Q06549 (CDD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytidine deaminase
Short name=CDA
EC=3.5.4.5

Alternative name(s):
Cytidine aminohydrolase

Gene names

Name:	CDD1
Ordered Locus Names:	YLR245C
ORF Names:	L9672.13

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Catalytic activity	Cytidine + H2O = uridine + NH3.
Cofactor	Zinc.
Subunit structure	Homodimer. Ref.6
Miscellaneous	Present with 3120 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	deoxycytidine catabolic process Inferred from direct assay Ref.1. Source: SGD pyrimidine-containing compound salvage Inferred from direct assay Ref.1. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	cytidine deaminase activity Inferred from direct assay Ref.1. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-4455,EBI-4455

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 142

142

Cytidine deaminase

PRO_0000171685

Regions

Region

50 – 52

3

Substrate binding By similarity

Sites

Active site

63

1

Proton donor By similarity

Metal binding

61

1

Zinc; catalytic

Metal binding

96

1

Zinc; catalytic

Metal binding

99

1

Zinc; catalytic

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

142

Helix Strand Turn

Details...

Helix

8 – 21

14

Helix

22 – 24

3

Turn

28 – 30

3

Beta strand

34 – 39

6

Beta strand

45 – 49

5

Helix

56 – 58

3

Helix

62 – 72

11

Beta strand

81 – 87

7

Beta strand

89 – 91

3

Helix

97 – 104

8

Beta strand

112 – 116

5

Beta strand

118 – 127

10

Helix

128 – 131

4

Helix

138 – 140

3

Sequences

	Sequence		Length	Mass (Da)
	Q06549 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 962BD69CCB30D51F	FASTA	142	15,536
	10 20 30 40 50 60 MKVGGIEDRQ LEALKRAALK ACELSYSPYS HFRVGCSILT NNDVIFTGAN VENASYSNCI 70 80 90 100 110 120 CAERSAMIQV LMAGHRSGWK CMVICGDSED QCVSPCGVCR QFINEFVVKD FPIVMLNSTG 130 140 SRSKVMTMGE LLPMAFGPSH LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism." Kurtz J.-E., Exinger F., Erbs P., Jund R. Curr. Genet. 36:130-136(1999) [PubMed: 10501935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1." Xie K., Sowden M.P., Dance G.S., Torelli A.T., Smith H.C., Wedekind J.E. Proc. Natl. Acad. Sci. U.S.A. 101:8114-8119(2004) [PubMed: 15148397] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF080089 Genomic DNA. Translation: AAD04031.1.
U20865 Genomic DNA. Translation: AAB67399.1.
AY557930 Genomic DNA. Translation: AAS56256.1.
BK006945 Genomic DNA. Translation: DAA09559.1.

PIR

S59391.

RefSeq

NP_013346.1. NM_001182132.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R5T	X-ray	2.00	A/B/C/D	1-142	[»]

ProteinModelPortal

Q06549.

SMR

Q06549. Positions 2-142.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1252N.

IntAct

Q06549. 11 interactions.

MINT

MINT-398236.

STRING

Q06549.

Proteomic databases

PeptideAtlas

Q06549.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR245C; YLR245C; YLR245C.

GeneID

850946.

KEGG

sce:YLR245C.

NMPDR

fig|4932.3.peg.4359.

Organism-specific databases

CYGD

YLR245c.

SGD

S000004235. CDD1.

Phylogenomic databases

eggNOG

fuNOG10856.

GeneTree

EFGT00050000006257.

HOGENOM

HBG352939.

OMA

KLVKMAL.

OrthoDB

EOG49CTJD.

Gene expression databases

ArrayExpress

Q06549.

Genevestigator

Q06549.

GermOnline

YLR245C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR006262. Cyt_deam_tetra.
IPR016193. Cytidine_deaminase-like.
[Graphical view]

KO

K01489.

Pfam

PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]

SUPFAM

SSF53927. Cytidine_deaminase-like. 1 hit.

TIGRFAMs

TIGR01354. Cyt_deam_tetra. 1 hit.

PROSITE

PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

967405.

Entry information

Entry name

CDD_YEAST

Accession

Primary (citable) accession number: Q06549
Secondary accession number(s): D6VYP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program