.
MINTiMINT-568524.

Structurei

3D structure databases

ProteinModelPortaliQ06551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 223DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Contains 1 DHHC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074293.
HOGENOMiHOG000248576.
InParanoidiQ06551.
KOiK16675.
OMAiFCTNVAR.
OrthoDBiEOG092C4JRP.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVSRRSTR SESTSITKEE HTGEGSLTKL FFRWLVTLEG DQDINDGKGY
60 70 80 90 100
ISLPNVSNYI FFLGGRFRTV KGAKPLWLGV LLAIVCPMVL FSIFEAHKLW
110 120 130 140 150
HTQNGYKVLV IFFYYFWVIT LASFIRTATS DPGVLPRNIH LSQLRNNYQI
160 170 180 190 200
PQEYYNLITL PTHSSISKDI TIKYCPSCRI WRPPRSSHCS TCNVCVMVHD
210 220 230 240 250
HHCIWVNNCI GKRNYRFFLI FLLGAILSSV ILLTNCAIHI ARESGGPRDC
260 270 280 290 300
PVAILLLCYA GLTLWYPAIL FTYHIFMAGN QQTTREFLKG IGSKKNPVFH
310 320 330 340 350
RVVKEENIYN KGSFLKNMGH LMLEPRGPSF VSARKPHEAG DWRFMDLSPA

HSFEKIQKI
Length:359
Mass (Da):41,099
Last modified:November 1, 1996 - v1
Checksum:iB1FF42A2370F064B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68R → M in AAT92932 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67393.1.
AY692913 Genomic DNA. Translation: AAT92932.1.
BK006945 Genomic DNA. Translation: DAA09560.1.
PIRiS59392.
RefSeqiNP_013347.1. NM_001182133.1.

Genome annotation databases

EnsemblFungiiYLR246W; YLR246W; YLR246W.
GeneIDi850947.
KEGGisce:YLR246W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20865 Genomic DNA. Translation: AAB67393.1.
AY692913 Genomic DNA. Translation: AAT92932.1.
BK006945 Genomic DNA. Translation: DAA09560.1.
PIRiS59392.
RefSeqiNP_013347.1. NM_001182133.1.

3D structure databases

ProteinModelPortaliQ06551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31513. 41 interactors.
DIPiDIP-4783N.
IntActiQ06551. 1 interactor.
MINTiMINT-568524.

Proteomic databases

PRIDEiQ06551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR246W; YLR246W; YLR246W.
GeneIDi850947.
KEGGisce:YLR246W.

Organism-specific databases

EuPathDBiFungiDB:YLR246W.
SGDiS000004236. ERF2.

Phylogenomic databases

GeneTreeiENSGT00550000074293.
HOGENOMiHOG000248576.
InParanoidiQ06551.
KOiK16675.
OMAiFCTNVAR.
OrthoDBiEOG092C4JRP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32351-MONOMER.

Miscellaneous databases

PROiQ06551.

Family and domain databases

InterProiIPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF01529. zf-DHHC. 1 hit.
[Graphical view]
PROSITEiPS50216. DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERFB_YEAST
AccessioniPrimary (citable) accession number: Q06551
Secondary accession number(s): D6VYP4, Q6B217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.