Reviewed, UniProtKB/Swiss-Prot Q06549 (CDD_YEAST)

Names and origin

Protein names

Recommended name:
    Cytidine deaminase
      Short name=CDA
    EC=3.5.4.5
Alternative name(s):
    Cytidine aminohydrolase

Gene names

Name:	CDD1
Ordered Locus Names:	YLR245C
ORF Names:	L9672.13

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Catalytic activity	Cytidine + H2O = uridine + NH3.
Cofactor	Zinc.
Subunit structure	Homodimer. Ref.5
Miscellaneous	Present with 3120 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cytidine catabolic process Ref.1 Inferred from direct assay. Source: SGD deoxycytidine catabolic process Ref.1 Inferred from direct assay. Source: SGD pyrimidine salvage Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	cytidine deaminase activity Ref.1 Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-4455,EBI-4455
GCD7	P32502	1	EBI-4455,EBI-6260
MUK1	Q02866	1	EBI-4455,EBI-32646
SRP1	Q02821	1	EBI-4455,EBI-1797

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 142

142

Cytidine deaminase

PRO_0000171685

Regions

Region

50 – 52

3

Substrate binding By similarity

Sites

Active site

63

1

Proton donor By similarity

Metal binding

61

1

Zinc; catalytic

Metal binding

96

1

Zinc; catalytic

Metal binding

99

1

Zinc; catalytic

Secondary structure

1

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142

Helix Strand Turn

Details...

Helix

8 – 21

14

Helix

22 – 24

3

Turn

28 – 30

3

Beta strand

34 – 39

6

Beta strand

45 – 49

5

Helix

56 – 58

3

Helix

62 – 72

11

Beta strand

81 – 87

7

Beta strand

89 – 91

3

Helix

97 – 104

8

Beta strand

112 – 116

5

Beta strand

118 – 127

10

Helix

128 – 131

4

Helix

138 – 140

3

Sequences

	Sequence		Length	Mass (Da)
	Q06549-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 962BD69CCB30D51F	FASTA	142	15,536
	10 20 30 40 50 60 MKVGGIEDRQ LEALKRAALK ACELSYSPYS HFRVGCSILT NNDVIFTGAN VENASYSNCI 70 80 90 100 110 120 CAERSAMIQV LMAGHRSGWK CMVICGDSED QCVSPCGVCR QFINEFVVKD FPIVMLNSTG 130 140 SRSKVMTMGE LLPMAFGPSH LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism." Kurtz J.-E., Exinger F., Erbs P., Jund R. Curr. Genet. 36:130-136(1999) [PubMed: 10501935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1." Xie K., Sowden M.P., Dance G.S., Torelli A.T., Smith H.C., Wedekind J.E. Proc. Natl. Acad. Sci. U.S.A. 101:8114-8119(2004) [PubMed: 15148397] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF080089 Genomic DNA. Translation: AAD04031.1.
U20865 Genomic DNA. Translation: AAB67399.1.
AY557930 Genomic DNA. Translation: AAS56256.1.

PIR

S59391.

RefSeq

NP_013346.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R5T	X-ray	2.00	A/B/C/D	1-142	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1252N.

IntAct

Q06549. 11 interactions.

STRING

Q06549.

Proteomic databases

PeptideAtlas

Q06549.

PRIDE

Q06549.

Genome annotation databases

Ensembl

YLR245C; YLR245C; YLR245C; Saccharomyces cerevisiae. [Genome view]

GeneID

850946.

GenomeReviews

Gene locus YLR245C in contig Y13138_GR.

KEGG

sce:YLR245C.

NMPDR

fig|4932.3.peg.4359.

Organism-specific databases

CYGD

YLR245c.

SGD

S000004235. CDD1.

Phylogenomic databases

HOGENOM

Q06549.

OMA

YMTKLDG.

Enzyme and pathway databases

BRENDA

3.5.4.5. 250.

Gene expression databases

ArrayExpress

Q06549.

Genevestigator

Q06549.

GermOnline

YLR245C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn_bd.
IPR006262. Cyt_deam_tetra.
[Graphical view]

PANTHER

PTHR11644:SF2. Cyt_deam_tetra. 1 hit.

Pfam

PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01354. cyt_deam_tetra. 1 hit.

PROSITE

PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

967405.

Entry information

Entry name

CDD_YEAST

Accession

Primary (citable) accession number: Q06549

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)