ID   CDD_YEAST               Reviewed;         142 AA.
AC   Q06549;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 88.
DE   RecName: Full=Cytidine deaminase;
DE            Short=CDA;
DE            EC=3.5.4.5;
DE   AltName: Full=Cytidine aminohydrolase;
GN   Name=CDD1; OrderedLocusNames=YLR245C; ORFNames=L9672.13;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX   MEDLINE=99431800; PubMed=10501935; DOI=10.1007/s002940050482;
RA   Kurtz J.-E., Exinger F., Erbs P., Jund R.;
RT   "New insights into the pyrimidine salvage pathway of Saccharomyces
RT   cerevisiae: requirement of six genes for cytidine metabolism.";
RL   Curr. Genet. 36:130-136(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313267; PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA   Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA   Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA   Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA   Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA   Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA   Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA   Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA   Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA   Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA   Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA   Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ZINC IONS, AND
RP   SUBUNIT.
RX   PubMed=15148397; DOI=10.1073/pnas.0400493101;
RA   Xie K., Sowden M.P., Dance G.S., Torelli A.T., Smith H.C.,
RA   Wedekind J.E.;
RT   "The structure of a yeast RNA-editing deaminase provides insight into
RT   the fold and function of activation-induced deaminase and APOBEC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:8114-8119(2004).
CC   -!- FUNCTION: This enzyme scavenge exogenous and endogenous cytidine
CC       and 2'-deoxycytidine for UMP synthesis.
CC   -!- CATALYTIC ACTIVITY: Cytidine + H(2)O = uridine + NH(3).
CC   -!- COFACTOR: Zinc.
CC   -!- SUBUNIT: Homodimer.
CC   -!- INTERACTION:
CC       Self; NbExp=2; IntAct=EBI-4455, EBI-4455;
CC       P32502:GCD7; NbExp=1; IntAct=EBI-4455, EBI-6260;
CC       Q02866:MUK1; NbExp=1; IntAct=EBI-4455, EBI-32646;
CC       Q02821:SRP1; NbExp=1; IntAct=EBI-4455, EBI-1797;
CC   -!- MISCELLANEOUS: Present with 3120 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family.
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DR   EMBL; AF080089; AAD04031.1; -; Genomic_DNA.
DR   EMBL; U20865; AAB67399.1; -; Genomic_DNA.
DR   EMBL; AY557930; AAS56256.1; -; Genomic_DNA.
DR   PIR; S59391; S59391.
DR   RefSeq; NP_013346.1; -.
DR   PDB; 1R5T; X-ray; 2.00 A; A/B/C/D=1-142.
DR   PDBsum; 1R5T; -.
DR   DIP; DIP:1252N; -.
DR   IntAct; Q06549; 6.
DR   PeptideAtlas; Q06549; -.
DR   PRIDE; Q06549; -.
DR   Ensembl; YLR245C; Saccharomyces cerevisiae.
DR   GeneID; 850946; -.
DR   GenomeReviews; Y13138_GR; YLR245C.
DR   KEGG; sce:YLR245C; -.
DR   NMPDR; fig|4932.3.peg.4359; -.
DR   CYGD; YLR245c; -.
DR   SGD; S000004235; CDD1.
DR   HOGENOM; Q06549; -.
DR   OMA; Q06549; YMTKLDG.
DR   BRENDA; 3.5.4.5; 250.
DR   NextBio; 967405; -.
DR   ArrayExpress; Q06549; -.
DR   GermOnline; YLR245C; Saccharomyces cerevisiae.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0004126; F:cytidine deaminase activity; IDA:SGD.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006216; P:cytidine catabolic process; IDA:SGD.
DR   GO; GO:0006217; P:deoxycytidine catabolic process; IDA:SGD.
DR   GO; GO:0008655; P:pyrimidine salvage; IDA:SGD.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_Zn_bd.
DR   InterPro; IPR006262; Cyt_deam_tetra.
DR   PANTHER; PTHR11644:SF2; Cyt_deam_tetra; 1.
DR   Pfam; PF00383; dCMP_cyt_deam_1; 1.
DR   TIGRFAMs; TIGR01354; cyt_deam_tetra; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Hydrolase; Metal-binding; Zinc.
FT   CHAIN         1    142       Cytidine deaminase.
FT                                /FTId=PRO_0000171685.
FT   REGION       50     52       Substrate binding (By similarity).
FT   ACT_SITE     63     63       Proton donor (By similarity).
FT   METAL        61     61       Zinc; catalytic.
FT   METAL        96     96       Zinc; catalytic.
FT   METAL        99     99       Zinc; catalytic.
FT   HELIX         8     21
FT   HELIX        22     24
FT   TURN         28     30
FT   STRAND       34     39
FT   STRAND       45     49
FT   HELIX        56     58
FT   HELIX        62     72
FT   STRAND       81     87
FT   STRAND       89     91
FT   HELIX        97    104
FT   STRAND      112    116
FT   STRAND      118    127
FT   HELIX       128    131
FT   HELIX       138    140
SQ   SEQUENCE   142 AA;  15536 MW;  962BD69CCB30D51F CRC64;
     MKVGGIEDRQ LEALKRAALK ACELSYSPYS HFRVGCSILT NNDVIFTGAN VENASYSNCI
     CAERSAMIQV LMAGHRSGWK CMVICGDSED QCVSPCGVCR QFINEFVVKD FPIVMLNSTG
     SRSKVMTMGE LLPMAFGPSH LN
//